ABSTRACT
Boiled and unboiled standardized detached bone fragments were compared in a canine model. The fragments were either left detached or fixed with a lag-screw. Additionally, various replacements were combined with transverse osteotomy followed by reduction and fixation with a six-hole neutralization plate based on the AO-principle. Follow-up was at 0-6 weeks, 6-12 weeks and 12-24 weeks. Examination of the specimens comprised radiology, histology, microradiography, fluorescence-microscopy and microangiography. Qualitative assessment indicated that boiled bone showed some delay in resorption and new bone formation when compared with unboiled bone after six weeks, but this difference was no longer discernible after 12 weeks. Semi-quantitative assessment revealed hardly any difference after six weeks. During the first few weeks, however, boiled fragments showed a clear delay of 10-14 days in resorption and new bone formation when compared with unboiled fragments. The clinical relevance is that detached bone fragments contaminated with street-refuse can be sterilized by boiling without harmful effects. Replacement of the boiled bone fragments shows a somewhat delayed, but otherwise normal resorption and new bone formation.
Subject(s)
Bone Regeneration , Bone Resorption , Bone and Bones/surgery , Fractures, Open/surgery , Sterilization , Animals , Bone and Bones/blood supply , Dogs , Femoral Fractures , Follow-Up Studies , Fracture Fixation, Internal , OsteotomyABSTRACT
Bacterially contaminated and uncontaminated standardized autogenous bone fragments were compared in an experimental canine model. Bacterial contamination was effected by immersing in a Staphylococcus aureus suspension of 6 X 10(8)/ml for 15 minutes. The fragments were replaced and either left detached or fixed with a lag screw. In a number of cases bone replacement was combined with transverse osteotomy followed by reduction and fixation with a six-hole neutralization plate based on the AO-principle. Follow-up carried out after six and 12 weeks, comprised radiological, histological, microradiographic, fluorescence microscopic and micro-angiographic investigation. Non-quantitative assessment indicated that the rate of resorption and new bone formation in the bacterially contaminated fragments definitely exceeded that of the uncontaminated fragments. Semi-quantitative findings seemed to confirm these conclusions. After 12 weeks this difference had virtually disappeared, except in loosely replaced infected fragments, which still showed more resorption and new bone formation. Integration of all fragments was good, there was no encapsulation with sequestration.
Subject(s)
Bone Resorption , Bone and Bones/surgery , Fractures, Open/surgery , Animals , Bone Regeneration , Bone and Bones/blood supply , Bone and Bones/microbiology , Dogs , Microradiography , Microscopy, Fluorescence , Staphylococcus aureusSubject(s)
Colonic Diseases/surgery , Intestinal Obstruction/surgery , Intestinal Pseudo-Obstruction/surgery , Adolescent , Adult , Aged , Colonoscopy , Female , Humans , Male , Methods , Middle AgedSubject(s)
Breast Neoplasms/diagnostic imaging , Mammography , Adult , Diagnostic Errors , False Positive Reactions , Female , Humans , Middle AgedSubject(s)
Humeral Fractures/therapy , Splints , Adolescent , Adult , Aged , Female , Follow-Up Studies , Humans , Humeral Fractures/diagnostic imaging , Male , Middle Aged , Polypropylenes , RadiographyABSTRACT
Between 1968 and 1973 55 patients with a pilonidal sinus were treated by the gluteal fascial method. Thirty-two patients could be investigated in a follow-up study. Eight recurrences were observed. The author concludes that in his department (in a large training clinic) this method proved unsatisfactory.
