Computational Models-Based Detection of Peripheral Malarial Parasites in Blood Smears.

The most common human parasite as per the medical experts is the malarial disease, which is caused by a protozoan parasite, and Plasmodium falciparum, a common parasite in humans. A microscopist with expertise in malaria diagnosis must conduct this complex procedure to identify the stages of infection. This epidemic is an ongoing disease in some parts of the world, which is commonly found. A Kaggle repository was used to upload the data collected from the NIH portal. The dataset contains 27558 samples, of which 13779 samples carry parasites and 13779 samples do not. This paper focuses on two of the most common deep transfer learning methods. Unlike other feature extractors, VGG-19's fine-tuning and pretraining made it an ideal feature extractor. Several image classification models, including VGG-19, have been pretrained on larger datasets. Additionally, deep learning strategies based on pretrained models are proposed for detecting malarial parasite cases in the early stages, in addition to an accuracy rating of 98.34 ∗ 0.51%.

Molecular docking, QSAR properties and DNA/BSA binding, anti-proliferative studies of 6-methoxy benzothiozole imine base and its metal complexes.

The molecular and QSAR (Quantitative Structure-Activity Relationship) properties of title compound 2-((6-Methoxybenzo[d]thiazol-2-ylimino)methyl)-6-ethoxyphenol (HL) were evaluated employing HyperChem 7.5 tools. The interaction of the 1a-1e complexes of HL with calf thymus DNA (CT-DNA) was investigated by absorption titrations, Fluorescence quenching and viscosity measurements. The experimental data suggest that these complexes bind to CT-DNA through an intercalative mode, wherein DNA-binding affinity of 1e is found to be greater compared to other complexes. The tryptophan emission-quenching with bovine serum albumin (BSA) experiment revealed stronger binding of 1e than other complexes in the hydrophobic region of protein. The photocleavage of plasmid pBR322 DNA investigated in the presence of the title complexes inferred conversion of supercoiled form of DNA plasmid to circular nicked form. Free-radical scavenging activity studies of HL and its metal complexes determined by their interaction with the stable free-radical DPPH have shown promising antioxidant property. Further cytotoxicity studies with HeLa and MCF-7 cell lines indicated that the compounds can efficiently inhibit the cell proliferation in a dose dependent manner. The DAPI staining assay studies revealed the higher potency of 1e to induce apoptosis. AbbreviationsBSABovine serum albumin proteinCT-DNACalf thymus DNADMSODimethyl sulfoxideDAPI4',-6-Diamidino-2-phenylindole dihydrochlorideESI-MSElectrospray ionization mass spectrometryIC50Half-maximal inhibitory concentrationMBTYE2-((6-methoxybenzo[d]thiazol-2-ylimino) methyl)-6-ethoxyphenolMTT3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromidePBSPhosphate-buffered salineTrisTris(hydroxymethyl)aminomethaneCommunicated by Ramaswamy H. Sarma.