ABSTRACT
By means of the polymerase chain reaction (PCR) it was obtained a probe for the gen that codifies the subunit B of cholerae toxin (CTxB), which carried a Vibrio cholerae 01 reference strain. The checking of the amplified product was performed by using the hybridization techniques in colonies. This product hybridized with the gen that codifies for the subunit B of cholerae toxin isolated from Peru and Ecuador, representing the present epidemics in Latin America, but it did not so with the phylogenetically related strains.
Subject(s)
Cholera Toxin/genetics , In Situ Hybridization/methods , Vibrio cholerae/genetics , Bacteriological Techniques , Ecuador , Genes, Bacterial , Humans , India , Peru , Polymerase Chain Reaction , Vibrio cholerae/isolation & purificationABSTRACT
Se reporta la correspondente encontrada entre los signos, síntomas y los niveles de los inmunocomplejos circulantes, los que van disminuyendo a medida que la evolucón del pacientes se hace más favorable, se discuten los resultados y de su análisis se sugiere la continuación de nuevas investigaciones en este campo.