ABSTRACT
The influence of humic acid on the water chemistry of environmentally relevant concentrations of Al at neutral pH was studied, together with its effect on the bioavailability and toxicity of Al in Lymnaea stagnalis. Humic acid significantly reduced the loss of Al from the water and increased the fraction of filterable Al, although this was a relatively small fraction of total Al. Filterable Al concentration in the presence or absence of humic acid was independent of initial Al concentration. Humic acid only partly reduced toxicity, as observed by a reduction in behavioural suppression, and had no effect on the level of Al accumulated in tissues. These results suggest that humic acid maintains Al in a colloidal form that is bioavailable to L. stagnalis. However, these colloidal Al-humic acid species were less toxic since behavioural toxicity was reduced. Humic acid may play an important role in limiting the toxicity of Al to freshwater organisms.
Subject(s)
Aluminum/toxicity , Fresh Water/analysis , Humic Substances , Lymnaea/drug effects , Water Pollutants, Chemical/toxicity , Aluminum/pharmacokinetics , Animals , Behavior, Animal/drug effects , Biological Availability , Hydrogen-Ion Concentration , Lymnaea/physiology , Tissue Distribution/drug effectsABSTRACT
Aluminium accumulation by the freshwater snail Lymnaea stagnalis is correlated with behavioural depression which is ameliorated by addition of orthosilicic acid. We hypothesised that Si is relocated to the digestive gland in response to Al, leading to the formation of non-toxic hydroxyaluminosilicates (HAS). Exposure to 500 microg l(-1) Al for 30 days was associated with an initial period of behavioural depression, followed by apparent tolerance and subsequent depression, suggesting saturation of the cellular detoxification pathway during prolonged exposure. Exogenous Si (7.77 mg l(-1)) completely ameliorated all behavioural effects of Al but did not prevent its accumulation. In the presence of added Al, significantly more of this Si was accumulated by the tissues, compared to controls and snails exposed to Si alone. In snails exposed to Al plus Si, Al and Si concentrations were significantly correlated, with a ratio around 3:1 Al:Si, consistent with the presence of the non-toxic HAS protoimogolite.
Subject(s)
Aluminum/toxicity , Behavior, Animal/drug effects , Environmental Exposure/adverse effects , Silicon/physiology , Snails/drug effects , Water Pollution, Chemical/adverse effects , Aluminum/analysis , Animals , Behavior, Animal/physiology , Digestion , Fresh Water , Silicon/analysis , Silicon/pharmacology , Snails/metabolism , Snails/physiology , Water Pollution, Chemical/analysisABSTRACT
Atmospheric acidification of catchment-lake ecosystems may provide natural conditions for the in-lake control of P cycling. This process is based on the elevated transport of aluminum from acidified soils and its subsequent precipitation in the water body and is described for strongly acidified forest lakes, acidified and circumneutral reservoirs, and a moderately acidified alpine lake. In water bodies with episodically or permanently acidified inflows a pH gradient develops between lake water and tributaries due to: (i) neutralization of acidic inflows after mixing with waters with undepleted carbonate buffering system, and/or (ii) the in-lake alkalinity generation dominated by biochemical removal of NO3- and SO4(2-). With the pH increasing towards neutrality, ionic Al species hydrolyze and form colloidal Al hydroxides (Al(part)) with large specific surfaces and strong ability to bind orthophosphate from the liquid phase. Moreover, Alpart settles and increases the P sorption capacity of the sediment. The presence of Al(part) on the bottom reduces orthophosphate release from sediments after its liberation from ferric oxyhydroxides during anoxia because Al(part) is not sensitive to redox changes. Consequently, the natural in-lake P inactivation may be expected in any water body with elevated Al input and a pH gradient between its inlet and outlet.