[Morpho-functional changes of splenic autotransplants at the different time intervals after the operation]. / Morfofunktsional'nye izmeneniia autotransplantov selezenki v raznye sroki poske operatsii.

This investigation was aimed at the study of splenic autotransplant cytoarchitectonics and cellular structures functional activity 3-60 days after the operation. The studies were carried out in 70 albino male rats including intact animals (n-10) and rats subjected to splenectomy and subsequent spleen heterotopic autotransplantation (n-60). Cryostat sections of transplants were processed for Falc and Cross luminescent-histochemical methods and were stained with hematoxylin and eosin. Biogenic amines content was measured using LUMAM-4 microscope with FMEL-1A spectrofluorimetric device. The number of luminescent cells in periarterial T-dependent zone was found to increase significantly after 7 days, while the unchanged lymphoid nodules were found in the zone of unaltered tissues. At day 15 "intrafollicular" luminescent granulocytes appeared in the newly formed lymphoid nodules. At days 15 to 45 the increased content of biogenic amines was detected in the "intrafollicular" cells, while the significant rise in the amount of amines was seen in the lymphocytes of nodule T-independent zone. It is suggested that the presence of intact nodules in transplant peripheral zone together with an increase in cell numbers in T-dependent zone, are the key events in the development of immune-correction effect of the transplanted spleen during the first two weeks after the operation.

[Accidental involution of the thymus after splenectomy (an experimental study)]. / Aktsidental'naia involiutsiia timusa posle splenéktomii (immunogistologicheskoe issledovanie).

Using immunological, general clinical and luminescent histochemical methods experimental study of immunological status and bioamine-containing thymus structures was performed on third, seventh and fifteenth postsplenectomy days in 90 outbred albino male rats. Splenectomy leads to significant changes in blood immunological parameters and morphofunctional state of thymus bioamine-containing structures. Significant increase of biogenic amines content in all thymus structures, leukocyte number, complement activity neutrophilic granulocytes phagocytic activity, were registered in 3 days. Further growth of bioamine content, decrease of cortical and medullary substances diameter, significant number growth of juvenile forms of mast cells were observed in 7 days. Essential decline in erythrocyte number, hemoglobin and IgG and IgA content, leukocytosis with leukemoid reaction development were defined in blood formula. On 15th postsplenectomy day maximal biogenic amine concentration, especially histamine was reached and further reduction in cortex and medulla as well as decline of all the immunoglobulins and decrease of neutrophilic granulocytes phagocytosis were determined. Thus, splenectomy and subsequent development of accidental involution leads to secondary immunodeficiency and severe anemia.

[Changes in the neuromediator system of the thymus in rats after splenectomy]. / Izmeneniia neiromediatornoi sistemy timusa u krys posle splenectomy.

Removal of spleen leads to compensatory activation of the function of central immunogenetic organ and increase of hyperplastic processes in it on d 3 postoperatively which is accompanied by growth of biogenic amine concentration, appearance of bioaminergic areas and lobules-satellites as well as formation of new cloe correlative links. Transitory inhibition of immunogenesis occurs on d 15 and d 30 peaking on d 45 following splenectomy. At this period glycosaminoglycan composition of mast cells and their functional activity changes which is manifested by appearance of juvenile T-0 forms and new metachromatic types of mast cells. In 2 months decrease of APUD cells number and their mono- and diamine content and weakening of correlative interconnections is accompanied by true significant disturbance in T lymphocytes differentiation, their release in peripheral blood and further insufficiency of cellular immunity.