ABSTRACT
Steinernema weiseri n. sp. is described from a roadside with apple trees near Ceské Budejovice, Czech Republic. The species is also widely distributed in Germany and Slovakia, from where it had previously been reported as Steinernema spec. F. The British Steinernema sp. D1 is considered conspecific with S. weiseri n. sp. Males of the new species are mainly characterised by light brown, slightly curved spicules with a long manubrium and the presence of a short tail mucron in the second generation. Third-stage infective juveniles are characterised by a 'medium size' body and tail length, short hyaline tail portion (mostly around 1/3 of tail length), the excretory pore situated in the mid-pharynx region, lip region slightly offset, angular and flattened, and the lateral field having nine equally developed lines separated by eight distinct ridges. S. weiseri n. sp. is most similar to S. feltiae, with which it did not hybridise. RFLP analysis of the ITS region of the rDNA repeat shows S. weiseri n. sp. to be distinct from 50 other Steinernema species and isolates. The new species was found in a wide range of habitats and is readily maintained on Galleria mellonella larvae.
Subject(s)
Insecta/parasitology , Rhabditida/anatomy & histology , Soil/parasitology , Animals , Czech Republic , DNA, Helminth/chemistry , DNA, Helminth/genetics , DNA, Ribosomal/chemistry , DNA, Ribosomal/genetics , DNA, Ribosomal Spacer/chemistry , DNA, Ribosomal Spacer/genetics , Female , Male , Microscopy, Electron, Scanning , Polymerase Chain Reaction , Polymorphism, Restriction Fragment Length , Rhabditida/classification , Rhabditida/genetics , Rhabditida/ultrastructureABSTRACT
Forty soil samples from forests and other biotopes in Germany and the Czech Republic were studied for the presence of entomopathogenic nematodes using the Galleria bait method at the same time as a sieving-decanting method for direct extraction of infective-stage juveniles. Five Steinernema species were recovered from the samples from Germany and four species from the samples from Czechia. All five species were recovered with both methods, but the baiting technique was generally less effective and mixtures of species were frequently undetected. The direct extraction method provided quantitative estimates of infective-stage juvenile density but no information on their infectivity or on morphological characters of adults, and nematode cultures could not be established.
Subject(s)
Life Cycle Stages , Rhabditida/growth & development , Rhabditida/isolation & purification , Soil/parasitology , Animals , Czech Republic , Germany , Parasitology/methods , TreesABSTRACT
Longidorus iranicus n.sp. is described from the north-western part of Iran, where it was found in the rhizosphere of grape and other cultivated plants. Its main features are a 5-7 mm long and rather slender body, a narrow, rounded lip region which is almost continuous with neck contour, an odontostyle length around 110 µm, a short, bluntly conoid tail, the shape of the amphids, the position of the gland nuclei in the oesophageal bulb, the cuticle layering at the tail end and the absence of males. L. iranicus resembles L. goodeyi, L. caespiticola, L. taniwha, L. poessneckensis and, most closely, L. crassus. ac]19820617.
