Healing of interpositional autologous bone grafts after total maxillary osteotomy.

Healing of autologous interpositional bone grafts after total osteotomy in the Macaca fascicularis monkey was studied using clinical, histologic, microangiographic, and autoradiographic methods at varying intervals up to six months. Results indicated that the autologous graft was very osteogenic and incorporated by host bone. Graft revascularization became apparent at two weeks postoperatively, and bone healing time was between two to four weeks. A hypervascular response was present to at least the six-month healing stage. The palatal mucosa plus the facial gingiva provided an adequate nutrient pedicle for total maxillary osteotomies with interpositional bone grafting.

Conversion of monomeric human growth hormone and big growth hormone into different molecular weight forms in vitro and after injection into humans.

Monomeric human growth hormore (hGH) and big hGH obtained by a new purification procedure from human pituitary glands were examined for a possible conversion into different molecular weight forms under in vitro and in vivo conditions. Sephadex G-150 gel filtration was used for separation. Big or monomeric hGH was incubated in hormone-free serum or injected into normal individuals in order to induce a peak of immunoreactive hGH. Specimens from early and late stages of the surge were subjected to gel filtration. It could be shown that not only the big hGH is converted to the smaller form but that monomeric hGH is also recovered in two higher molecular weight regions by gel filtration in vitro and in vivo experiments. A significantly higher conversion of monomeric to big hGH was found in vitro. This suggests an inhibition of big hGH formation from monomeric hGH in normal individuals. There was no apparent time relation of big hGH formation from monomeric hGH in vivo. Regarding the conversion of big hGH, more of this material remained in its original form in vitro. The immunoreactivity recovered in the region of the very large form was found to be higher in vivo. The formation of the monomer from big hGH was similar under in vitro and in vivo conditions.