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1.
Org Biomol Chem ; 13(21): 5862-6, 2015 Jun 07.
Article in English | MEDLINE | ID: mdl-25917375

ABSTRACT

A lactone-fused cyclohexadiene, which can be readily prepared by ruthenium(III)-catalyzed [2 + 2 + 2] cyclization of an enediyne, functioned as a versatile platform for the stereoselective synthesis of differently functionalized 5,6,5-tricyclic scaffolds.


Subject(s)
Cyclohexenes/chemical synthesis , Lactones/chemical synthesis , Catalysis , Cyclization , Cyclohexenes/chemistry , Lactones/chemistry , Models, Molecular , Ruthenium/chemistry , Stereoisomerism
2.
Res Commun Mol Pathol Pharmacol ; 110(5-6): 319-32, 2001.
Article in English | MEDLINE | ID: mdl-12889524

ABSTRACT

Antimetabolites such as methotrexete and 6-mercaptopurine have been shown to have circadian variations in their toxicities. However, chronopharmacological profiles of mizoribine (Miz) that is newly synthesized as an anti-metabolic agent for immunosuppression, have not been evaluated. In this study, we examined the dosing time-dependent alterations in the pharmacokinetics and pharmacodynamics of Miz. In addition, chronopharmacology of azathiopurine (Aza) was also evaluated to compare with that of Miz. Initially, Miz (10 and 20 mg/kg) or Aza (20 mg/kg) was orally administered at 8:00 hr or 20:00 hr for 3 weeks to rats. To reveal the dosing time-dependent difference of pharmacokinetics, Miz (20 mg/kg) was orally given at 8:00 hr or 20:00 hr and blood was obtained for 12 hours. Finally, Miz (20 mg/kg) or Aza (20 mg/kg) was administered at 8:00 hr or 20:00 hr to rats with heterotopic allogeneic heart grafts. The Miz group treated at 8:00 hr and Aza group treated at 20:00 hr showed severe myelosuppression compared with their each opposite dosing time. AUC of Miz in the morning trial was twice as high as that in the evening trial. The graft survival durations of the Miz- and Aza-treated groups were significantly longer than those of the respective control groups, but were not affected by dosing time of each agent. These results suggest that the toxicity, but not efficacy of Miz is varied with the dosing time. The chronotoxicological phenomenon of Miz might be, at least in part, explained by the dosing time-dependent difference in serum drug concentrations and apparent clearance.


Subject(s)
Heart Transplantation , Immunosuppressive Agents , Ribonucleosides , Animals , Area Under Curve , Azathioprine/administration & dosage , Azathioprine/toxicity , Body Weight/drug effects , Dose-Response Relationship, Drug , Drug Administration Schedule , Graft Survival , Half-Life , Immunosuppressive Agents/administration & dosage , Immunosuppressive Agents/pharmacokinetics , Immunosuppressive Agents/pharmacology , Male , Rats , Rats, Inbred Lew , Ribonucleosides/administration & dosage , Ribonucleosides/pharmacokinetics , Ribonucleosides/pharmacology
3.
J Inorg Biochem ; 68(1): 39-44, 1997 Oct.
Article in English | MEDLINE | ID: mdl-9379179

ABSTRACT

A neutral, Ag(I)-N bonding compound, polymeric silver(I)-imidazolate [Ag(imd)]n (1) consisting of Ag+: imd = 1:1 (Himd = imidazole, C3H4N2), showed wide spectra in effective antimicrobial activities against bacteria, yeast and mold. Of particular note are the activities against a wide range of mold. This polymeric solid does not crystallize and is sparingly soluble in all solvents. The monomeric, cationic, water-soluble Ag(I)-N bonding complex, [Ag(Himd)2](NO3) (2), has also shown wide spectra of effective antimicrobial activities. These activities observed here were significantly different from those of the recently prepared oligomeric Ag(I)-S bonding complexes; the latter have shown narrow spectra. It is proposed that the Ag(I)-N bonding is one of the key factors showing the wide spectra of antimicrobial activities and the potential targets for inhibition of bacteria and yeast by these Ag(I) complexes are proteins, but not nucleic acids. The physico-chemical properties of (1), in comparison with those of (2), with various measurements (FT-IR, Laser Raman scattering spectroscopy, ESCA and solid 13C CP-MAS NMR spectroscopies) are described.


Subject(s)
Anti-Infective Agents/chemistry , Anti-Infective Agents/pharmacology , Imidazoles/chemistry , Imidazoles/pharmacology , Silver Compounds/pharmacology , Anti-Bacterial Agents , Bacteria/drug effects , Fungi/drug effects , Magnetic Resonance Spectroscopy , Microbial Sensitivity Tests , Organometallic Compounds/chemistry , Organometallic Compounds/pharmacology , Polymers/chemistry , Polymers/pharmacology , Silver Compounds/chemistry , Spectroscopy, Fourier Transform Infrared , Spectrum Analysis, Raman
4.
J Clin Pharmacol ; 36(3): 203-5, 1996 Mar.
Article in English | MEDLINE | ID: mdl-8690813

ABSTRACT

The present study was undertaken to examine the effect of age on diurnal variation in glomerular filtration rate (GFR). The GFR, as estimated by creatinine clearance (Clcr), was determined during a 24-hour period in 10 younger (mean +/- SD age 42 +/- 9 years) and 10 older (mean age 75 +/- 4 years) patients with hypertension. Significant diurnal variations in Clcr were observed in the younger patients, with a peak during the day and trough during the night. Such were not observed in the older patients, however. These results suggest that diurnal variation in GFR is affected by age. Chronopharmacologic profiles of drugs, which are mainly excreted in urine by glomerular filtration, might be altered in these patients.


Subject(s)
Circadian Rhythm , Glomerular Filtration Rate/physiology , Hypertension/physiopathology , Adult , Age Factors , Aged , Creatinine/metabolism , Female , Humans , Male , Middle Aged
5.
Eur J Clin Pharmacol ; 51(1): 95-8, 1996.
Article in English | MEDLINE | ID: mdl-8880059

ABSTRACT

OBJECTIVE: To examine the effect of ranitidine on the renal clearance of lomefloxacin. SETTING: Department of Clinical Pharmacology, Jichi Medical School. METHODS: Lomefloxacin 200 mg and ranitidine 300 mg or its placebo were given orally in a randomised, double-blind, crossover design. Blood and urine samples were obtained during a 24-h period after dosing. RESULTS: The area under the plasma concentration-time curve and the elimination half-life of lomefloxacin were significantly increased following coadministration with ranitidine. These effects were caused by significant decreases in total (7.8%) and renal (22%) clearance of lomefloxacin. In contrast, creatinine clearance and urinary excretion of electrolytes were not influenced by ranitidine. CONCLUSION: As lomefloxacin and ranitidine are excreted in urine by renal tubular secretion, the present results suggest that the renal tubular secretion of lomefloxacin is diminished by ranitidine. As the reduction in lomefloxacin clearance is only marginal, it is probable that the drug interaction observed in this study is not of clinical significance.


Subject(s)
Anti-Infective Agents/pharmacokinetics , Fluoroquinolones , Histamine H2 Antagonists/pharmacology , Quinolones/pharmacokinetics , Ranitidine/pharmacology , Adult , Anti-Infective Agents/blood , Anti-Infective Agents/urine , Cross-Over Studies , Double-Blind Method , Drug Interactions , Drug Therapy, Combination , Humans , Male , Quinolones/blood , Quinolones/urine
6.
Chronobiol Int ; 11(2): 113-8, 1994 Apr.
Article in English | MEDLINE | ID: mdl-8033239

ABSTRACT

The influence of age on the time-dependent difference in urinary excretion of furosemide, a loop diuretic agent, was examined in this longitudinal study. Male Wistar rats were maintained under conditions of light from 07:00 to 19:00 h and dark from 19:00 to 07:00 h. Furosemide (30 mg/kg) was given orally at 12:00 h (day trial) or 00:00 h (night trial) to rats at 3 months of age, and urine was collected for 8 h after dosage. Thereafter, the identical protocol was repeated using the same animals at 6, 9, 12, 15, 18, and 21 months of age. The urinary excretion of furosemide was significantly greater in the day than in the night trial at 3 months of age. Such a time-dependent difference was observed for up to 15 months, but disappeared at 18 and 21 months of age. The time-dependent difference in urinary excretion of furosemide (day trial - night trial) decreased gradually throughout the observation period of the study. These results suggest that the time-dependent difference in the urinary excretion of furosemide diminishes during the aging process and disappears by 18 months of age in male Wistar rats.


Subject(s)
Aging/physiology , Circadian Rhythm , Furosemide/urine , Administration, Oral , Aging/urine , Animals , Drug Administration Schedule , Furosemide/administration & dosage , Furosemide/pharmacokinetics , Male , Rats , Rats, Wistar
7.
Life Sci ; 55(9): 707-11, 1994.
Article in English | MEDLINE | ID: mdl-8065233

ABSTRACT

The present study was undertaken to examine an effect of an angiotensin II type 1 (AT1) receptor antagonist on urinary prostaglandin E2 (PGE2) excretion following furosemide, a loop diuretic, in rats. Furosemide (30 mg/kg) was given orally with or without pretreatment with derapril (30 mg/kg), an angiotensin converting enzyme inhibitor, TCV-116 (1 mg/kg), an AT1 receptor antagonist, or losartan (10 mg/kg), another AT1 receptor antagonist. The 6-hour urine was collected following furosemide, and the urinary excretion of PGE2 was determined. The urinary PGE2 increased significantly following furosemide alone. However, such a furosemide-induced increase was not observed with pretreatment with derapril, TCV-116 or losartan. These results suggest that the increased urinary excretion of PGE2 following furosemide is blunted by derapril, TCV-116 and losartan. As TCV-116 and losartan are selective AT1 receptor antagonists, the effect of furosemide on renal PGE2 production, as reflected by the urinary PGE2, might be mediated by an activation of AT1 receptors.


Subject(s)
Angiotensin Receptor Antagonists , Benzimidazoles/pharmacology , Biphenyl Compounds/pharmacology , Dinoprostone/urine , Furosemide/pharmacology , Imidazoles/pharmacology , Indans/pharmacology , Prodrugs/pharmacology , Tetrazoles/pharmacology , Angiotensin II/antagonists & inhibitors , Angiotensin-Converting Enzyme Inhibitors/pharmacology , Animals , Losartan , Male , Rats , Rats, Wistar
8.
Eur J Clin Pharmacol ; 46(3): 267-9, 1994.
Article in English | MEDLINE | ID: mdl-8070509

ABSTRACT

The interaction between lomefloxacin, a new quinolone, and furosemide, a loop diuretic, has been examined. Oral lomefloxacin 200 mg and furosemide 40 mg were given together or separately to 8 healthy subjects, and blood and urine samples were obtained over the following 12 h. The plasma concentrations of lomefloxacin following coadministration with furosemide were higher than after lomefloxacin alone and its AUC was increased, and its total and renal clearances were decreased. No change in the pharmacokinetics of furosemide was found after coadministration of lomefloxacin. As quinolones and furosemide are reported to be excreted in urine by the renal tubular anion transport system, the present results suggest that the renal tubular secretion of lomefloxacin is diminished by furosemide. It is not clear whether this pharmacokinetic interaction might be clinically important.


Subject(s)
Anti-Infective Agents/pharmacokinetics , Fluoroquinolones , Furosemide/pharmacology , Quinolones/pharmacokinetics , Adult , Anti-Infective Agents/urine , Chlorides/urine , Double-Blind Method , Drug Interactions , Furosemide/blood , Furosemide/pharmacokinetics , Humans , Male , Potassium/urine , Quinolones/urine , Sodium/urine , Urodynamics/drug effects
9.
Life Sci ; 55(5): 367-72, 1994.
Article in English | MEDLINE | ID: mdl-8035650

ABSTRACT

The present study was undertaken to examine whether there was a time-dependent change in the toxic effects of amikacin, an aminoglycoside, on renal functions. Male Wistar rats were maintained under conditions of light from 7 am to 7 pm and dark from 7 pm to 7 am. Amikacin (1.2 g/kg) was injected intraperitoneally to animals at 4 am, 10 am, 4 pm or 10 pm. Glomerular function estimated by creatinine clearance (Clcr) and tubular function estimated by urinary excretion of a loop diuretic, furosemide, which was excreted in urine mainly by tubular secretion, were determined before and 24 hours after amikacin injection. The values of these parameters were reduced by amikacin at each observation point. The magnitude of these decrements was greatest at 4 pm both for Clcr and urinary furosemide excretion. These results suggest that the toxic effects of amikacin on renal glomerular and tubular functions vary with its time of administration.


Subject(s)
Amikacin/toxicity , Kidney/drug effects , Amikacin/pharmacokinetics , Animals , Kidney/physiology , Male , Rats , Rats, Wistar , Time Factors
10.
Life Sci ; 55(5): 373-8, 1994.
Article in English | MEDLINE | ID: mdl-8035651

ABSTRACT

We have recently demonstrated that the time-dependent difference in urinary excretion of furosemide, a loop diuretic, diminishes during the aging process and disappears by 18 months of age in rats. The present study was undertaken to examine whether the amplitude of the daily variations in the urinary excretion of furosemide or their pattern, or both, are influenced in aged animals. Young (3 months of age) and aged (30 months of age) Wistar rats were maintained under conditions of light from 7 am to 7 pm and dark from 7 pm to 7 am. Furosemide (30 mg/kg) was given orally at 4 am, 8 am, 12 am, 4 pm, 8 pm or 12 pm. Urine was collected for 8 hours after furosemide administration and urinary excretion of furosemide was determined. There were significant daily variations in the urinary furosemide and the urine volume with the peak at 8 am and the trough at 12 pm in both groups of rats. The differences in these parameters between the 8 am and 12 pm trials were significantly smaller in the aged than in the young rats. These results suggest that the age-related alteration in the time-dependent phenomenon of furosemide is caused by the decreased amplitude of the daily variation in the urinary furosemide excretion and its diuretic effect.


Subject(s)
Furosemide/urine , Age Factors , Animals , Male , Rats , Rats, Wistar , Time Factors
11.
Jpn J Pharmacol ; 62(4): 403-5, 1993 Aug.
Article in English | MEDLINE | ID: mdl-7901444

ABSTRACT

Influence of alpha-receptor blockade on the time-dependent change in the effect of furosemide (a loop diuretic agent) was examined. Furosemide (30 mg/kg) was given orally to the doxazosin (an alpha 1-blocker)- or vehicle-treated rats at 12 AM or 12 PM. Urine volume and urinary excretions of of sodium and furosemide for 8 hr were significantly greater at 12 AM than at 12 PM in the vehicle-treated animals. However, such time-dependent changes in these parameters disappeared in the doxazosin-treated rats. These results suggest that the alpha 1-receptor-mediated stimuli are involved in the mechanism of the time-dependent change in the effect of furosemide.


Subject(s)
Adrenergic alpha-Antagonists/pharmacology , Furosemide/pharmacology , Adrenergic alpha-1 Receptor Antagonists , Adrenergic alpha-Antagonists/pharmacokinetics , Animals , Blood Pressure/drug effects , Chromatography, High Pressure Liquid , Doxazosin/pharmacology , Furosemide/pharmacokinetics , Male , Rats , Rats, Wistar , Sodium/urine , Time Factors
12.
J Clin Pharmacol ; 33(7): 640-3, 1993 Jul.
Article in English | MEDLINE | ID: mdl-8396158

ABSTRACT

It has been reported that the urinary excretions of chloride (Cl), potassium (K), and magnesium (Mg), but not sodium (Na), after furosemide, a loop diuretic, were decreased by pretreatment with lisinopril, an ACE inhibitor in hypertensive subjects. The electrolytes disturbance induced by furosemide might be ameliorated by lisinopril. The present study re-examines this potential drug interaction in healthy subjects. Lisinopril (20 mg) or its matching placebo was given orally using a double-blind, crossover design. Four hours after lisinopril administration, furosemide (20 mg) was injected intravenously and urine was collected during the following intervals: 0-0.5, 0.5-1, 1-1.5, 1.5-2, 2-3, 3-4, and 4-6 hours. Blood samples for plasma furosemide concentration were obtained at 0.5, 1, 1.5, 2, 3, 4, and 6 hours after the agent. There were no significant differences between the two trials in plasma concentrations of furosemide or urinary excretions of the agent. Urine volume and urinary excretions of electrolytes (Na, Cl, K, and Mg) after the furosemide with lisinopril administration were not significantly different from those of placebo at any observation period. These results suggest that the urinary excretions of electrolytes after furosemide administration are not influenced by pretreatment with lisinopril.


Subject(s)
Angiotensin-Converting Enzyme Inhibitors/pharmacology , Dipeptides/pharmacology , Electrolytes/urine , Furosemide/antagonists & inhibitors , Adult , Double-Blind Method , Drug Interactions , Furosemide/pharmacokinetics , Furosemide/pharmacology , Humans , Lisinopril , Male , Premedication , Renin/blood
13.
Life Sci ; 52(9): 819-24, 1993.
Article in English | MEDLINE | ID: mdl-8437511

ABSTRACT

Circadian variations in the adrenergic nervous system have been reported to be altered by chronic treatment with clorgyline, a monoamine-oxidase inhibitor. In the present study, the influence of clorgyline on the chronopharmacology of furosemide, a loop diuretic agent, was examined in rats maintained under conditions of light from 7 am to 7 pm and dark from 7 pm to 7 am. Clorgyline (4 mg/kg/day) or its vehicle alone was infused subcutaneously by osmotic minipumps for 14 days. Furosemide (30 mg/kg) was given orally at 12 am [noon (N)] or 12 pm [midnight (M)]. Urine was collected for 8 hours after the agent, and urinary excretions of sodium and furosemide were determined. Urine volume and urinary excretions of sodium and furosemide were significantly greater at 12 N than at 12 M in the vehicle-infused group of rats. However these administration time-dependent changes in the effects of furosemide and its urinary excretion disappeared in the clorgyline-infused animals. These results suggest that the mode of the diurnal variation in the effects of furosemide is altered by chronic treatment with clorgyline. As chronic clorgyline is considered to disturb the adrenergic nervous system, the present findings are compatible with the hypothesis that this system is involved in the mechanism responsible for the time-dependent change in the effects of furosemide.


Subject(s)
Clorgyline/pharmacology , Furosemide/pharmacology , Analysis of Variance , Animals , Circadian Rhythm/drug effects , Clorgyline/administration & dosage , Diuresis/drug effects , Drug Interactions , Furosemide/urine , Infusion Pumps, Implantable , Male , Natriuresis/drug effects , Rats , Rats, Wistar , Sympathetic Nervous System/drug effects
14.
Jpn J Pharmacol ; 60(4): 323-6, 1992 Dec.
Article in English | MEDLINE | ID: mdl-1287267

ABSTRACT

Daily variation in the effects of furosemide, a loop diuretic agent, was examined in Wistar rats maintained under conditions of light from 7 a.m. to 7 p.m. and dark from 7 p.m. to 7 a.m. Furosemide (30 mg/kg) was given orally at 12 p.m., 4 a.m., 8 a.m., 12 a.m., 4 p.m. or 8 p.m. Urine was collected for 8 hr after furosemide administration, and urinary excretions of sodium and furosemide were determined. There were significant daily variations in the urine volume and urinary excretions of sodium and furosemide with a peak at 8 a.m. and a trough at 12 p.m. Significant correlations were observed between the urinary amount of furosemide and its diuretic effects (urine volume and urinary sodium excretion). These results suggest that the diuretic effects of furosemide show daily variations which are, at least in part, caused by the daily variation in the urinary excretion of furosemide.


Subject(s)
Diuretics/pharmacology , Furosemide/pharmacology , Animals , Circadian Rhythm/physiology , Furosemide/pharmacokinetics , Furosemide/urine , Male , Rats , Rats, Wistar , Sodium/urine
15.
Jpn J Pharmacol ; 59(2): 209-12, 1992 Jun.
Article in English | MEDLINE | ID: mdl-1434117

ABSTRACT

We have previously found that the administration-time-dependent change in the effects of furosemide, a loop diuretic agent, is observed in normal rats. The present study was undertaken to examine whether an alteration in this phenomenon occurs in rats with DOCA-saline hypertension. Unilateral nephrectomized rats were divided into three groups. The first group (DOCA-saline) received a 50 mg DOCA tablet intraperitoneally and drank 1% NaCl solution. The other two groups were given sham operations. A 1% NaCl solution was given as drinking water to the second group (control-saline), while tap water was given to the third group (control-water). Furosemide (30 mg/kg) was given orally to each group at 12 a.m. or 12 p.m. Urine was collected for 8 hours after the agent, and urinary excretion of sodium and furosemide were determined. Urine volume and urinary excretion of sodium and furosemide following the agent were significantly greater at 12 a.m. than at 12 p.m. in the control-water and control-saline groups. However, the administration-time-dependent changes in these parameters disappeared in the DOCA-saline rats. These results suggest that the mode of the administration-time-dependent changes in the effects of furosemide is altered in the DOCA-saline hypertensive rats.


Subject(s)
Diuresis/drug effects , Furosemide/administration & dosage , Hypertension/drug therapy , Animals , Circadian Rhythm , Desoxycorticosterone/toxicity , Drug Administration Schedule , Hypertension/chemically induced , Hypertension/physiopathology , Male , Rats , Rats, Wistar , Sodium Chloride/administration & dosage
16.
Life Sci ; 51(19): 1501-7, 1992.
Article in English | MEDLINE | ID: mdl-1435059

ABSTRACT

To examine the influence of mercuric chloride (HgCl2)-induced acute renal damage on urinary excretion of furosemide, HgCl2 (1 mg/kg) or its vehicle alone was given intraperitoneally to Wistar rats. The following two experiments were done. Study I: Three percent body weight (b.w.) of 1% NaCl solution or furosemide (30 mg/kg) in 3% b.w. of 1% NaCl solution was given orally before and after HgCl2 treatment, and an 8-hour urine was collected. Study II: Furosemide (30 mg/kg) was given orally, and blood samples were obtained at 1, 2, 3, 4, 6 and 8 hours after administration. Urinary excretion of N-acetyl-beta-D-glucosaminidase increased, and urine volume and urinary excretions of furosemide and sodium decreased in the HgCl2-treated rats. There were significant correlations between the urinary furosemide and its diuretic effects. Regression lines after HgCl2 were significantly different from those before treatment. The values of absorption as well as elimination rate constant were smaller, while the time to maximum concentration and the elimination half-life were longer in the HgCl2-treated rats compared to vehicle-treated animals. These results suggest that the urinary excretion of furosemide and the responsiveness of renal tubular cells to this agent are impaired in rats with HgCl2-induced acute renal damage.


Subject(s)
Acute Kidney Injury/urine , Furosemide/urine , Mercuric Chloride/poisoning , Acute Kidney Injury/blood , Acute Kidney Injury/chemically induced , Animals , Furosemide/blood , Furosemide/pharmacokinetics , Male , Rats , Rats, Wistar , Regression Analysis , Time Factors
17.
Life Sci ; 51(23): 1811-6, 1992.
Article in English | MEDLINE | ID: mdl-1435089

ABSTRACT

Our previous studies have suggested that the adrenergic nervous system is involved in the mechanism responsible for the time-dependent change in the urinary excretion of furosemide in rats. To examine a potential role of renal nerves in this phenomenon, renal denervation or sham operation was performed using unilaterally nephrectomized rats. Furosemide (30 mg/kg) was given orally at 12 am or 12 pm. Urine was collected for 8 hours after furosemide dosing, and urinary excretions of furosemide and sodium were determined. Urinary furosemide excretion and diuretic effects of the agent (urine volume and urinary sodium) were significantly greater at 12 am than at 12 pm in the sham-operated group of rats. However these administration time-dependent changes in urinary furosemide and its diuretic effects disappeared in the renal-denervated group of animals. These results suggest that the renal nerves contribute to the time-dependent changes in the urinary excretion of furosemide and its subsequent diuretic effects.


Subject(s)
Furosemide/pharmacology , Kidney/drug effects , Kidney/innervation , Administration, Oral , Animals , Chronobiology Phenomena , Denervation , Diuresis/drug effects , Drug Administration Schedule , Furosemide/urine , Male , Nephrectomy , Photoperiod , Random Allocation , Rats , Rats, Wistar , Sodium/urine , Sympathetic Nervous System/physiology
18.
Biochem Biophys Res Commun ; 168(2): 863-70, 1990 Apr 30.
Article in English | MEDLINE | ID: mdl-2139780

ABSTRACT

Two types of natriuretic peptide, atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP), very similar to each other in structure and in pharmacological effect, are known to be present in mammalian heart and brain. In our present survey for unidentified peptides in porcine brain extracts, we found a new peptide of 22 amino acid residues, eliciting a potent relaxant activity on chick rectum. The amino acid sequence determined for the peptide shows remarkable similarity to those of ANP and BNP, especially in the 17-residue sequences flanked by two cysteine residues. The peptide shows a pharmacological spectrum similar to ANP and BNP. Thus, the peptide was designated "C-type natriuretic peptide (CNP)", the third member to join the natriuretic peptide family. In contrast to ANP and BNP, CNP terminates in the second cysteine residue, lacking a further C-terminal extension.


Subject(s)
Atrial Natriuretic Factor/analysis , Brain Chemistry , Nerve Tissue Proteins/analysis , Amino Acid Sequence , Animals , Chickens , Molecular Sequence Data , Natriuretic Peptide, Brain , Natriuretic Peptide, C-Type , Protein Conformation , Rectum/metabolism , Swine
19.
Gan To Kagaku Ryoho ; 17(2): 269-73, 1990 Feb.
Article in Japanese | MEDLINE | ID: mdl-2405778

ABSTRACT

The antitumor activities of CDDP analogs (CBD-CA, NK-121, 254-S) were evaluated preclinically by subrenal capsule assay (SRCA) with cyclophosphamide pretreatment. In the fundamental study, the antitumor activities against serially transplanted human esophageal cancer xenograft (IMEs-1) were compared with subcutaneous transplantation assay in nude mice and SRCA. The antitumor activities in SRCA were similar to those of in nude mice assay system (CBDCA greater than CDDP greater than 254-S greater than NK-121). Thus SRCA was considered to be useful for the evaluation of the activities of these agents. The activities were also tested against 10 human esophageal tumors obtained clinically. The sensitivity rate of these agents were 50% in CDDP, 30% in CBDCA, 30% in NK-121, and 30% in 254-S, respectively. These analogs seemed to be less effective than CDDP. However, in two cases, analogs were active though CDDP were inactive. The results suggest that these analogs are useful for the cases in which CDDP can not be given due to the toxicities and also for outpatient use.


Subject(s)
Antineoplastic Agents/pharmacology , Cisplatin/analogs & derivatives , Esophageal Neoplasms/pathology , Subrenal Capsule Assay , Animals , Carboplatin , Cisplatin/pharmacology , Humans , Mice , Mice, Nude , Organoplatinum Compounds/pharmacology
20.
Neuropeptides ; 15(1): 1-9, 1990 Jan.
Article in English | MEDLINE | ID: mdl-2325835

ABSTRACT

Neuromedin U-25 and its C-terminal octapeptide, neuromedin U-8 are related peptides originally identified in porcine brain which elicit potent uterus stimulant activity. Radioimmunoassay using an antiserum raised against porcine neuromedin U-8, indicated that neuromedin U-like immunoreactivity in the rat was far more abundant in the small intestine than the brain. Neuromedin U in the rat is a single, a 23 amino acid peptide (2, 17). Rat neuromedin U has the same 7 residues on its C-terminus as porcine neuromedin U, and the antiserum against porcine neuromedin U-8 is 100% crossreactive with rat neuromedin U. Immunohistochemical analyses using this antiserum revealed that neuromedin U-immunoreactive structures in rat intestine were confined to the enteric nervous system, implying that neuromedin U may be involved in neuronal regulation of gut function.


Subject(s)
Intestine, Small/metabolism , Neuropeptides/metabolism , Amino Acid Sequence , Animals , Cross Reactions , Ileum/immunology , Ileum/metabolism , Ileum/ultrastructure , Intestine, Small/immunology , Intestine, Small/innervation , Male , Molecular Sequence Data , Neuropeptides/immunology , Rabbits , Radioimmunoassay , Rats , Rats, Inbred Strains
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