ABSTRACT
The cell's cytoskeleton together with the cell membrane and numerous accessory proteins determines the mechanical properties of cell. Any factors influencing cell organization and structure can cause alterations in mechanical properties of cell (its ability for deformation and adhesion). The determination of the local elastic properties of cells in their culture conditions has opened the possibility for the measurement of the influence of different factors on the mechanical properties of the living cells. The effect of the chitosan on the stiffness of the non-malignant transitional epithelial cells of ureter (HCV 29) and the transitional cell cancer of urine bladder (T24) was determined using scanning force microscopy. The investigations were performed in the culture medium (RPMI 1640) containing 10% fetal calf serum in the presence of the microcrystalline chitosan of the three different deacetylation degrees. In parallel, the effect of chitosan on production of lactate and ATP level was determined. The results showed the strong correlation between the decrease of the energy production and the increase in Young's modulus values obtained for the cancer cells treated with chitosan.
Subject(s)
Chitin/pharmacology , Urinary Bladder/drug effects , Cell Membrane/drug effects , Cells, Cultured , Chitin/analogs & derivatives , Chitosan , Cytoskeleton/drug effects , Glycolysis/drug effects , Humans , Microscopy, Atomic Force , Tumor Cells, CulturedABSTRACT
Arterial catheterization is one of the most frequently performed inpatient diagnostic and therapeutic procedures in the development countries. Complications may occur after any catheterization from inadequate hemostasis, particularly in the setting of aggressive anticoagulation. This study suggests that microcrystalline chitosan (MCCh) sealant installation via an arterial sheath at the completion of catheterization may improve hemostasis. Results using MCCh in eight heparinized dogs documented significant reductions in manual compression time (P = 0.016) of the artery after withdrawal of both the sheath introducer and catheter. Comparative results were found in rats, wherein a created wound in the aorta could be sealed relatively quickly and easily. The biodegradability, optimalization, and a better pharmaceutical formulation of this potential hemostatic agent require further studies.
Subject(s)
Biocompatible Materials/therapeutic use , Chitin/analogs & derivatives , Hemostatic Techniques , Hemostatics/therapeutic use , Animals , Aorta, Abdominal/cytology , Biocompatible Materials/chemistry , Catheterization/adverse effects , Chitin/chemistry , Chitin/therapeutic use , Chitosan , Dogs , Hemostatics/chemistry , Male , Punctures/adverse effects , Rats , Rats, Wistar , Tunica Intima/cytology , Vascular PatencyABSTRACT
The chemical pulping of wood without subsequent drying affords low-cost hollow fibers with microporous multilamellar cell walls saturated with water. Simple impregnation of these never-dried pulp fibers with solutions of biologically active chemicals followed by drying yields collapsed cellulose ribbons containing entrapped impregnant. The release characteristics of exemplary composites containing an analgesic (acetylsalicylic acid), a growth stimulant (alpha-naphthylacetic acid), or a herbicide (2,4-dichlorophenoxyacetic acid) are reported both for in vitro and in vivo evaluations.
