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J Clin Microbiol ; 39(9): 3135-9, 2001 Sep.
Article in English | MEDLINE | ID: mdl-11526141

ABSTRACT

By using a rapid test for respiratory syncytial virus (RSV) detection (Abbott TestPack RSV), a number of patients were observed, showing repeatedly positive results over a period of up to 10 weeks. A prospective study was initiated to compare the rapid test with an antigen capture enzyme immunoassay (EIA) and a nested reverse transcriptase PCR (RT-PCR) protocol for detection of RSV serotypes A and B. Only respiratory samples from children exhibiting the prolonged presence of RSV (> or =5 days) as determined by the rapid test were considered. A total of 134 specimens from 24 children was investigated by antigen capture EIA and nested RT-PCR. Using RT-PCR as the reference method, we determined the RSV rapid test to have a specificity of 63% and a sensitivity of 66% and the antigen capture EIA to have a specificity of 96% and a sensitivity of 69% for acute-phase samples and the homologous virus serotype A. In 7 (29%) of 24 patients, the positive results of the RSV rapid test could not be confirmed by either nested RT-PCR or antigen capture EIA. In these seven patients a variety of other respiratory viruses were detected. For general screening the RSV rapid test was found to be a reasonable tool to get quick results. However, its lack of specificity in some patients requires confirmation by additional tests to rule out false-positive results and/or detection of other respiratory viruses.


Subject(s)
Respiratory Syncytial Virus Infections/diagnosis , Respiratory Syncytial Viruses/classification , Respiratory Syncytial Viruses/isolation & purification , Reverse Transcriptase Polymerase Chain Reaction , Adolescent , Child , Child, Preschool , Hospitals , Humans , Immunoenzyme Techniques/methods , Infection Control/methods , Nasopharynx/virology , Prospective Studies , Reagent Kits, Diagnostic , Respiratory Syncytial Virus Infections/virology , Sensitivity and Specificity
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