ABSTRACT
The increasing interest in miniaturization of biological and chemical experiments or assays demands precise metering of the smallest amounts of reagents, e.g., on a planar substrate. Very sophisticated spotting systems can nowadays produce arrays of many thousands of different substances on an area of a few square inches. Such micro arrays and the technology behind them have become an important tool in genomic expression assays, proteomic applications, and even in the field of combinatoric chemistry. We present a technique to dispense the smallest amounts of fluids in the form of either simple spots or more complicated microarrays, where we use surface acoustic waves in combination with a predetermined surface chemistry. In addition to a detailed description of the technique, several examples of applications are presented.
Subject(s)
Acoustics/instrumentation , Microchemistry/instrumentation , Microfluidic Analytical Techniques/instrumentation , Nanotechnology/instrumentation , Specimen Handling/instrumentation , Equipment Design , Equipment Failure Analysis , Microchemistry/methods , Microfluidic Analytical Techniques/methods , Microfluidics/instrumentation , Microfluidics/methods , Specimen Handling/methodsABSTRACT
Surface acoustic waves are used to actuate and process smallest amounts of fluids on the planar surface of a piezoelectric chip. Chemical modification of the chip surface is employed to create virtual wells and tubes to confine the liquids. Lithographically modulated wetting properties of the surface define a fluidic network, in analogy to the wiring of an electronic circuit. Acoustic radiation pressure exerted by the surface wave leads to internal streaming in the fluid and eventually to actuation of small droplets along predetermined trajectories. This way, in analogy to microelectronic circuitry, programmable biochips for a variety of assays on a chip have been realized.
