ABSTRACT
Optical tweezers are employed to study the action of the histone-like protein from Thermotoga maritima (TmHU) on DNA at a single molecule level. Binding and disruption of TmHU to and from DNA are found to take place in discrete steps of 4-5 nm length and a net binding enthalpy of about 16kBT. This is in reasonable agreement with a microscopic model that estimates the extension of the binding sites of the protein and evaluates the energetics mainly for bending of the DNA in the course of interaction.
Subject(s)
Bacterial Proteins/chemistry , DNA-Binding Proteins/chemistry , DNA/chemistry , Models, Molecular , Thermotoga maritima/metabolism , DNA, Single-Stranded/chemistry , Optics and Photonics , Protein Binding , Protein Conformation , Thermotoga maritima/geneticsABSTRACT
Optical tweezers are microscopic tools with extraordinary precision in the determination of the position (±2 nm) of a colloid (diameter: â¼2.0 µm) in 3D-space and in the measurement of small forces in the range between 0.1 and 100 pN (pN=10-12 N). Experiments are reported in which single double-stranded (ds)-DNA chains of different length [2,000 base pairs (bp), 3,000, 4,000, and 6,000 bp] are spanned between two colloidal particles by use of appropriate molecular linkers. For the forces applied (≤40 pN) a fully reversible and well reproducible force-extension dependence is found. The data can be well described by both the worm-like chain model or by an approach developed by R. G. Winkler. For the resulting persistence length, a pronounced dependence on the ionic concentration in the surrounding medium is found.
