Considerations and application status of selection marker for generation of recombinant biologics producing mammalian cell lines / 药学学报

The major challenge in the development of recombinant biologics lies in generating and isolating rare high-producing stable single clone in a short period of time. The selection marker is an essential component of the plasmid vector, it plays an important part in the generation and screening of producing cell lines. Engineering the selection marker to enhance the stringency of selection for high producing cells is one of the most effective approaches to improve the cell line development process. Here, using Chinese hamaster overy (CHO) cells as an example, we introduce the application of selection marker for generation of recombinant biologics producing mammalian cell lines, methods of engineering the selection markers to enhance the selection stringency, and propose considerations on cell substrate stability and selection marker safety, in order to provide references for high-efficiency development of recombinant biologics.

Research Progress of Enzyme Replacement Therapy for Rare Diseases Products and Their Pharmaceutical Assessment / 中国药学杂志

OBJECTIVE: To summarize the latest research progress in the development of enzyme replacement therapy drugs for rare diseases at home and abroad and their pharmaceutical assessment. METHODS: Based on the representative literature at home and abroad, this paper propose key points for consideration (such as molecular design, host selection, production process and quality control, etc.), as well as comments and suggestions for researcher of such products from review and evaluation perspective. RESULTS: Due to the complexity and heterogeneity of the drug molecules of enzyme replacement therapy for rare diseases, the quality research is the main difficulty in pharmaceutical development and evaluation. CONCLUSION: In recent years, in order to alleviate the lack of rare disease drugs in China and meet the urgent clinical needs, Chinese government has issued a series of policies to encourage the research and development of rare disease drugs.

[Photo-catalytic degradation of gas phase benzene and toluene over multi-composite TiO2 catalyst].

Photo-catalytic degradation of gas-phase benzene and toluene were studied in the condition of no catalyst, pure anatase catalyst, pure rutile catalyst and multi-composite catalyst. The influences of initial concentration of reactants and the catalysts of different composite on photo-catalytic degradation rate of gas-phase benzene and toluene were investigated. The results show that the degradation efficiency of benzene and toluene is improved a lot on anatase catalyst, but improved a little on rutile catalyst in relation to the crystal-structure of anatase and rutile catalyst. In the condition of no catalyst and pure rutile catalyst, toluene is easier to be degradated than benzene, and on anatase catalyst benzene is easier to be done than toluene. The initial concentration of reactant has an effect on the photo-catalytic degradation process. The degradation rates of benzene and toluene are faster in low concentration than in high concentration. A certain amount of rutile doped in anatase catalyst could improve the photo-catalytic activity. The catalyst with 80% anatase and 20% rutile shows the best photo-catalytic activity to benzene, and the catalyst with 90% anatase and 10% rutile gives the best photo-catalytic activity to toluene.

[Characteristics of atmospheric ozone in the urban area of Ji'nan].

Based on the observational data of near surface O3 in the urban area of Ji'nan during the latest two years, the O3 concentrations and their temporal variation characteristics were analyzed. The results showed that O3 concentrations fluctuated periodically,and the photochemical pollution in Ji'nan was serious. Diurnal variations of ozone concentrations exhibited with the characteristic of a single peak, and showed the maximum in the afternoon and minimum in the early morning before sunrise. Ozone concentrations in spring and summer were higher than autumn and winter, while the varying scopes of ozone concentrations in summer and autumn were broader than spring and winter. Variations of ozone concentrations in weekend days were different from weekdays, which caused by the temporal regulation of human activities.