ABSTRACT
Owing to the weak reactivities of monomeric DManα1 and Galß1-->3/4GlcNAcß (I(ß)/II(ß)) glycotopes with Ralstonia solanacearum lectin (RSL), their recognition roles were previously ignored. In this study, the interaction intensities of RSL toward four monomeric glycotopes LFucα1-->, DManα1--> and I(ß)/II(ß) within two combining sites were established by both enzyme-linked lectinosorbent and inhibition assays. It was found that high density of LFucα1--> complex enhanced the recognition intensities at LFucα1--> site, polyvalent DManα1--> was essential for binding at the DManα1--> site and polyvalent I(ß)/II(ß) was required at LFucα1--> site. The peculiar recognition systems of RSL are very different from other well known microbial lectins.
Subject(s)
Acetylglucosamine/chemistry , Acetylglucosamine/metabolism , Ralstonia solanacearum , Receptors, N-Acetylglucosamine/metabolism , Animals , Binding Sites , Carbohydrate Sequence , Glycoproteins/metabolism , Mannans/metabolism , Molecular Sequence Data , Protein BindingSubject(s)
Bacterial Proteins/chemistry , Epitopes/chemistry , Glycoconjugates/chemistry , Lectins/chemistry , Monosaccharides/chemistry , Oligosaccharides/chemistry , Animals , Archaea , Bacteria , Bacterial Proteins/metabolism , Binding Sites , Birds , Body Fluids/chemistry , Body Fluids/metabolism , Carbohydrate Conformation , Carbohydrate Sequence , Crystallography, X-Ray , Epitopes/metabolism , Erythrocytes/chemistry , Erythrocytes/metabolism , Glycoconjugates/metabolism , Hemagglutination Inhibition Tests , Humans , Lectins/metabolism , Mammals , Models, Molecular , Molecular Sequence Data , Monosaccharides/metabolism , Oligosaccharides/metabolism , Protein BindingSubject(s)
Adhesins, Bacterial/biosynthesis , Bacterial Proteins/biosynthesis , Chromobacterium/chemistry , Lectins/biosynthesis , Pseudomonas aeruginosa/chemistry , Adhesins, Bacterial/chemistry , Adhesins, Bacterial/genetics , Amino Acid Sequence , Animals , Bacterial Adhesion/drug effects , Bacterial Proteins/chemistry , Bacterial Proteins/genetics , Choline/pharmacology , Chromobacterium/drug effects , Chromobacterium/metabolism , Chromobacterium/pathogenicity , Erythrocytes , Erythromycin/pharmacology , Ethanol/pharmacology , Glycoconjugates/chemistry , Glycoconjugates/metabolism , Hemagglutination Inhibition Tests , Humans , Lectins/chemistry , Lectins/genetics , Mice , Molecular Sequence Data , Monosaccharides/chemistry , Monosaccharides/metabolism , Oligosaccharides/chemistry , Oligosaccharides/metabolism , Pseudomonas aeruginosa/drug effects , Pseudomonas aeruginosa/metabolism , Pseudomonas aeruginosa/pathogenicity , Quorum Sensing/drug effects , Trehalose/pharmacology , Virulence Factors/biosynthesis , Virulence Factors/chemistryABSTRACT
Ralstonia solanacearum lectin (RSL), that might be involved in phytopathogenicity, has been defined as LFuc>>Man specific. However, the effects of polyvalency of glycotopes and mammalian structural units on binding have not been established. In this study, recognition factors of RSL were comprehensively examined with natural multivalent glycotopes and monomeric ligands using enzyme linked lectin-sorbent and inhibition assays. Among the glycans tested, RSL reacted strongly with multivalent blood group A(h) (GalNAcalpha1-3[Fucalpha1-2]Gal) and H (Fucalpha1-2Gal) active glycotopes, followed by B(h) (Galalpha1-3[Fucalpha1-2]Gal), Le(a) (Galbeta1-3[Fucalpha1-4]GlcNAc) and Le(b) (Fucalpha1-2Galbeta1-3[Fucalpha1-4]GlcNAc) active glycotopes. But weak or negligible binding was observed for blood group precursors having Galbeta1-3/4GlcNAcbeta1- (Ibeta/IIbeta) residues or Galbeta1-3GalNAcalpha1- (Talpha), GalNAcalpha1-Ser/Thr (Tn) bearing glycoproteins. These results indicate that the density and degree of exposure of multivalent ligands of alpha1-2 linked LFuc to Gal at the non-reducing end is the most critical factor for binding. An inhibition study with monomeric ligands revealed that the combining site of RSL should be of a groove type to fit trisaccharide binding with highest complementarity to blood group H trisaccharide (H(L); Fucalpha1-2Galbeta1-4Glc). The outstandingly broad RSL saccharide-binding profile might be related to the unusually wide spectrum of plants that suffer from R. solanacearum pathogenicity and provide ideas for protective antiadhesion strategies.
Subject(s)
ABO Blood-Group System/chemistry , Lewis Blood Group Antigens/chemistry , Plant Lectins/chemistry , ABO Blood-Group System/metabolism , Animals , Carbohydrate Sequence , Disaccharides/chemistry , Humans , Molecular Sequence Data , Mucins/chemistry , Swine , Trisaccharides/chemistryABSTRACT
Chromobacterium violaceum is a versatile, violet pigment (violacein)-producing beta-proteobacterium, confined to tropical and subtropical regions, dwelling in soil and water, like Pseudomonas aeruginosa and Ralstonia solanacearum. These three bacteria are saprophytes that occasionally become aggressive opportunistic pathogens virulently attacking animals (the first two) and plants (the third). The recent availability of their genome sequences enabled identification in the C. violaceum genome of an ORF (locus no. 1744) that is similar to those of P. aeruginosa and R. solanacearum lectins, PA-IIL and RS-IIL, respectively. A recombinant protein, CV-IIL, encoded by that ORF exhibited fucose>mannose-specific lectin activity resembling PA-IIL. This paper describes production and properties of the native CV-IIL, which, like PA-IIL and RS-IIL, is probably also a quorum-sensing-driven secondary metabolite, appearing concomitantly with violacein. Its formation is repressed in the CV026 mutant of C. violaceum, which lacks endogenous N-acylhomoserine lactone. The upstream extragenic sequence of its ORF contains a 20 bp sequence (5'-101-120) with partial similarities to the luxI-box and the related P. aeruginosa and R. solanacearum promoter boxes of quorum-sensing-controlled genes. The lectin level is augmented by addition of trehalose to the medium. The subunit size of CV-IIL (around 11.86 kDa) is similar to those of PA-IIL (11.73 kDa) and RS-IIL (11.60 kDa). Like PA-IIL, in the tetrameric form CV-IIL preferentially agglutinates alpha1-2 fucosylated H-positive human erythrocytes (regardless of their A, B or O type), as opposed to the O(h) Bombay type, but differs from it in having no interaction with rabbit erythrocytes and in displaying stronger affinity to l-galactose than to l-fucose. The greater similarity of CV-IIL to PA-IIL than to RS-IIL might be related to the selective adaptation of both C. violaceum and P. aeruginosa to animal tissues versus the preferential homing of R. solanacearum to plants.
Subject(s)
Bacterial Proteins/chemistry , Chromobacterium/metabolism , Lectins/metabolism , Pseudomonas aeruginosa/metabolism , Ralstonia solanacearum/metabolism , Bacterial Proteins/genetics , Bacterial Proteins/metabolism , Chromobacterium/chemistry , Fucose/metabolism , Lectins/chemistry , Lectins/genetics , Protein BindingABSTRACT
The plant pathogen Ralstonia solanacearum produces two lectins, each with different affinity to fucose. We described previously the properties and sequence of the first lectin, RSL (subunit M(r) 9.9 kDa), which is related to fungal lectins (Sudakevitz, D., Imberty, A., and Gilboa-Garber, N., 2002, J Biochem 132: 353-358). The present communication reports the discovery of the second one, RS-IIL (subunit M(r) 11.6 kDa), a tetrameric lectin, with high sequence similarity to the fucose-binding lectin PA-IIL of Pseudomonas aeruginosa. RS-IIL recognizes fucose but displays much higher affinity to mannose and fructose, which is opposite to the preference spectrum of PA-IIL. Determination of the crystal structure of RS-IIL complexed with a mannose derivative demonstrates a tetrameric structure very similar to the recently solved PA-IIL structure (Mitchell, E., et al., 2002, Nature Struct Biol 9: 918-921). Each monomer contains two close calcium cations that mediate the binding of the monosaccharide and explain the outstandingly high affinity to the monosaccharide ligand. The binding loop of the cations is fully conserved in RS-IIL and PA-IIL, whereas the preference for mannose versus fucose can be attributed to the change of a three-amino-acid sequence in the 'specificity loop'.
Subject(s)
Adhesins, Bacterial/chemistry , Bacterial Proteins/chemistry , Lectins/chemistry , Mannose-Binding Lectin/chemistry , Protein Structure, Tertiary , Pseudomonas aeruginosa/chemistry , Ralstonia solanacearum/chemistry , Adhesins, Bacterial/genetics , Adhesins, Bacterial/metabolism , Amino Acid Sequence , Bacterial Proteins/genetics , Bacterial Proteins/metabolism , Binding Sites , Calcium/metabolism , Chelating Agents/metabolism , Crystallography, X-Ray , Edetic Acid/metabolism , Lectins/genetics , Lectins/metabolism , Mannose-Binding Lectin/genetics , Mannose-Binding Lectin/metabolism , Mannosides/chemistry , Mannosides/metabolism , Methylmannosides , Models, Molecular , Molecular Sequence Data , Molecular Structure , Monosaccharides/chemistry , Monosaccharides/metabolism , Sequence AlignmentABSTRACT
The structure of the tetrameric Pseudomonas aeruginosa lectin I (PA-IL) in complex with galactose and calcium was determined at 1.6 A resolution, and the native protein was solved at 2.4 A resolution. Each monomer adopts a beta-sandwich fold with ligand binding site at the apex. All galactose hydroxyl groups, except O1, are involved in a hydrogen bond network with the protein and O3 and O4 also participate in the co-ordination of the calcium ion. The stereochemistry of calcium galactose binding is reminiscent of that observed in some animal C-type lectins. The structure of the complex provides a framework for future design of anti-bacterial compounds.
Subject(s)
Adhesins, Bacterial/metabolism , Calcium/metabolism , Galactose/metabolism , Lectins/metabolism , Pseudomonas aeruginosa/metabolism , Adhesins, Bacterial/chemistry , Binding Sites , Calcium/chemistry , Crystallography, X-Ray , Galactose/chemistry , Hydrogen Bonding , Lectins/chemistry , Models, Molecular , Protein Binding , Protein ConformationABSTRACT
Pseudomonas aeruginosa galactose- and fucose-binding lectins (PA-IL and PA-IIL) contribute to the virulence of this pathogenic bacterium, which is a major cause of morbidity and mortality in cystic fibrosis patients. The crystal structure of PA-IIL in complex with fucose reveals a tetrameric structure. Each monomer displays a nine-stranded, antiparallel b-sandwich arrangement and contains two close calcium cations that mediate the binding of fucose in a recognition mode unique among carbohydrate-protein interactions. Experimental binding studies, together with theoretical docking of fucose-containing oligosaccharides, are consistent with the assumption that antigens of the Lewis a (Le(a)) series may be the preferred ligands of this lectin. Precise knowledge of the lectin-binding site should allow a better design of new antibacterial-adhesion prophylactics.
Subject(s)
Adhesins, Bacterial/chemistry , Bacterial Adhesion , Fucose/chemistry , Lectins/chemistry , Models, Molecular , Pseudomonas aeruginosa/pathogenicity , Adhesins, Bacterial/metabolism , Binding Sites , Calcium/chemistry , Calcium/metabolism , Crystallography, X-Ray , Cystic Fibrosis/microbiology , Fucose/metabolism , Humans , Lectins/metabolism , Lung/microbiology , Macromolecular Substances , Oligosaccharides/chemistry , Oligosaccharides/metabolism , Protein BindingABSTRACT
The worldwide distributed plant aggressive pathogen Ralstonia solanacearum, which causes lethal wilt in many agricultural crops, produces a potent L-fucose-binding lectin (RSL) exhibiting sugar specificity similar to that of PA-IIL of the human aggressive opportunistic pathogen Pseudomonas aeruginosa. Both lectins show L-fucose > L-galactose > D-arabinose > D-mannose specificity, but the affinities of RSL to these sugars are substantially lower. Unlike Ulex europaeus anti-H lectin, but like PA-IIL and Aleuria aurantia lectin (AAL), RSL agglutinates H-positive human erythrocytes regardless of their type, O, A, B, or AB, and animal erythrocytes (papain-treated ones more strongly than untreated ones). It also interacts with H and Lewis chains in the saliva of "secretors" and "nonsecretors." RSL purification is easier than that of PA-IIL since R. solanacearum extracts do not contain a galactophilic PA-IL-like activity. Mass spectrometry and 35 N-terminal amino acid sequencing enabled identification of the RSL protein (subunit approximately 9.9 kDa, approximately 90 amino acids) in the complete genome sequence of this bacterium. Despite the greater phylogenetic proximity of R. solanacearum to P. aeruginosa, and the presence of a PA-IIL-like gene in its genome, the RSL structure is not related to that of PA-IIL, but to that of the fucose-binding lectin of the mushroom (fungus) Aleuria aurantia, which like the two bacteria is a soil inhabitant.