ABSTRACT
Colorectal tumors were studied 5 months after carcinogen injection to outbred albino rats with secondary immunodeficiency by common histological and immunobiochemical methods with the use of monoclonal and polyclonal antibodies to Ki-67, Bcl-2, p53, and VEGF. Injection of carcinogen to intact animals led to the formation of adenocarcinomas with high and moderate cell differentiation in the colon, while injection of carcinogen to animals with immunodeficiency was associated with a 1.5-fold higher incidence of GRADE 2 and GRADE 3 adenocarcinomas with more aggressive morphological phenotype and appearance of distant metastases.
Subject(s)
Antibodies/therapeutic use , Colorectal Neoplasms/drug therapy , Colorectal Neoplasms/immunology , 1,2-Dimethylhydrazine/toxicity , Adenocarcinoma/drug therapy , Adenocarcinoma/immunology , Adenocarcinoma/metabolism , Animals , Biomarkers, Tumor/metabolism , Cell Differentiation/drug effects , Colorectal Neoplasms/metabolism , Intestine, Large/drug effects , Intestine, Large/metabolism , Ki-67 Antigen/metabolism , Proto-Oncogene Proteins c-bcl-2/antagonists & inhibitors , Proto-Oncogene Proteins c-bcl-2/metabolism , Rats , Tumor Suppressor Protein p53/antagonists & inhibitors , Tumor Suppressor Protein p53/metabolism , Vascular Endothelial Growth Factor A/antagonists & inhibitors , Vascular Endothelial Growth Factor A/metabolismABSTRACT
The thymus of outbred male rats 5 months after splenectomy (experimental secondary immunodeficiency) was studied by common histological and immunohistochemical methods using monoclonal and polyclonal antibodies to CD3, CD30, CD68, synaptophysin, to S100, p53, bcl-2, and Ki-67 proteins. Removal of the spleen led to acute involution of the thymic parenchyma, which was replaced by the adipose tissue and was associated with restructuring of the thymopoietic and nonthymopoietic components of the gland, changes in cellular composition and antigenic phenotype of the lobular cortical and medullary matter, and by reduction of cell proliferation.
Subject(s)
Immunologic Deficiency Syndromes/metabolism , Immunologic Deficiency Syndromes/pathology , Thymus Gland/metabolism , Thymus Gland/pathology , Animals , Animals, Outbred Strains , Cell Count , Cell Proliferation , Immunohistochemistry , Immunologic Deficiency Syndromes/etiology , Macrophages/pathology , Male , Phenotype , Rats , Splenectomy , Thymocytes/pathology , Thymocytes/physiologyABSTRACT
The aim of the study was to examine the histological and immunohistochemical characteristics of the thymus 5 months after the intraperitoneal administration of the carcinogen (1,2-dimethylhydrazine in the dose of 20 mg/kg once a week for 2 weeks). The study was conducted on 50 outbred albino male rats. Paraffin sections of the thymus were stained with hematoxylin-eosin and were processed by an immunohistochemical method using antibodies against CD3, CD30, CD68, synaptophysin, S-100 protein, p53, bcl-2, Ki-67, as well as against IgM and IgG. It was found that carcinogen administration resulted in the increased number of thymic cells expressing bcl-2, S-100 and Ki-67, active T-lymphocytes and thymopoietic microenvironment cells, as well as in the change of the correlation between medullary and cortical CD3(+)-thymocytes with a predominance of the latter. Thus, the malignant tumor, developing in the colon, on the one hand, inhibits the supply of the precursors of thymopoiesis to the thymus, while on the other--enhances the proliferation and differentiation of thymocytes into mature forms.
