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2.
Article in Russian | MEDLINE | ID: mdl-6776722

ABSTRACT

Using the method proposed by Gillies and Govan and their indicator strains, 342 P. aeruginosa strains isolated from the patients were studied in respect to their pyocinogenicity and typed according to the production of different types of pyocins. Besides, in 206 cultures the pyocin sensitivity of 16 standard P. aeruginosa strains (5 strains obtained from Govan and 11 strains provided by the authors) was determined. All the tested cultures fell into 23 pyocin types; of these, types I and X occured most frequently, 56 strains identified by means of indicators could not be typed due to the fact that the corresponding pyocin types were absent in Govan's scheme. The cultures isolated from the patients and the environmental objects during the outbreak of P. aeruginosa in a hospital were proved to belong to the same pyocin type (III). The double typing of the cultures, according to pyocin production and pyocin sensitivity, allowed to determine individual characteristics of 75% of the tested cultures.


Subject(s)
Bacteriocins/biosynthesis , Pseudomonas aeruginosa/classification , Pyocins/biosynthesis , Humans , Methods , Pseudomonas aeruginosa/drug effects , Pseudomonas aeruginosa/metabolism , Pyocins/pharmacology
14.
Zh Mikrobiol Epidemiol Immunobiol ; (8): 122-6, 1975 Aug.
Article in Russian | MEDLINE | ID: mdl-1106079

ABSTRACT

The authors consider the current state of the problem and personal data on the role of L-forms of bacteria and mycoplasma in human infectious pathology. L-forms of bacteria were isolated from the urine in 10% of the patients suffering from pyelonephritis and mycoplasmae--in over one third of all the patients both with pyelonephritis and with cystites and prostatitis. Some of the isolated strains of mycoplasmae produced a cytopathogenic effect on the cell cultures, whereas the rest caused a latent infection. Drug sensitivity determined to ten antibacterial preparations with a wide range of action differed in strains of the same species. The efficacy of treatment and the prospects of microbiological studies in cases with atypical and latent courses of inflammatory processes of the urogenital organs in discussed.


Subject(s)
L Forms/pathogenicity , Mycoplasma Infections/microbiology , Mycoplasma/pathogenicity , Urologic Diseases/microbiology , Bacteriuria/microbiology , Cell Membrane/physiology , Cell Wall/ultrastructure , Chronic Disease , Drug Resistance, Microbial , Humans , Kidney Diseases/microbiology , L Forms/cytology , L Forms/metabolism , Mycoplasma/cytology , Mycoplasmataceae/cytology , Protoplasts/physiology , Urinary Tract Infections/microbiology , Urine/microbiology , Urogenital System/microbiology
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