ABSTRACT
In the present study we evaluated in vitro immunoglobulin production from IgAD individuals and healthy controls. Peripheral blood mononuclear cells (PBMC) from IgAD and controls were cultured with anti-CD40 MoAb presented on a CDw32-transfected fibroblast cell line (CD40 system) in the presence of IL-10, IL-2, IL-4, transforming growth factor-beta (TGF-beta) alone as well as of IL-10 in combination with each of the other three cytokines. Only IL-10 added alone induced significant changes in baseline immunoglobulin production; marked increases in median supernatant levels of all three isotypes were observed in both groups. The most striking finding of this study was the synergizing effect of IL-4 on IgA production in the IgAD group when added with IL-10; median IgA supernatant level increased to a value superimposable on that found in the normal controls which remained about the same as when stimulated with IL-10 alone. The synergic effect of IL-4 and IL-10 was specific to the IgA isotype.
Subject(s)
IgA Deficiency/immunology , Interleukin-10/pharmacology , Interleukin-4/pharmacology , Adolescent , Adult , CD40 Antigens/immunology , Child , Child, Preschool , Drug Synergism , Female , Fibroblasts/immunology , Humans , IgA Deficiency/drug therapy , Interleukin-10/immunology , Interleukin-10/therapeutic use , Interleukin-2/immunology , Interleukin-2/pharmacology , Interleukin-4/immunology , Interleukin-4/therapeutic use , Male , Middle Aged , Transfection , Transforming Growth Factor beta/immunology , Transforming Growth Factor beta/pharmacologySubject(s)
Maternal-Fetal Exchange/immunology , Severe Combined Immunodeficiency/immunology , Th2 Cells/immunology , Apoptosis , Base Sequence , Cytokines/genetics , DNA Primers/genetics , Female , Gene Expression , Humans , Infant , Phenotype , Pregnancy , RNA, Messenger/genetics , RNA, Messenger/metabolism , Receptors, Antigen, T-Cell, alpha-beta/genetics , Severe Combined Immunodeficiency/genetics , Severe Combined Immunodeficiency/pathology , Th2 Cells/metabolism , Th2 Cells/pathologyABSTRACT
PURPOSE: To ascertain the best surgical technique for fixating an intraocular lens (IOL) in the posterior chamber in the absence of capsular support. SETTING: From the Institute of Ophthalmology, Pavia University, Pavia, Italy. METHODS: Using the SFVT System, the phases of IOL fixation were evaluated in cadaver eyes. In addition, IOLs were implanted in the posterior chamber in 340 eyes of patients without capsular support. One of several IOL fixation methods was used; IOL power was calculated using the same parameters as when the lens is positioned in the sulcus. Mean follow-up was 3.5 years (range 2 months to 5 years). RESULTS: Intraocular lens placement was accurate only when the loops were positioned in the sulcus, with the best approach being internal with suspension sutures placed at 3 and 9 o'clock. In most cases, refractive results were good; 152 eyes were within +/- 1.00 diopter of the predicted value. CONCLUSION: In eyes without capsular support, IOL implantation in the sulcus was possible only when sutures were placed exactly through the sulcus. An internal approach using suspension sutures at 3 and 9 o'clock gave the best results.
Subject(s)
Lens Capsule, Crystalline/surgery , Lenses, Intraocular , Sclera/surgery , Suture Techniques , Cataract Extraction/methods , Ciliary Body/anatomy & histology , Ciliary Body/surgery , Follow-Up Studies , Humans , Iris/anatomy & histology , Iris/surgery , Postoperative Complications , Refraction, Ocular , Sclera/anatomy & histology , Treatment OutcomeABSTRACT
We investigated the various segments of optic nerve microangioarchitecture in the aging eye. Some clinical results were obtained.
Subject(s)
Aging/physiology , Optic Nerve/blood supply , Aged , Aged, 80 and over , Female , Humans , Ischemia/etiology , Male , MicrocirculationABSTRACT
A primary haemangiopericytoma of the lacrimal sac in a 45-year-old male is reported. The neoplasm, highly unusual in this location, recurred twice before being correctly identified. Total excision with surrounding tissue appears to be the treatment of choice for this tumour, the behaviour of which is often unpredictable.
