ABSTRACT
A mathematical model based on principles of multifactor analysis was developed to predict clinical outcome of endometrial hyperplasia (EH) in patients with metabolic syndrome (80). Seventy-seven factors--anthropometric, clinical, anamnestic, hormono-metabolic, immunohistochemical, etc.--were included. Evaluation of the most informative indices integrated with the discriminative model showed that anthropometric (waist and hip circumference, sagittal diameter, etc.) and clinico-anamnestic (age, age of secondary sexual characters appearance, body weight at birth, suckling pattern, etc.) ones are of similar significance. A profile of hormono-metabolic parameters (cholesterol-low density lipoprotein, leptin, testosterone, progesterone and fasting glucose levels) helped identify a wide range of EH-related disorders in patients with metabolic syndrome. Consistently with the literature data, level of PTEN expression pointed to the presence of this tumor's suppressor in most EH cases which was matched by absence of its expression in endometrial carcinoma. Our model provided high sensitivity (89%) and specificity (82%) in predicting risk of progression in patients with endometrial hyperplasia and metabolic syndrome.
Subject(s)
Endometrial Hyperplasia/complications , Endometrial Hyperplasia/pathology , Endometrial Neoplasms/diagnosis , Endometrial Neoplasms/etiology , Metabolic Syndrome/complications , Models, Theoretical , Biomarkers/blood , Biomarkers, Tumor/analysis , Birth Weight , Body Weight , Cell Transformation, Neoplastic , Disease Progression , Endometrial Hyperplasia/blood , Endometrial Neoplasms/blood , Endometrial Neoplasms/chemistry , Endometrial Neoplasms/pathology , Female , Humans , Immunohistochemistry , Metabolic Syndrome/blood , Middle Aged , PTEN Phosphohydrolase/analysis , Predictive Value of Tests , Risk Assessment , Risk Factors , Sensitivity and Specificity , Waist CircumferenceABSTRACT
Sex hormone profile was investigated in uterine systemic and local blood flow in endometrial hyperplasia and carcinoma. The results were compared with levels of aromatase and steroid sulfatase - estrogen metabolism enzymes. Local hyperestradiolemia and hyperestronemia were detected in cancer patients. Estradiol and estrone in endometrial hyperplasia were lower than in cancer which conforms to estrogen theory of hormonal carcinogenesis. There was no evidence for the role of hyperestrogenemia in endometrial hyperplasia development. Aromatase was a factor of intensified estrogen synthesis in altered and hyperplastic endometrium. There was an inverse correlation between intratumoral steroid sulfatase levels and those of estrone in uterine local blood flow.
Subject(s)
Aromatase/metabolism , Endometrial Hyperplasia/blood , Endometrial Neoplasms/blood , Gonadal Steroid Hormones/blood , Steryl-Sulfatase/metabolism , Androstenedione/blood , Endometrial Hyperplasia/enzymology , Endometrial Neoplasms/enzymology , Estradiol/blood , Estrone/blood , Female , Humans , Middle Aged , Progesterone/blood , Regional Blood Flow , Sex Hormone-Binding Globulin/metabolismABSTRACT
Prognostic significance of sex hormones, sex hormone-binding globulin, estrogen and progesterone receptors as well as the activity of enzymes involved in estrogen synthesis (aromatase, steroid sulfatase, estrogen hydroxylase, catechol-O-methyl transferase, glutathione-S-transferase) were investigated in 74 endometrial carcinoma patients. Stage and duration of uterine myoma history appeared to be significant factors of overall survival. Such factors as stage, depth of myometrial invasion, body mass, blood-serum estradiol level as well as aromatase and catechol-O-methyl transferase concentration in tumor tissue were significant for free-relapse free survival period.
Subject(s)
Aromatase/metabolism , Catechol O-Methyltransferase/metabolism , Endometrial Neoplasms/metabolism , Estradiol Congeners/metabolism , Estrogens/biosynthesis , Leiomyoma/metabolism , Receptors, Estrogen/metabolism , Receptors, Progesterone/metabolism , Sex Hormone-Binding Globulin/metabolism , Disease-Free Survival , Endometrial Neoplasms/mortality , Endometrial Neoplasms/therapy , Female , Humans , Leiomyoma/mortality , Leiomyoma/therapy , Middle Aged , Neoplasm Staging , Survival RateABSTRACT
Activities of estrogen metabolism enzymes (aromatase, 2- and 4-estrogen hydroxylases, catechol-O-methyltransferase, and glutathione transferase) were studied by modern biochemical methods in tumors of patients with endometrial cancer. Relationships between enzyme activities and body weight index, age of menarche, stage of the disease, tumor histotype, differentiation degree, and depth of invasion into the myometrium were detected. The detected relationships between enzyme activities and serum concentrations of estradiol and progesterone and level of estrogen receptors in tumor tissue attest to hormone dependence of aromatase, estrogen hydroxylases, and glutathione transferase.
Subject(s)
Endometrial Neoplasms/metabolism , Estrogens/metabolism , Aromatase/metabolism , Aryl Hydrocarbon Hydroxylases/metabolism , Carcinoma in Situ/enzymology , Carcinoma in Situ/metabolism , Carcinoma in Situ/pathology , Catechol O-Methyltransferase/metabolism , Cytochrome P-450 CYP1A1/metabolism , Cytochrome P-450 CYP1B1 , Endometrial Neoplasms/enzymology , Endometrial Neoplasms/pathology , Female , Glutathione Transferase/metabolism , Humans , Middle Aged , Steroid Hydroxylases/metabolismABSTRACT
Total antiradical activity (ARA) of the blood serum, saliva, and gastric juice, level of nonenzymatic antioxidants, and activities of antioxidant enzymes were measured in patients with moderate and severe gastric dysplasia and cancer. Informative laboratory tests are suggested for singling out the high-risk group. Evaluation of the serum ARA, tocopherol, retinol, and superoxide dismutase/glutathion peroxidase coefficient helps detect patients with gastric cancer at a high risk of postoperative complications.
