Search | VHL Regional Portal

CyclosporineA augments endoplasmic reticulum stress markers and expression of matrix protein mRNA.

Rao, S R; Subbarayan, R; Girija, D M.

J Biol Regul Homeost Agents ; 31(1): 77-80, 2017.

Article in English | MEDLINE | ID: mdl-28337873

ABSTRACT

Cyclosporine-A induces gingival overgrowth with disturbance in the homeostasis of cells and connective tissue proteins. Human gingival fibroblasts were cultured with cyclosporine A, and the expression of two vital endoplasmic stress markers and two prime matrix proteins (connective tissue growth factor (CTGF and periostin) were assessed by RT-PCR. We found that expression of Glucose-Regulated Protein 78 (GRP78/BIP) and CCAAT/enhancer binding protein, C/EBP homologous protein (CHOP) were significantly increased, along with CTGF and periostin, suggesting a role for these factors in gingival overgrowth.

Subject(s)

Cell Adhesion Molecules/genetics , Connective Tissue Growth Factor/genetics , Cyclosporine/adverse effects , Endoplasmic Reticulum Stress/drug effects , Heat-Shock Proteins/genetics , Immunosuppressive Agents/adverse effects , Transcription Factor CHOP/genetics , Biomarkers/metabolism , Cell Adhesion Molecules/agonists , Cell Adhesion Molecules/metabolism , Connective Tissue Growth Factor/agonists , Connective Tissue Growth Factor/metabolism , Endoplasmic Reticulum/drug effects , Endoplasmic Reticulum/metabolism , Endoplasmic Reticulum Chaperone BiP , Endoplasmic Reticulum Stress/genetics , Fibroblasts/cytology , Fibroblasts/drug effects , Fibroblasts/metabolism , Gene Expression Regulation , Gingiva/cytology , Gingiva/drug effects , Gingiva/metabolism , Heat-Shock Proteins/agonists , Heat-Shock Proteins/metabolism , Humans , Primary Cell Culture , Signal Transduction , Transcription Factor CHOP/agonists , Transcription Factor CHOP/metabolism

ABSTRACT

Subject(s)

SEND TO:

SELECTION OF CITATIONS

SEARCH DETAIL