[Preparation of 23-hydroxybetulinic acid from the roots of Pulsatilla chinensis].

OBJECTIVE: To isolate and purify high purity 23-hydroxybetulinic acid from the roots of Pulsatilla chinensis. METHODS: By acid hydrolysis, silica gel column chromatographies and recrystalization methods, 23-hydroxybetulinic acid was obtained from the roots of P. chinensis. RESULTS: High purity 23-hydroxylbetuline acid was isolated from the roots of P. chinensis, the contents is over 98. 0%. CONCLUSION: The preparation process is feasible and convenient. High purity 23-hyroxybetulinic acid could be used in the quality control of the materials and medicament of 23-hydroxybetulinic acid.