ABSTRACT
Canine dirofilariasis is common in Brazil, but molecular diagnosis is rare even though molecular studies increase our knowledge about molecular epidemiology and circulating genotypes from helminths worldwide. This study aims to estimate the prevalence of infection with a modified Knott's test and to perform molecular characterization of Dirofilaria immitis (Leidy, 1856) Railliet and Henry, 1911, in dogs from endemic areas of Maricá and Niterói municipalities, Rio de Janeiro State, Brazil. Molecular characterization was performed in 33 blood samples from dogs positive for microfilariae and 4 adult worms obtained from 2 other dogs. DNA extraction followed by PCR for mitochondrial target 12S rDNA and cytochrome oxidase subunit 1 (COI) of D. immitis were performed, and the amplified products were sequenced. All sequences were identical for both gene targets and showed 100% identity with D. immitis sequences from different animal species from various countries. The study concluded that this genotype of D. immitis might be dispersed worldwide.
Subject(s)
Dirofilaria immitis/genetics , Dirofilariasis/epidemiology , Dog Diseases/epidemiology , Dog Diseases/parasitology , Animals , Brazil/epidemiology , DNA, Helminth/blood , DNA, Helminth/chemistry , DNA, Helminth/isolation & purification , DNA, Ribosomal/chemistry , DNA, Ribosomal/isolation & purification , Dirofilaria immitis/classification , Dirofilariasis/parasitology , Dogs , Electron Transport Complex IV/genetics , Endemic Diseases/veterinary , Genotype , Polymerase Chain Reaction/veterinary , Prevalence , RNA, Ribosomal/geneticsABSTRACT
INTRODUCTION: Abnormalities of liver function tests have been occasionally described in large series of Lyme disease, but only one case of hepatitis directly related to infection have been described in literature. CASE REPORT: A 78-year-old-man, with a past medical history of polymyalgia rheumatica (PMR) who had discontinued corticosteroids two years before, presented a transient acute fever and liver cholestasis and cytolysis after an exposure to tick bites. A few days later, cervical pain occurred and corticosteroids were resumed as a PMR relapse was suspected. Hematogenous dissemination with acute meningoradiculitis and multiple erythema migrans led to conclude to a stage 2 Lyme disease. CONCLUSION: Although hepatitis complicating the course of Lyme disease has been described in literature, the marked inflammation in our patient led us to investigate the possibility of a co-infection. Also, we discuss the responsibility of corticosteroids in clinical worsening of Lyme disease if they are prescribed without concomitant antibiotics.
Subject(s)
Adrenal Cortex Hormones/adverse effects , Hepatitis/etiology , Lyme Disease/complications , Aged , Disease Progression , Hepatitis/diagnosis , Humans , Male , Methotrexate/adverse effects , Prednisone/adverse effectsABSTRACT
We report the case of 74 years-old female patient hospitalized for a ST+ acute coronary syndrome with normal coronary angiography. The association of a patent foramen ovale, a deep venous thrombosis and a pulmonary embolism led us to conclude the diagnosis of paradoxical coronary embolism. This case allows us to remind different etiologies to be considered in case of myocardial infarction with normal coronary arteries, and the interest of transesophageal echocardiography for the diagnosis of its etiology.
Subject(s)
Coronary Vessels/diagnostic imaging , Echocardiography, Transesophageal , Myocardial Infarction/diagnostic imaging , Aged , Coronary Angiography , Female , Humans , Myocardial Infarction/complications , Pulmonary Embolism/complications , Pulmonary Embolism/diagnostic imagingABSTRACT
Foram analisadas 252 amostras de fezes de cães e gatos provenientes dos municípios do Rio de Janeiro e Niterói no período de 1999 a 2000. Foram 212 (84,12
Subject(s)
Dogs/parasitology , Cats , Feces , ParasitesABSTRACT
Foram analisadas 252 amostras de fezes de cães e gatos provenientes dos municípios do Rio de Janeiro e Niterói no períodode 1999 a 2000. Foram 212 (84,12%) amostras de cães e 40 (15,87%) de gatos. Do total de amostras examinadas 81(32,14%), foram positivas para pelo menos uma espécie de parasita. Dos 212 exames de material fecal canino, 70 (33,01%)estavam positivos e de felinos 11 (27,5%). As infecções mistas foram observadas em 10 amostras caninas (14,28%) e quatrofelinas (36,36%). Nas amostras caninas, ovos de ancilostomídeos foram os mais encontrados em 43 (61,43%) amostras,enquanto os coccídeos foram mais freqüentemente observados nas fezes de felinos em três (27,27%) amostras. Quanto àidade dos animais, 37 (52,85%) amostras positivas eram provenientes de animais com mais de 1 ano de idade, indicandoprovável vermifugação dos filhotes, enquanto nos gatos a faixa etária mais parasitada foi de menos de seis meses, com 7(63,63%) positivos.
ABSTRACT
Interventional procedures associated with acute coronary syndromes or performed on saphenous bypass grafts frequently lead to embolic complications, resulting in no-reflow phenomenon, side-branch occlusion, or peri-procedural infarction. The RESCUE thrombo-aspiration system was used in 19 percutaneous coronary interventions. After initial use of the aspiration device, 81% of procedures were followed by stent deployment. TIMI flow 2 or higher was present in 42% at the beginning of the procedure and in 95% at the end. In-hospital MACE rate was 4.76%. This relatively user-friendly technique appears rapid and efficacious in the case of visible intracoronary thrombus.
Subject(s)
Angina Pectoris/complications , Catheterization , Coronary Thrombosis/complications , Coronary Thrombosis/therapy , Myocardial Infarction/complications , Acute Disease , Female , Humans , Male , Middle Aged , Suction , SyndromeABSTRACT
The identification of coronary thrombosis in humans has important prognostic and therapeutic implications. Its recognition calls for invasive techniques: angioscopy, coronarography, and endocoronary ultrasound. Angioscopy allows visualisation of the arterial surface and the detection of thrombus with great sensitivity. Its presence is exclusive to the acute coronary syndromes: 67% in unstable angina, and 75% in myocardial infarction, although it is only present in 27% of cases of stable angina. The relative complexity of its use has led to the abandonment of this technique. Coronarography allows an indirect approach to coronary thrombosis. Certain aspects are evocative such as: intraluminal filling defect, complete occlusion with upstream convexity, ulceration and eccentric type 2 Ambrose classification plaques. As a function of the clinical presentation, the coronarographic views allow a good specificity for the diagnosis of thrombus. The sensitivity is weak, however, compared to angioscopy. Endocoronary ultrasound does not allow identification of fresh thrombus which is not echogenic and does not allow differentiation between older thrombus and lipid plaque. In the future, magnetic resonance imaging could prove interesting in the detection of recent thrombus.
