ABSTRACT
The ongoing effort to eradicate pathogenic bacteria and viruses is a major endeavor that requires development of new and innovative materials. Materials based on photodynamic action represent an emerging and attractive area of research, and therefore, a broad understanding of chemical design principles is required. In the present study, we investigated the antibacterial and antiviral activities of five different nanofibrous membranes composed of poly(vinyl alcohol) or poly(vinyl alcohol)-chitosan mixture cross-linked through silicon(IV)phthalocyanine derivative with the aim to identify the role of the carrier polymer and photosensitizers concentration on its efficacy. A straightforward cross-linking process was adopted to create a water-stable material with an almost uniform distribution of the fiber structure, as revealed by scanning electron microscopy. The results of the antimicrobial studies showed that the increase in the amount of chitosan in the polymer mixture, rather than the increase in the photosensitizer concentration, enhanced the activity of the material. Due to their visible light-triggered antimicrobial activity, the resulting materials provide valuable opportunities for both topical antimicrobial photodynamic therapy and the area of environmental remediation.
Subject(s)
Anti-Infective Agents , Chitosan , Nanofibers , Nanostructures , Anti-Bacterial Agents/chemistry , Anti-Bacterial Agents/pharmacology , Anti-Infective Agents/pharmacology , Chitosan/chemistry , Chitosan/pharmacology , Indoles/pharmacology , Isoindoles , Nanofibers/chemistry , Photosensitizing Agents/pharmacology , Polymers/pharmacology , Polyvinyl Alcohol/chemistryABSTRACT
Interest in the photodynamic inactivation of bacteria as an alternative method to antibiotic treatment continues to grow. Based on this approach, light-activated anti-infective interfaces could be fabricated via incorporation of photosensitizers into the polymer-based materials. In order to combine photobactericidal and antifouling functions, the choice of the carrier polymer is of particular significance: it should enable fast and effective conjugation of photosensitizer and reduce the formation of bioburden on the artificial material in a biological environment. This study reports one-pot fabrication and characterization of two silicon(IV)phthalocyanine /poly(vinyl alcohol)-based electrospun mats. The method relies on the thermal cross-linking of components by esterification using sebacic acid as a cross-linking agent. Fabricated flexible mats showed photosensitizer-dependent antibacterial photoactivity against different Gram-positive bacteria with low cytotoxic effects on human fibroblasts and were effective against bacterial attachment, as an early step toward future biofilm formation. This work provides practical guidelines in developing photoactive materials and interfaces that can be used in nonadhesive wound dressings, food packaging, water, and air filtration.