[Application of the automatic Edman procedure to establishing the primary structure of swine pepsinogen and its fragments]. / Primenenie avtomaticheskogo metoda Edmana dlia ustanovleniia pervichnoi struktury svinogo pepsinogena, pepsina i ego fragmentov

The automatic Edman procedure was applied to elucidate N-terminal sequences of swine pepsinogen, pepsin, and the fragments of its degradation by BrCN, i. e. B-1 and B-5. A "Beckman" model 890 instrument was used in experiments. 50 amino acid residues were split off the pepsinogen molecule and identified and 55 amino acid residues-off the pepsin molecule by means of gas chromatography. A continuous N-terminal sequence of pepsinogen was 119 amino acids, in which the overlapping of the known peptide sequences with enzymic hydrolysers was taken into account. In B-1, B-4, B-5 fragments 22, 31 and 38, residues, respectively were analyzed with the sequencer.