ABSTRACT
We report the design and fabrication of a multifunctional persistent luminescent nanoprobe for imaging guided dual-stimulus responsive and triple-synergistic therapy of drug resistant tumor. The integration of the dual-stimulus of an acidic microenvironment and laser irradiation with the triple-synergistic therapy of doxorubicin, photothermal and siRNA offers excellent therapeutic performance for drug resistant tumor cells with high specificity and little side effects.
Subject(s)
Antitubercular Agents/therapeutic use , Combined Modality Therapy/methods , Doxorubicin/therapeutic use , Luminescent Agents/therapeutic use , RNA, Small Interfering/therapeutic use , Antitubercular Agents/administration & dosage , Antitubercular Agents/adverse effects , Cell Line, Tumor , Doxorubicin/administration & dosage , Doxorubicin/adverse effects , Drug Carriers/chemistry , Drug Liberation , Drug Resistance, Neoplasm , Drug Therapy/methods , Folic Acid/chemistry , Genetic Therapy/methods , Humans , Indoles/chemistry , Luminescent Agents/administration & dosage , Luminescent Agents/adverse effects , Nanoparticles/chemistry , Optical Imaging/methods , Particle Size , Phototherapy/methods , Polyethyleneimine/chemistry , Polymers/chemistry , RNA, Small Interfering/administration & dosage , RNA, Small Interfering/adverse effects , Surface PropertiesABSTRACT
We show a theranostic nanoplatform for messenger RNA (mRNA) triggered enhanced fluorescence imaging guided therapy. Catalytic hairpin assembly (CHA) and gold nanorods (AuNRs) are employed to fabricate the theranostic nanoplatform. Two hairpin DNAs and Cy5 labeled duplex DNA are integrated into the CHA for mRNA triggered fluorescence signal amplification via hybridization and displacement with mRNA. The AuNRs act both as the fluorescence quencher and the photothermal therapy (PTT) agent. The nanoplatform not only enables sensitive and specific imaging of target mRNA in living cells and good differentiating of the survivin mRNA expression levels in different cell lines but also offers excellent photothermal conversion efficiency for PTT. The developed nanoplatform has great potential for sensitive and specific intracellular mRNA imaging guided PTT.
Subject(s)
Gold/chemistry , Metal Nanoparticles/chemistry , Optical Imaging , Photosensitizing Agents/chemistry , Phototherapy , RNA, Messenger/chemistry , Catalysis , HumansABSTRACT
OBJECTIVE: To investigate the usefulness of electromyogram (EMG) in the diagnosis of the patients with hemimasticatory spasm (HMS). METHODS: Four cases with HMS were reported. All the 4 patients were undertaken needle and surface electrode EMG examination. RESULTS: Needle electrode EMG of the 4 patients with HMS showed grouped potentials synchronously with the onset of the spasm, which indicated abnormal excitatory electrical activities of the trigeminal nerve resulting in involuntary masticatory muscle movements. CONCLUSION: It is very important to use EMG for the diagnosis of HMS.
