Variance of soil bacterial community and metabolic profile in the rhizosphere vs. non-rhizosphere of native plant Rumex acetosa L. from a Sb/As co-contaminated area in China.

Heavy metals (HMs) contamination poses a serious threat to soil health. However, the rhizosphere effect of native pioneer plants on the soil ecosystem remains unclear. Herein, how the rhizosphere (Rumex acetosa L.) influenced the process of HMs threatening soil micro-ecology was investigated by coupling various fractions of HMs, soil microorganisms and soil metabolism. The rhizosphere effect alleviated the HMs' stress by absorbing and reducing HMs' direct bioavailability, and the accumulation of ammonium nitrogen increased in the rhizosphere soil. Meanwhile, severe HMs contamination covered the rhizosphere effect on the richness, diversity, structure and predicted function pathways of soil bacterial community, but the relative abundance of Gemmatimonadota decreased and Verrucomicrobiota increased. The content of total HMs and physicochemical properties played a more important role than rhizosphere effect in shaping soil bacterial community. Furthermore, As was observed to have a more significant impact compared to Sb. Moreover, plant roots improved the stability of bacterial co-occurrence network, and significantly changed the critical genera. The process influenced bacterial life activity and nutrient cycling in soil, and the conclusion was further supported by the significant difference in metabolic profiles. This study illustrated that in Sb/As co-contaminated area, rhizosphere effect significantly changed soil HMs content and fraction, soil properties, and microbial community and metabolic profiles.

Responses of microbial communities and metabolic profiles to the rhizosphere of Tamarix ramosissima in soils contaminated by multiple heavy metals.

Heavy metals (HMs) contamination around smelters poses serious stress to soil microbiome. However, the co-effect of multiple HMs and native vegetation rhizosphere on the soil ecosystem remains unclear. Herein, effects of high HMs level and the rhizosphere (Tamarix ramosissima) on soil bacterial community structure and metabolic profiles in sierozem were analyzed by coupling high-throughput sequencing and soil metabolomics. Plant roots alleviated the threat of HMs by absorbing and stabilizing them in soil. High HMs level decreased the richness and diversity of soil bacterial community and increased numbers of special bacteria. Plant roots changed the contribution of HMs species shaping the bacterial community. Cd and Zn were the main contributors to bacterial distribution in non-rhizosphere soil, however, Pb and Cu became the most important HMs in rhizosphere soil. HMs induced more dominant metal-tolerant bacteria in non-rhizosphere than rhizosphere soil. Meanwhile, critical metabolites varied by rhizosphere in co-occurrence networks. Moreover, the same HMs-tolerant bacteria were regulated by different metabolites, e.g. unclassified family AKYG1722 was promoted by Dodecanoic acid in non-rhizosphere soil, while promoted by Octadecane, 2-methyl- in rhizosphere soil. The study illustrated that high HMs level and rhizosphere affected soil properties and metabolites, by which soil microbial community structure was reshaped.

Combined effects of oxytetracycline and microplastic on wheat seedling growth and associated rhizosphere bacterial communities and soil metabolite profiles.

The widespread application of antibiotics and plastic films in agriculture leads to new characteristics of soil pollution with the coexistence of antibiotics and microplastics. However, their combined effects on wheat seedling growth and associated rhizosphere bacterial communities and soil metabolite profiles remain unclear. Here, in the potted experiment, wheat was treated with individual oxytetracycline (0, 5.0, 50.0, and 150.0 mg kg-1) and the combination of oxytetracycline and polyethylene microplastic (0.2%). Results showed that 150 mg kg-1 oxytetracycline combined with microplastic significantly reduced the biomass and height of the plant. Compared with CK, all the treatments exposed to the combination of oxytetracycline and polyethylene microplastic significantly promoted carotenoid content and peroxidase activity in wheat leaves. Soil dehydrogenase and urease activities were more sensitive to current pollutant exposure than sucrase activity. Oxytetracycline (150 mg kg-1) alone and in combination with polyethylene significantly decreased the abundances of certain genera belonging to plant growth-promoting rhizobacteria (PGPR) in soil, such as Arthrobacter, Gemmatimonas, Massilia, and Sphingomonas. Combined exposure of 150 mg kg-1 oxytetracycline and polyethylene microplastic significantly altered multiple metabolites including organic acids and sugars. Network analysis indicated that co-exposure of 150 mg kg-1 oxytetracycline and microplastic may affect the colonization and succession of PGPR by regulating soil metabolites, thereby indirectly inhibiting wheat seedling growth. The results help to elucidate the potential mechanisms of phytotoxicity of the combination of oxytetracycline and polyethylene microplastic.

Impact of polystyrene nanoplastics (PSNPs) on seed germination and seedling growth of wheat (Triticum aestivum L.).

Microplastics and nanoplastics are emerging pollutants of global concern. However, the understanding of their ecological effects on terrestrial plants is still limited. We conducted the systematic research to reveal the impact of polystyrene nanoplastics (PSNPs) (0.01-10 mg/L) on seed germination and seedling growth of wheat (Triticum aestivum L.). The results showed that PSNPs had no discernible effect on seed germination rate whereas significantly (p < 0.01) increased root elongation by 88.6 %-122.6 % when compared with the control. Similarly, remarkable increases in carbon, nitrogen contents, and plant biomass were also observed after exposure to PSNPs. Moreover, PSNPs could reduce the shoot to root biomass ratio (S:R ratio) of wheat seedlings. Furthermore, the imagings of a 3D laser confocal scanning microscopy (LCSM) and scanning electron microscopy (SEM) indicated that PSNPs were taken up and subsequently down-top transported to shoot. The absorption and accumulation of four micronutrients (Fe, Mn, Cu and Zn) in wheat were generally reduced in varying degrees. Notably, metabolomics analysis revealed that all PSNPs treatments altered the leaf metabolic profiles mainly by regulating energy metabolisms and amino acid metabolisms. These findings are expected to provide new insights into the effects of PSNPs on crop plants.