ABSTRACT
The neutral lipid content of Human Amnion cells in tissue culture, studied by cytophotometry, increases after irradiation with 60Co gamma-rays. Cells having an already elevated lipid content, induced by lipid pretreatments, become sensitized to irradiation as determined by cell survival and electrophoretic mobility studies.
Subject(s)
Amnion/radiation effects , Cell Survival/radiation effects , Lipids/radiation effects , Amnion/chemistry , Amnion/cytology , Azo Compounds , Cells, Cultured , Cytophotometry , Electrophoresis , Gamma Rays , Humans , Lipids/pharmacology , Naphthalenes , Radiation Tolerance/drug effects , Staining and LabelingABSTRACT
The selective staining of neutral lipids in Human Amnion cells in tissue culture was achieved using a particular fraction of the lipid dye, Sudan black B and a standardized staining procedure. The fraction, termed SBB-I, was isolated by thin-layer chromatography. The cytophotometric assessment of intracellular neutral lipids, stained with SBB-I, is described and applied to the study of changes in granulocytic neutral lipids in leukemia.
