ABSTRACT
Influence of synthetic peptides identical to fragments of natural human immunodeficiency virus (HIV) glycoproteins gp 120 and gp 41, on luminol-enhanced chemiluminescence (CL) of human neutrophils has been studied. It was established that some of peptide analogs of gp 120 and gp 41 immunodominant regions are able to suppress spontaneous CL: but when being used with dimethylsulfoxide they dramatically stimulate it and deteriorate opsonized zymosan-induced CL. Conclusions about the necessity of possible side effects considering during use of peptide vaccines against HIV have been made. It is also possible to explain some neutrophil dysfunction in HIV infected subjects as the result of HIV glycoproteins direct influence on this cells.
Subject(s)
HIV Envelope Protein gp120/chemistry , HIV Envelope Protein gp41/chemistry , Immunodominant Epitopes/immunology , Neutrophils/metabolism , Oxygen/metabolism , Acquired Immunodeficiency Syndrome/metabolism , Acquired Immunodeficiency Syndrome/pathology , Dimethyl Sulfoxide/pharmacology , Humans , Luminescent Measurements , Neutrophils/drug effects , Neutrophils/immunology , Respiratory Burst , Zymosan/pharmacologyABSTRACT
It was found out that macrophage superoxide production is stimulated by intracellular contacts. The high level of the nitro-blue tetrazolium salt reduction in individual cells non-contacting with others is reached when using fibronectin or serum-opsonized zymosan but not phorbol myristate acetate or chemotactic formyl-peptide as activators. The reaction enhancement in non-contacting macrophages stimulated with phorbol ester occurs in the presence of Arg-Gly-Asp-Ser synthetic peptide.
Subject(s)
Cell Communication , Macrophages/drug effects , Amino Acid Sequence , Animals , Arginine , Aspartic Acid , Fibronectins/pharmacology , Glycine , Male , Mice , Mice, Inbred C57BL , Tetradecanoylphorbol Acetate/pharmacology , Zymosan/pharmacologySubject(s)
Appendix/anatomy & histology , Lymphoid Tissue/anatomy & histology , Adult , Animals , Animals, Newborn/immunology , Appendix/cytology , Appendix/immunology , B-Lymphocytes/immunology , Cell Count , Humans , Immunoglobulins/biosynthesis , Immunologic Surveillance , Lymphocytes/cytology , Male , Neoplasms, Experimental/immunology , Rabbits , Receptors, Antigen, B-Cell/analysis , T-Lymphocytes/immunologySubject(s)
Neoplasms/prevention & control , Retinoids/therapeutic use , Vitamin A/therapeutic use , Adjuvants, Immunologic/therapeutic use , Animals , Breast Neoplasms/prevention & control , Carcinogens , Cricetinae , Diterpenes , Female , Humans , Keratoacanthoma/drug therapy , Lung Neoplasms/prevention & control , Male , Mice , Neoplasms/chemically induced , Prostatic Neoplasms/prevention & control , Rats , Retinoids/immunology , Retinyl Esters , Tretinoin/therapeutic use , Vitamin A/analogs & derivatives , Vitamin A/immunologyABSTRACT
The effect of appendectomy on carcinoma RS-1 growth has been studied in young and old Wistar rats and in randombred ones. It has been found that the tumor grew more intensely in the appendectomized animals. This effect was evident in the young animals, both linear and randombred ones. It remained unchanged after administration of the retinoids (all-transmethylretinoate and retinoid C15) but did not develop in the old animals.
