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1.
PLoS Biol ; 21(11): e3002347, 2023 Nov.
Article in English | MEDLINE | ID: mdl-37930945

ABSTRACT

Many viruses have evolved ways to restructure their host cell's nucleus profoundly and unexpectedly upon infection. In particular, DNA viruses that need to commandeer their host's cellular synthetic functions to produce their progeny can induce the condensation and margination of host chromatin during productive infection, a phenomenon known as virus-induced reorganization of cellular chromatin (ROCC). These ROCC-inducing DNA viruses belong to 5 families (herpesviruses, baculoviruses, adenoviruses, parvoviruses, and geminiviruses) that infect a wide range of hosts and are important for human and ecosystem health, as well as for biotechnology. Although the study of virus-induced ROCC is in its infancy, investigations are already raising important questions, such as why only some DNA viruses that replicate their genomes in the nucleus elicit ROCC. Studying the shared and distinct properties of ROCC-inducing viruses will provide valuable insights into viral reorganization of host chromatin that could have implications for future therapies that target the viral life cycle.


Subject(s)
Ecosystem , Viruses , Humans , DNA Viruses , Chromatin
2.
mBio ; 14(1): e0268622, 2023 02 28.
Article in English | MEDLINE | ID: mdl-36625581

ABSTRACT

We have discovered how Epstein-Barr virus (EBV) induces the reorganization of cellular chromatin (ROCC), in which host chromatin is compacted and marginated within the nucleus, with viral DNA replication occurring in the chromatin-free regions. Five families of DNA viruses induce ROCC: herpesviruses, adenoviruses, parvoviruses, baculoviruses, and geminiviruses. These families infect a variety of hosts, including vertebrates, insects, and plants. They also share several characteristics: they replicate and encapsidate their genomes in the host nucleus and package their genomes unbound by histones. We have identified the viral genes and processes required for EBV's ROCC. Each of EBV's seven core DNA synthesis genes and its origin of lytic replication (oriLyt), in trans, are required, while its protein kinase, BGLF4, and its true late genes are not. Following these findings, we tested the role of EBV lytic DNA amplification in driving ROCC. Surprisingly, the inhibition of EBV's lytic DNA synthesis still supports chromatin compaction but blocks its margination. We propose a two-step model for ROCC. First, the initiation of viral lytic DNA synthesis induces a cellular response that results in global chromatin compaction. Second, the histone-free, productive viral DNA synthesis leads to the margination of compacted chromatin to the nuclear periphery. We have tested this model by asking if the histone-associated simian virus 40 (SV40) DNA synthesis could substitute for oriLyt-mediated synthesis and found that EBV's ROCC is incompatible with SV40 DNA replication. Elucidating EBV's induction of ROCC both illuminates how other viruses can do so and indicates how this spatial control of cellular chromatin benefits them. IMPORTANCE Five families of viruses support the reorganization of cellular chromatin (ROCC), the compaction and margination of host chromatin, upon their productive infection. That they all share this phenotype implies the importance of ROCC in viral life cycles. With Epstein-Barr virus (EBV), a herpesvirus, we show that the viral replication complex and origin of lytic replication (oriLyt) are essential for ROCC. In contrast, its protein kinase and true late genes are not. We show that, unexpectedly, the viral lytic amplification is not required for chromatin compaction but is required for its margination. We propose a two-step model for ROCC: first, global chromatin compaction occurs as a cellular response to the initiation of viral DNA synthesis; then, the accumulation of newly synthesized, histone-free viral DNA leads to cellular chromatin margination. Taken together, our findings provide insights into a process contributing to the productive phase of five families of viruses.


Subject(s)
Epstein-Barr Virus Infections , Herpesvirus 4, Human , Humans , Herpesvirus 4, Human/physiology , Chromatin/metabolism , Virus Replication/genetics , DNA Replication , DNA, Viral/genetics , DNA, Viral/metabolism , Histones/metabolism , Protein Kinases/genetics
3.
ACS Synth Biol ; 11(7): 2259-2266, 2022 07 15.
Article in English | MEDLINE | ID: mdl-35767740

ABSTRACT

The advent of genome-wide clustered regularly interspaced short palindromic repeats (CRISPR) screening has advanced the understanding of molecular systems within cells. Here, we demonstrate the utility of sequentially performed CRISPR knockout screens that use an existing library to explore a biological question across the human genome, and then the remaining cells are used to examine each gene candidate against one common gene of interest. We call this approach "Many vs One" CRISPR screening, made possible by a modified 7SK promoter in place of the U6 promoter to drive expression of a single guide RNA. Inserting this novel 7SK promoter into the ubiquitously used lentiCRISPRv2 backbone is crucial, because it overcomes the need for a substantial increase in CRISPR library coverage during screening, sample processing, and next generation sequencing. This new 7SK vector equals the original lentiCRISPRv2 in lentiviral titer, knockout efficiency, and ease of use.


Subject(s)
CRISPR-Cas Systems , RNA, Guide, Kinetoplastida , CRISPR-Cas Systems/genetics , Gene Editing , Gene Library , Genome , High-Throughput Nucleotide Sequencing , Humans , RNA, Guide, Kinetoplastida/genetics
4.
mBio ; 12(6): e0224321, 2021 12 21.
Article in English | MEDLINE | ID: mdl-34781735

ABSTRACT

The innate immune system serves as frontline defense against pathogens, such as bacteria and viruses. Natural killer (NK) cells are a part of innate immunity and can both secrete cytokines and directly target cells for lysis. NK cells express several cell surface receptors, including NKG2D, which bind multiple ligands. People with deficiencies in NK cells are often susceptible to uncontrolled infection by herpesviruses, such as Epstein-Barr virus (EBV). Infection with EBV stimulates both innate and adaptive immunity, yet the virus establishes lifelong latent infection in memory B cells. We show that the EBV oncogene EBNA1, previously known to be necessary for maintaining EBV genomes in latently infected cells, also plays an important role in suppressing NK cell responses and cell death in newly infected cells. EBNA1 does so by downregulating the NKG2D ligands ULBP1 and ULBP5 and modulating expression of c-Myc. B cells infected with a derivative of EBV that lacks EBNA1 are more susceptible to NK cell-mediated killing and show increased levels of apoptosis. Thus, EBNA1 performs a previously unappreciated role in reducing immune response and programmed cell death after EBV infection, helping infected cells avoid immune surveillance and apoptosis and thus persist for the lifetime of the host. IMPORTANCE Epstein-Barr virus (EBV) is a ubiquitous human pathogen, infecting up to 95% of the world's adult population. Initial infection with EBV can cause infectious mononucleosis. EBV is also linked to several human malignancies, including lymphomas and carcinomas. Although infection by EBV alerts the immune system and causes an immune response, the virus persists for life in memory B cells. We show that the EBV protein EBNA1 can downregulate several components of the innate immune system linked to natural killer (NK) cells. This downregulation of NK cell activity translates to lower killing of EBV-infected cells and is likely one way that EBV escapes immune surveillance after infection. Additionally, we show that EBNA1 reduces apoptosis in newly infected B cells, allowing more of these cells to survive. Taken together, our findings uncover new functions of EBNA1 and provide insights into viral strategies to survive the initial immune response postinfection.


Subject(s)
Apoptosis , Epstein-Barr Virus Infections/immunology , Epstein-Barr Virus Nuclear Antigens/immunology , Herpesvirus 4, Human/physiology , Killer Cells, Natural/immunology , Memory B Cells/virology , Cell Line , Epstein-Barr Virus Infections/physiopathology , Epstein-Barr Virus Infections/virology , Epstein-Barr Virus Nuclear Antigens/genetics , GPI-Linked Proteins/genetics , GPI-Linked Proteins/immunology , Herpesvirus 4, Human/genetics , Herpesvirus 4, Human/immunology , Host-Pathogen Interactions , Humans , Intracellular Signaling Peptides and Proteins/genetics , Intracellular Signaling Peptides and Proteins/immunology , Killer Cells, Natural/cytology , Memory B Cells/cytology , Memory B Cells/immunology
5.
Front Microbiol ; 12: 737975, 2021.
Article in English | MEDLINE | ID: mdl-34484173
6.
Viruses ; 13(8)2021 07 28.
Article in English | MEDLINE | ID: mdl-34452344

ABSTRACT

Epstein-Barr virus (EBV) and Kaposi's sarcoma-associated herpesvirus (KSHV) independently cause human cancers, and both are maintained as plasmids in tumor cells. They differ, however, in their mechanisms of segregation; EBV partitions its genomes quasi-faithfully, while KSHV often clusters its genomes and partitions them randomly. Both viruses can infect the same B-cell to transform it in vitro and to cause primary effusion lymphomas (PELs) in vivo. We have developed simulations based on our measurements of these replicons in B-cells transformed in vitro to elucidate the synthesis and partitioning of these two viral genomes when in the same cell. These simulations successfully capture the biology of EBV and KSHV in PELs. They have revealed that EBV and KSHV replicate and partition independently, that they both contribute selective advantages to their host cell, and that KSHV pays a penalty to cluster its genomes.


Subject(s)
B-Lymphocytes/virology , Cell Transformation, Viral , Coinfection/virology , Epstein-Barr Virus Infections/virology , Herpesviridae Infections/virology , Herpesvirus 4, Human/physiology , Herpesvirus 8, Human/physiology , Herpesvirus 4, Human/genetics , Herpesvirus 8, Human/genetics , Humans , Lymphoma, Primary Effusion/virology , Virus Replication
7.
Front Microbiol ; 12: 643327, 2021.
Article in English | MEDLINE | ID: mdl-33664726

ABSTRACT

Epstein-Barr virus (EBV) encodes more than 40 miRNAs that target cellular mRNAs to aid its infection, replication, and maintenance in individual cells and in its human host. Importin-7 (IPO7), also termed Imp7 or RanBPM7, is a nucleocytoplasmic transport protein that has been frequently identified as a target for two of these viral miRNAs. How the viral life cycle might benefit from regulating IPO7 has been unclear, though. We demonstrate with CRISPR-Cas9 mutagenesis that IPO7 is essential in at least three cells lines and that increasing its levels of expression inhibits growth of infected cells. EBV thus regulates the level of IPO7 to limit its accumulation consistent with its being required for survival of its host cell.

8.
Microorganisms ; 8(11)2020 Nov 19.
Article in English | MEDLINE | ID: mdl-33228078

ABSTRACT

Epstein-Barr Virus (EBV) contributes to the development of lymphoid and epithelial malignancies. While EBV's latent phase is more commonly associated with EBV-associated malignancies, there is increasing evidence that EBV's lytic phase plays a role in EBV-mediated oncogenesis. The lytic phase contributes to oncogenesis primarily in two ways: (1) the production of infectious particles to infect more cells, and (2) the regulation of cellular oncogenic pathways, both cell autonomously and non-cell autonomously. The production of infectious particles requires the completion of the lytic phase. However, the regulation of cellular oncogenic pathways can be mediated by an incomplete (abortive) lytic phase, in which early lytic gene products contribute substantially, whereas late lytic products are largely dispensable. In this review, we discuss the evidence of EBV's lytic phase contributing to oncogenesis and the role it plays in tumor formation and progression, as well as summarize known mechanisms by which EBV lytic products regulate oncogenic pathways. Understanding the contribution of EBV's lytic phase to oncogenesis will help design ways to target it to treat EBV-associated malignancies.

9.
Front Cell Infect Microbiol ; 10: 606412, 2020.
Article in English | MEDLINE | ID: mdl-33505922

ABSTRACT

Epstein-Barr Virus (EBV) can transform B cells and contributes to the development of Burkitt lymphoma and other cancers. Through decades of study, we now recognize that many of the viral genes required to transform cells are not expressed in EBV-positive Burkitt lymphoma (BL) tumors, likely due to the immune pressure exerted on infected cells. This recognition has led to the hypothesis that the loss of expression of these viral genes must be compensated through some mechanisms. Recent progress in genome-wide mutational analysis of tumors provides a wealth of data about the cellular mutations found in EBV-positive BLs. Here, we review common cellular mutations found in these tumors and consider how they may compensate for the viral genes that are no longer expressed. Understanding these mutations and how they may substitute for EBV's genes and contribute to lymphomagenesis can serve as a launchpad for more mechanistic studies, which will help us navigate the sea of genomic data available today, and direct the discoveries necessary to improve the treatment of EBV-positive BLs.


Subject(s)
Burkitt Lymphoma , Epstein-Barr Virus Infections , B-Lymphocytes , Burkitt Lymphoma/genetics , Herpesvirus 4, Human/genetics , Humans
10.
Proc Natl Acad Sci U S A ; 116(49): 24630-24638, 2019 12 03.
Article in English | MEDLINE | ID: mdl-31744871

ABSTRACT

Herpesviruses must amplify their DNA to load viral particles and they do so in replication compartments. The development and functions of replication compartments during DNA amplification are poorly understood, though. Here we examine 2 functionally distinct replicons in the same cells to dissect DNA amplification within replication compartments. Using a combination of single-cell assays, computational modeling, and population approaches, we show that compartments initially were seeded by single genomes of Epstein-Barr virus (EBV). Their amplification subsequently took 13 to 14 h in individual cells during which their compartments occupied up to 30% of the nucleus and the nuclear volume grew by 50%. The compartmental volumes increased in proportion to the amount of DNA and viral replication proteins they contained. Each compartment synthesized similar levels of DNA, indicating that the total number of compartments determined the total levels of DNA amplification. Further, the amplification, which depended on the number of origins, was regulated differently early and late during the lytic phase; early during the lytic phase, the templates limited DNA synthesis, while later the templates were in excess, coinciding with a decline in levels of the viral replication protein, BMRF1, in the replication compartments. These findings show that replication compartments are factories in which EBV DNA amplification is both clonal and coordinated.


Subject(s)
DNA Replication/genetics , DNA, Viral/biosynthesis , Herpesvirus 4, Human/physiology , Replicon/genetics , Virus Replication/genetics , Antigens, Viral/genetics , Antigens, Viral/metabolism , Cell Nucleus/genetics , Cell Nucleus/metabolism , HEK293 Cells , Humans , Intravital Microscopy
11.
Proc Natl Acad Sci U S A ; 116(33): 16519-16528, 2019 08 13.
Article in English | MEDLINE | ID: mdl-31363046

ABSTRACT

Primary effusion lymphomas (PELs) are causally associated with Kaposi's sarcoma-associated herpesvirus (KSHV) and 86% of PELs are coinfected with Epstein-Barr virus (EBV). Understanding how PELs develop has been impaired by the difficulty of infecting B cells with KSHV in vitro, and the inability of KSHV to transform them. We show that EBV supports an optimal coinfection of 2.5% of peripheral B cells by KSHV. This coinfection requires 1 or more transforming genes of EBV but not entry into KSHV's lytic cycle. We demonstrate that dually infected B cells are stably transformed in vitro and show that while both viruses can be maintained, different cells exhibit distinct, transformed properties. Transformed cells that grow to predominate in a culture express increased levels of most KSHV genes and differentially express a subset of cellular genes, as do bona fide PEL cells. These dually infected peripheral B cells are thus both stably transformed and allow in vitro molecular dissection of early steps in the progression to lymphomagenesis.


Subject(s)
B-Lymphocytes/virology , Carcinogenesis/immunology , Cell Transformation, Neoplastic/pathology , Herpesvirus 8, Human/physiology , Lymphoma/pathology , Lymphoma/virology , Sarcoma, Kaposi/immunology , Sarcoma, Kaposi/virology , CD40 Ligand/metabolism , Cell Proliferation , Gene Expression Regulation, Viral , Green Fluorescent Proteins/metabolism , Herpesvirus 4, Human/physiology , Herpesvirus 8, Human/genetics , Humans , Interleukin-4/metabolism , Lymphocyte Activation/immunology , Sarcoma, Kaposi/pathology
12.
J Virol ; 93(8)2019 04 15.
Article in English | MEDLINE | ID: mdl-30700606

ABSTRACT

The Epstein-Barr virus (EBV) lytic phase, like those of all herpesviruses, proceeds via an orderly cascade that integrates DNA replication and gene expression. EBV early genes are expressed independently of viral DNA amplification, and several early gene products facilitate DNA amplification. On the other hand, EBV late genes are defined by their dependence on viral DNA replication for expression. Recently, a set of orthologous genes found in beta- and gammaherpesviruses have been determined to encode a viral preinitiation complex (vPIC) that mediates late gene expression. The EBV vPIC requires an origin of lytic replication in cis, implying that the vPIC mediates transcription from newly replicated DNA. In agreement with this implication, EBV late gene mRNAs localize to replication factories. Notably, these factories exclude canonical histones. In this review, we compare and contrast the mechanisms and epigenetics of EBV early and late gene expression. We summarize recent findings, propose a model explaining the dependence of EBV late gene expression on lytic DNA amplification, and suggest some directions for future study.


Subject(s)
Epigenesis, Genetic/physiology , Gene Expression Regulation, Viral/physiology , Herpesvirus 4, Human/physiology , Histones/metabolism , Transcription, Genetic/physiology , Virus Replication/physiology , DNA Replication/physiology , DNA, Viral/biosynthesis , Humans
13.
PLoS Biol ; 16(11): e3000062, 2018 11.
Article in English | MEDLINE | ID: mdl-30481166

ABSTRACT

High-risk human papillomaviruses (HPVs) are a major cause of cancers. HPVs infect epithelial cells, and viral oncogenes disrupt several cellular processes, including cell division, differentiation, and apoptosis. Expression of these oncogenes is relatively low in undifferentiated epithelial cells but increases in differentiating cells by unknown mechanisms. In a new study, Parish and colleagues unveil how two cellular proteins, CCCTC-binding factor (CTCF) and Yin Yang 1 (YY1), mediate looping of the HPV18 genome, which regulates expression of viral oncogenes in both dividing and differentiating epithelial cells.


Subject(s)
Oncogene Proteins, Viral , Papillomaviridae , CCCTC-Binding Factor , Cell Differentiation , Human papillomavirus 18 , Humans
15.
J Virol ; 92(9)2018 05 01.
Article in English | MEDLINE | ID: mdl-29467315

ABSTRACT

The human tumor viruses that replicate as plasmids (we use the term plasmid to avoid any confusion in the term episome, which was coined to mean DNA elements that occur both extrachromosomally and as integrated forms during their life cycles, as does phage lambda) share many features in their DNA synthesis. We know less about their mechanisms of maintenance in proliferating cells, but these mechanisms must underlie their partitioning to daughter cells. One amazing implication of how these viruses are thought to maintain themselves is that while host chromosomes commit themselves to partitioning in mitosis, these tumor viruses would commit themselves to partitioning before mitosis and probably in S phase shortly after their synthesis.


Subject(s)
DNA Replication/genetics , DNA, Viral/genetics , Herpesvirus 4, Human/genetics , Herpesvirus 8, Human/genetics , Papillomaviridae/genetics , Plasmids/genetics , DNA, Viral/biosynthesis , Humans , Mitosis/genetics , Replicon/genetics
16.
J Cell Biol ; 216(9): 2745-2758, 2017 09 04.
Article in English | MEDLINE | ID: mdl-28696226

ABSTRACT

Genetic elements that replicate extrachromosomally are rare in mammals; however, several human tumor viruses, including the papillomaviruses and the gammaherpesviruses, maintain their plasmid genomes by tethering them to cellular chromosomes. We have uncovered an unprecedented mechanism of viral replication: Kaposi's sarcoma-associated herpesvirus (KSHV) stably clusters its genomes across generations to maintain itself extrachromosomally. To identify and characterize this mechanism, we developed two complementary, independent approaches: live-cell imaging and a predictive computational model. The clustering of KSHV requires the viral protein, LANA1, to bind viral genomes to nucleosomes arrayed on both cellular and viral DNA. Clustering affects both viral partitioning and viral genome numbers of KSHV. The clustering of KSHV plasmids provides it with an effective evolutionary strategy to rapidly increase copy numbers of genomes per cell at the expense of the total numbers of cells infected.


Subject(s)
Chromosomes , DNA Replication , DNA, Viral/genetics , Genome, Viral , Genomic Instability , Herpesvirus 8, Human/genetics , Virus Replication , Antigens, Viral/genetics , Antigens, Viral/metabolism , Computer Simulation , DNA, Viral/biosynthesis , Evolution, Molecular , Gene Expression Regulation, Viral , HEK293 Cells , HeLa Cells , Herpesvirus 4, Human/genetics , Herpesvirus 8, Human/growth & development , Herpesvirus 8, Human/metabolism , Host-Pathogen Interactions , Humans , In Situ Hybridization, Fluorescence , Microscopy, Confocal , Microscopy, Video , Models, Genetic , Nuclear Proteins/genetics , Nuclear Proteins/metabolism , Protein Binding , Time Factors , Time-Lapse Imaging , Transfection
17.
Virology ; 505: 148-154, 2017 05.
Article in English | MEDLINE | ID: mdl-28259048

ABSTRACT

Epstein-Barr virus (EBV) encodes multiple miRNAs known to contribute to its pathogenicity. Previous studies have found that the levels of some EBV miRNAs are 10-100 fold higher in biopsies and in tumor xenografts than in cells grown in culture. We have asked if these increased levels reflect transcriptional enhancement resulting from the tumor microenvironment, selection for increased levels of the EBV genome, or both. We measured the levels of BART miRNAs and their DNA templates in tumor xenografts induced from EBV-positive gastric carcinoma cells and EBV-negative gastric carcinoma cells expressing plasmid replicons encoding these miRNAs. We focused on BART miRNAs which are expressed in all tumors and found that they provide tumors selective growth advantages as xenografts. Stem-loop PCR and real-time PCR revealed that the xenografts expressed both higher levels of some miRNAs and viral DNA templates than did the corresponding cells in culture.


Subject(s)
Carcinoma/genetics , Epstein-Barr Virus Infections/genetics , Herpesvirus 4, Human/genetics , MicroRNAs/genetics , Stomach Neoplasms/genetics , Animals , Cell Line, Tumor , DNA, Viral/genetics , Epstein-Barr Virus Infections/virology , Gene Expression Regulation, Viral , Genome, Viral/genetics , Herpesvirus 4, Human/metabolism , Humans , Mice , Neoplasm Transplantation , Transcription, Genetic/genetics , Transplantation, Heterologous , Tumor Microenvironment
18.
Proc Natl Acad Sci U S A ; 113(42): E6467-E6475, 2016 10 18.
Article in English | MEDLINE | ID: mdl-27698133

ABSTRACT

Infection with Epstein-Barr virus (EBV) affects most humans worldwide and persists life-long in the presence of robust virus-specific T-cell responses. In both immunocompromised and some immunocompetent people, EBV causes several cancers and lymphoproliferative diseases. EBV transforms B cells in vitro and encodes at least 44 microRNAs (miRNAs), most of which are expressed in EBV-transformed B cells, but their functions are largely unknown. Recently, we showed that EBV miRNAs inhibit CD4+ T-cell responses to infected B cells by targeting IL-12, MHC class II, and lysosomal proteases. Here we investigated whether EBV miRNAs also counteract surveillance by CD8+ T cells. We have found that EBV miRNAs strongly inhibit recognition and killing of infected B cells by EBV-specific CD8+ T cells through multiple mechanisms. EBV miRNAs directly target the peptide transporter subunit TAP2 and reduce levels of the TAP1 subunit, MHC class I molecules, and EBNA1, a protein expressed in most forms of EBV latency and a target of EBV-specific CD8+ T cells. Moreover, miRNA-mediated down-regulation of the cytokine IL-12 decreases the recognition of infected cells by EBV-specific CD8+ T cells. Thus, EBV miRNAs use multiple, distinct pathways, allowing the virus to evade surveillance not only by CD4+ but also by antiviral CD8+ T cells.


Subject(s)
CD8-Positive T-Lymphocytes/immunology , Epstein-Barr Virus Infections/immunology , Epstein-Barr Virus Infections/virology , Herpesvirus 4, Human/genetics , Herpesvirus 4, Human/immunology , Immunologic Surveillance/genetics , MicroRNAs/genetics , RNA, Viral/genetics , Antigen Presentation , B-Lymphocytes/immunology , B-Lymphocytes/metabolism , B-Lymphocytes/virology , CD8-Positive T-Lymphocytes/metabolism , Cell Line , Cell Survival/immunology , Cytokines/metabolism , Cytotoxicity, Immunologic , Epitopes, T-Lymphocyte/metabolism , Epstein-Barr Virus Infections/metabolism , Gene Expression Regulation, Viral , Histocompatibility Antigens Class I/immunology , Histocompatibility Antigens Class I/metabolism , Humans , Immune Evasion , Receptors, Cytokine/metabolism
19.
J Exp Med ; 213(10): 2065-80, 2016 09 19.
Article in English | MEDLINE | ID: mdl-27621419

ABSTRACT

Epstein-Barr virus (EBV) is a tumor virus that establishes lifelong infection in most of humanity, despite eliciting strong and stable virus-specific immune responses. EBV encodes at least 44 miRNAs, most of them with unknown function. Here, we show that multiple EBV miRNAs modulate immune recognition of recently infected primary B cells, EBV's natural target cells. EBV miRNAs collectively and specifically suppress release of proinflammatory cytokines such as IL-12, repress differentiation of naive CD4(+) T cells to Th1 cells, interfere with peptide processing and presentation on HLA class II, and thus reduce activation of cytotoxic EBV-specific CD4(+) effector T cells and killing of infected B cells. Our findings identify a previously unknown viral strategy of immune evasion. By rapidly expressing multiple miRNAs, which are themselves nonimmunogenic, EBV counteracts recognition by CD4(+) T cells and establishes a program of reduced immunogenicity in recently infected B cells, allowing the virus to express viral proteins required for establishment of life-long infection.


Subject(s)
CD4-Positive T-Lymphocytes/immunology , Herpesvirus 4, Human/genetics , Interleukin-12/metabolism , MicroRNAs/genetics , Peptides/metabolism , Antigen Presentation , B-Lymphocytes/immunology , B-Lymphocytes/virology , Cell Death , Cell Differentiation , Cell Membrane/metabolism , Cytokines/metabolism , HEK293 Cells , Humans , Immunity , Inflammation Mediators/metabolism , Lysosomes/metabolism , MicroRNAs/metabolism , Receptors, Cell Surface/metabolism , Species Specificity , Th1 Cells/cytology , Th1 Cells/immunology
20.
Annu Rev Virol ; 3(1): 359-372, 2016 09 29.
Article in English | MEDLINE | ID: mdl-27578440

ABSTRACT

The intrinsic properties of different viruses have driven their study. For example, the capacity for efficient productive infection of cultured cells by herpes simplex virus 1 has made it a paradigm for this mode of infection for herpesviruses in general. Epstein-Barr virus, another herpesvirus, has two properties that have driven its study: It causes human cancers, and it exhibits a tractable transition from its latent to its productive cycle in cell culture. Here, we review our understanding of the path Epstein-Barr virus follows to move from a latent infection to and through its productive cycle. We use information from human infections to provide a framework for describing studies in cell culture and, where possible, the molecular resolutions from these studies. We also pose questions whose answers we think are pivotal to understanding this path, and we provide answers where we can.


Subject(s)
Herpesvirus 4, Human/growth & development , Virus Activation/genetics , Virus Latency/genetics , Virus Replication/genetics , Cells, Cultured , DNA, Viral/metabolism , Epstein-Barr Virus Infections/genetics , Epstein-Barr Virus Infections/virology , Epstein-Barr Virus Nuclear Antigens/metabolism , Gene Expression Regulation, Viral/genetics , Herpesvirus 4, Human/genetics , Humans , Virus Assembly/genetics
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