Copper-Ruthenium Composite as Perspective Material for Bioelectrodes: Laser-Assisted Synthesis, Biocompatibility Study, and an Impedance-Based Cellular Biosensor as Proof of Concept.

Copper is an inexpensive material that has found wide application in electronics due to its remarkable electric properties. However, the high toxicity of both copper and copper oxide imposes restrictions on the application of this metal as a material for bioelectronics. One way to increase the biocompatibility of pure copper while keeping its remarkable properties is to use copper-based composites. In the present study, we explored a new copper-ruthenium composite as a potential biocompatible material for bioelectrodes. Sample electrodes were obtained by subsequent laser deposition of copper and ruthenium on glass plates from a solution containing salts of these metals. The fabricated Cu-Ru electrodes exhibit high effective area and their impedance properties can be described by simple R-CPE equivalent circuits that make them perspective for sensing applications. Finally, we designed a simple impedance cell-based biosensor using this material that allows us to distinguish between dead and alive HeLa cells.

Laser-Assisted Surface Modification of Ni Microstructures with Au and Pt toward Cell Biocompatibility and High Enzyme-Free Glucose Sensing.

We investigated the influence of morphology of Ni microstructures modified with Au and Pt on their cell biocompatibility and electrocatalytic activity toward non-enzymatic glucose detection. Synthesis and modification were carried out using a simple and inexpensive approach based on the method of laser-induced deposition of metal microstructures from a solution on the surface of various dielectrics. Morphological analysis of the fabricated materials demonstrated that the surface of the Ni electrode has a hierarchical structure with large-scale 10 µm pores and small-scale 10 nm irregularities. In turn, the Ni-Pt surface has large-scale cavities, small-scale pores (1-1.5 µm), and a few tens of nanometer particles opposite to Ni-Au that reveals no obvious hierarchical structure. These observations were supported by impedance spectroscopy confirming the hierarchy of the surface topography of Ni and Ni-Pt structures. We tested the biocompatibility of the fabricated Ni-based electrodes with the HeLa cells. It was shown that the Ni-Au electrode has a much better cell adhesion than Ni-Pt with a more complex morphology. On the contrary, porous Ni and Ni-Pt electrodes with a more developed surface area than that of Ni-Au have better catalytic performance toward enzymeless glucose sensing, revealing greater sensitivity, selectivity, and stability. In this regard, modification of Ni with Pt led to the most prominent results providing rather good glucose detection limits (0.14 and 0.19 µA) and linear ranges (10-300 and 300-1500 µA) as well as the highest sensitivities of 18,570 and 2929 µA mM-1 cm-2. We also proposed some ideas to clarify the observed behavior and explain the influence of morphology of the fabricated electrodes on their electrocatalytic activity and biocompatibility.

Bioimpedance Spectroscopy: Basics and Applications.

In this review, we aim to introduce the reader to the technique of electrical impedance spectroscopy (EIS) with a focus on its biological, biomaterials, and medical applications. We explain the theoretical and experimental aspects of the EIS with the details essential for biological studies, i.e., interaction of metal electrodes with biological matter and liquids, strategies of measurement rate increasing, noise reduction in bio-EIS experiments, etc. We also give various examples of successful bio-EIS practical implementations in science and technology, from whole-body health monitoring and sensors for vision prosthetic care to single living cell examination platforms, virus disease research, biomolecules detection, and implementation of novel biomaterials. The present review can be used as a bio-EIS tutorial for students as well as a handbook for scientists and engineers because of the extensive references covering the contemporary research papers in the field.

In Situ Laser-Induced Fabrication of a Ruthenium-Based Microelectrode for Non-Enzymatic Dopamine Sensing.

In this paper, we propose a fast and simple approach for the fabrication of the electrocatalytically active ruthenium-containing microstructures using a laser-induced metal deposition technique. The results of scanning electron microscopy and electrical impedance spectroscopy (EIS) demonstrate that the fabricated ruthenium-based microelectrode had a highly developed surface composed of 10 µm pores and 10 nm zigzag cracks. The fabricated material exhibited excellent electrochemical properties toward non-enzymatic dopamine sensing, including high sensitivity (858.5 and 509.1 µA mM-1 cm-2), a low detection limit (0.13 and 0.15 µM), as well as good selectivity and stability.