Assessing impacts of municipal wastewater treatment plant upgrades on bacterial hazard contributions to the receiving urban river using SourceTracker.

Upgrading municipal wastewater treatment plants (MWTPs) has been implemented in many megacities of China to reduce the discharges of nutrients and other pollutants and improve water quality of highly urbanized rivers. However, the contribution of MWTP discharge to bacterial hazards in the receiving rivers after upgrades has been largely unknown. In this study, high-throughput sequencing and shotgun metagenomics were applied to investigate the changes in the abundance, composition, potential risks, and contributions of bacteria and antibiotic resistance genes (ARGs) from effluent to receiving river after upgrading the third-largest MWTP in China with denitrification biofilters, ultrafiltration, ozonation, and disinfection processes. The annual loadings of total nitrogen and 27 types of pharmaceuticals were reduced by 42.4% ± 13.2% and 46.2% ± 15.4%, respectively. Bacterial biomass decreased from (3.58 ± 0.49) to (1.23 ± 0.27) × 107 16S rRNA gene copies/mL, and identified biomarkers in effluent and downstream shifted due to the adopted processes. Opportunistic pathogen bacteria downstream were also reduced. Although the relative abundance of total ARGs in MWTP effluent increased from 1.10 ± 0.02 to 2.19 ± 0.03 copies/16S rRNA gene after upgrades, that of total and high-risk ARGs downstream showed no significant difference. More importantly, the Bayesian-based SourceTracker method provided valuable insight by revealing that the contributions of MWTP discharge to downstream bacteria (from 44.2% ± 1.5%-31.4% ± 0.9%) and ARGs (from 61.2% ± 5.3%-47.6% ± 4.1%) were significantly reduced following the upgrades, indicating upgrading MWTP showed integrated benefits to the bacterial hazards in the receiving river. This study provides useful information for better control of bacterial hazard risks and operational strategy for the improvement of the urban aquatic ecosystem.

Biodiversity and Interannual Variation of Harmful Algal Bloom Species in the Coastal Sea of Qinhuangdao, China.

For the frequent occurrence of harmful algal blooms (HABs) in the Qinhuangdao coastal sea (QCS) of the Bohai Sea in summer, we tested the hypothesis that high-biodiversity HAB species exist in the area, and a series of censuses of HAB species were conducted in the QCS in the summers of 2014-2019. Through morphological identification, we found 100 algae species representing 42 genera in 3 phytoplankton phyla in this study, among which Bacillariophyta was the most dominant phylum. We also found that the population density of Dinoflagellata increased from 2016 to 2019. In total, 59 HAB species were annotated in this study, including 39 of Bacillariophyta, 18 of Dinoflagellata and 2 of Ochrophyta, of which 13 HAB species were reported in the Bohai Sea for the first time, and most HAB species were widely distributed in the QCS in summer. Notably, four dominant HAB species displayed unique temporal and spatial distribution characteristics, while their distribution ranges and population densities increased from 2014 to 2019. The distributions of five environmental factors were different in the QCS, while the temperature, salinity, and dissolved inorganic nitrogen might be the key environmental factors influencing the distribution of dominant HAB species in the summer. In conclusion, this study provides a detailed evaluation of phytoplankton diversity and interannual variation in the QCS. The existence of a high level of biodiversity of algal bloom species suggests the need for long-term monitoring in order to further study and prevent potential HABs.

The complete mitochondrial genome and phylogenetic analysis of hydrozoan jellyfish Eirene ceylonensis (Cnidaria, Hydrozoa, Eirenidae) in the coastal sea of Qinhuangdao, China.

Eirene ceylonensis, a hydrozoan jellyfish species with a complex polymorphic life cycle, is widely distributed in the Chinese coastal sea. In this study, we conducted sequencing and analysis of the first complete mitochondrial genome of E. ceylonensis, obtained from the coastal sea of Qinhuangdao, China. The linear mitochondrial genome is 14,997 bp in length with the overall AT content being 72.8%, encoding 13 protein-coding genes (PCGs), two transfer RNA (tRNA) genes (tRNA-Met and tRNA-Trp) and two ribosomal RNA (rRNA) genes (rrnS and rrnL). Phylogenetic analysis of 13 PCGs suggests that the E. ceylonensis is closely related to Laomedea flexuosa. The availability of the complete mitochondrial genome of E. ceylonensis will be useful for studying the evolutionary relationships of hydrozoan jellyfish species.

Impacts of wastewater treatment plant upgrades on the distribution and risks of pharmaceuticals in receiving rivers.

Upgrades of wastewater treatment plant (WWTP) and full-scale application of additional advanced oxidation processes have been proven to be effective in reducing the nutrient emissions to the environment; however, the impacts of WWTP upgrades on the receiving waters with regard to the occurrence and ecological risks of pharmaceuticals are still unclear. In this study, 27 pharmaceuticals with diverse physicochemical properties were monitored in four rivers in Beijing, each of which was heavily impacted by a large-scale WWTP. Three-year sampling campaigns were conducted, covering the periods before and after the WWTP upgrades. The results show that the newly added combined treatment processes (e.g., biological filter, ultrafiltration, ozonation, and NaClO disinfection) reduced the total pharmaceutical concentrations in the effluents by 45-74%. The composition profiles reveal that the upgrades of two studied WWTPs resulted in a significant reduction of pharmaceutical concentrations in the receiving rivers, while little impacts were observed for the other rivers. The risk assessment shows that the acute toxic pressures in the studied rivers were generally low and the WWTP upgrades were conducive to reduce the risks for most of pharmaceuticals. However, erythromycin and ofloxacin still posed high risk, indicating the potential adverse effect of pharmaceuticals on aquatic environment.

Occurrence, source estimation and risk assessment of pharmaceuticals in the Chaobai River characterized by adjacent land use.

This study investigated the occurrence of 27 pharmaceuticals with diverse physicochemical properties in a year-long monitoring campaign in the Chaobai River, China. The correlation between the distribution of pharmaceuticals in the river and the adjacent sources was elucidated. The results indicate that the agriculture area was the most polluted area with a median summed pharmaceutical concentration of 225.3 ng L-1, followed by the urban area and the mountain area with the corresponding values of 136.9 and 29.9 ng L-1, respectively. In terms of individual compounds, 22 out of 27 compounds were detected with concentrations ranging from <1 to 1972 ng L-1. Caffeine, carbamazepine, azithromycin, bezafibrate, metoprolol, sulfadiazine, sulfamethoxazole, clarithromycin, erythromycin, roxithromycin, and trimethoprim were pharmaceuticals with relatively high levels, with median concentrations ranging from 3.3 to 25.6 ng L-1 and detection frequencies ranging from 40% to 97%. Higher concentrations were mainly observed during cold seasons, with mean concentrations 1 to 52 times as high as those during warm seasons. Spatial analysis reveals that the pharmaceutical concentrations in different areas were impacted by different sources. A wastewater treatment plant was an important source in the urban area, while the agriculture area was impacted by various treated and untreated wastewater sources. The species sensitivity distribution model and risk quotient (RQ) method were combined in the ecological risk assessment. The results indicate that the multi-substance potentially affected fraction (msPAF) values of the sampling sites were below 0.04%, whereas nearly half of RQ values were higher than 1. Caffeine was proposed as a priority compound due to its high contribution rate (i.e., 79%) to the cumulative msPAF value, which implies that increased control and management of untreated wastewater sources along the Chaobai River is necessary.

Analysis of Antiepileptic Drugs in6Children's Hospitals in the Yangtze Valley during the Period of2002～2004 / 中国药房

OBJECTIVE:To evaluate the status quo and the tendency of antiepileptic drugs used in6Children's Hospitals in the Yangtze Valley.METHODS:Data on drug variety,sales volume,manufacturers of antiepileptic drugs used in6Chil?dren's Hospitals in the Yangtze Valley during2002～2004were analyzed statistically.RESULTS:Sodium valproate and topi?ramate took a bigger share in the market with the proportion of both to the antiepileptic drugs in sales volume at about40%.The old variety still took the lead in the clinical drug consumption while the momentum for the consumption of new one was quite fine.CONCLUSION:There are only a few varieties of antiepileptics now yet the sales volume of which is on the rise,therefore,of which there is still prospect of development.

Determination of Glycyrrhizic Acid in Sulfonamide-glycyrrhiza Mixture by RP-HPLC / 中国药房

OBJECTIVE:To establish a RP-HPLC method for the determination of glycyrrhizic acid in sulfonamideglycyrrhiza mixture METHODS:Lichrosorb RPC18 analytical column was used The mobile phase was composed of methanol-(CH)4NH4OH(65∶35) The pH of the mobile phase was adjusted to 6 5 with 10% phosphoric acid Detection wavelength was 254nm RESULTS:Linearity was good in the range of 0 5～50?g/ml(r=0 9 996);the average recovery was 99 75%,RSD=1 87% CONCLUSION:The method is simple and convenient

To choose the prescription of the optimum testosterone propionate frost and the production craft condition with orthogonal test / 重庆医科大学学报

Objective:To choose the prescription of the optimum testosterone propionate frost and the production craft conditions.Methods:Take the stability and the external appearance forms of the creams as the index sign to be checked,and carry out the experiment with orthogonal test.With the oil matches each other the ratio,the creams dosage,the way to add the main medicine a variable factor.Results:The superior formulation and craft are A 1B 2C 2 or A 1B 3C 3.Conclusion:The ready frost of testosterone propionate meets the provision of the Chinese Medical Dictionary 2000 on relevant ointments.