ABSTRACT
BACKGROUND: Pancreatic cancer (PC) is an aggressive malignancy with poor prognosis. Accumulating studies have showed that long non-coding RNA (lncRNA) is a crucial regulator in various tumorigenesis and progression including PC. This research aims to explore the roles and molecular mechanism of lncRNA cancer susceptibility candidate 9 (CASC9) in PC. METHODS: The expression levels of lncRNA CASC9 and miR-497-5p were evaluated in PC tissues and paired adjacent healthy tissues by quantitative real-time PCR. PC cell lines were transfected with lentivirus targeting lncRNA CASC9, and cells proliferation, migration and invasion tests were conducted. Dual luciferase reporter assays were also carried out to explore the relationship between lncRNA CASC9, miR-497-5p and Cyclin D1 (CCND1). RESULTS: LncRNA CASC9 was significantly up-regulated in PC tissues, while miR-497-5p expression was down-regulated. Down-regulation of lncRNA CASC9 in PC cells can significantly suppress the cell aggressiveness both in vitro and in vivo; moreover, knock-down of miR-497-5p could neutralize this impact. Additionally, the luciferase activity assay has assured that CCND1 was a downstream target of miR-497-5p. CONCLUSION: LncRNA CASC9 can promote the PC progression by modulating miR-497-5p/CCND1 axis, which is potential target for PC treatment.
Subject(s)
MicroRNAs , Pancreatic Neoplasms , RNA, Long Noncoding , Humans , MicroRNAs/genetics , MicroRNAs/metabolism , RNA, Long Noncoding/genetics , RNA, Long Noncoding/metabolism , Cell Line, Tumor , Cyclin D1/genetics , Cyclin D1/metabolism , Pancreatic Neoplasms/genetics , Cell Proliferation/genetics , Cell Movement/genetics , Gene Expression Regulation, Neoplastic/genetics , Pancreatic NeoplasmsABSTRACT
BACKGROUND: Acute right-sided colonic diverticulitis (RCD) is a common disease in Asian populations for which the optimal treatment remains controversial. The aim of this study was to investigate management and evaluate long-term outcomes of treatment in patients with acute RCD. METHODS: We retrospectively collected and analyzed clinical data for patients with acute RCD admitted to the Tongren Hospital, Shanghai Jiao Tong University School of Medicine from December 2015 to December 2020. The patients were divided into two groups, according to primary treatment strategy, which was either conservative treatment or surgical treatment. RESULTS: A total of 162 consecutive patients with acute RCD were enrolled in the study. There was no significant difference in age, sex, history of abdominal surgery, medical co-morbidities, fever, previous history of RCD, treatment success rate and incidence of complications between the conservative and surgery groups. However, the recurrence rate in conservative groups was significantly higher than in surgery groups (16.53% vs 2.44%, P = 0.020). And more frequent bowel movements and previous history of RCD increased the risk of recurrence of acute RCD. Moreover, there was no significant difference in either treatment success rate or the overall recurrence rate between the patients with uncomplicated diverticulitis and patients with complicated diverticulitis. CONCLUSIONS: Surgical treatment is also safe and effective for acute RCD. Surgical treatment should mainly be considered for patients with acute RCD with recurrence risk factors (more frequent bowel movements and previous history of RCD) or with complicated acute RCD.
Subject(s)
Diverticulitis, Colonic , Diverticulitis , China , Diverticulitis/complications , Diverticulitis, Colonic/complications , Diverticulitis, Colonic/surgery , Humans , Recurrence , Retrospective Studies , Risk Factors , Treatment OutcomeABSTRACT
Gastric cancer is the fifth most lethal carcinoma in the world. Genetic and epigenetic factors transform the normal cells into malignant cells and lead to tumor development. MicroRNA (miRNA), a small non-coding RNA which functions in RNA silencing and post-transcriptional regulation of gene expression, is closely associated with cancer initiation and propagation, including stomach cancer. In this study, for the first time, we report miR-539-3P, as a tumor suppressor, was down-regulated in gastric cancer both in vivo and in vitro. In addition, dysfunction of miR-539-3P regulates gastric cancer cell proliferation and invasion. Bioinformatics analysis revealed CTBP1 is the direct target of miR-539-3P and high expression of CTBP1 faciliates the progression of gastric carcinoma through promoting the epithelial to mesenchymal transition (EMT). Overall, these results indicate that epigenetic regulation of CTBP1 through miR-539-3P is critical to gastric cancer and provide a new insight into gastric cancer diagnosis, treatment and prognosis.