ABSTRACT
The collections of varieties of spring barley cultivars from the Eastern European and Central Asian areas were analyzed by exonpecific PCR (EPIC) for beta-amylase genes. The endosperm beta-amylase gene (bamyl) was differentiated by the presence of 126 bp MITE insertion into intron 3 that is associated with low activity beta-amylase. The findings suggest that a low level of genetic variation for bamylgene within climatic zones is associated with individual breeding program for each climatic zone.
Subject(s)
Alleles , Endosperm/genetics , Gene Expression Regulation, Plant , Hordeum/genetics , Plant Proteins/genetics , beta-Amylase/genetics , Breeding , Endosperm/metabolism , Exons , Genetic Variation , Hordeum/metabolism , Humans , Introns , Mutagenesis, Insertional , Plant Proteins/metabolism , Siberia , beta-Amylase/metabolismABSTRACT
Allelic diversity in a set of 99 spring and winter barley varieties for the different direction of use (brewing, cereal and valuable) has been studied studied. PCR analyses with beta-amylase DNA-marker have shown that the genotypes of different barley varieties can include different alleles of beta-AMY1 gene.
