[Changes in the levels of erythrocyte membrane proteins in hypertension]. / Izmenenie kolichestvennogo sootnosheniia membrannykh belkov éritrotsitov pri gipertonicheskoi bolezni.

The distribution of specific proteins in erythrocyte membranes was studied in patients with essential hypertension (EH) (n = 44), secondary hypertension of renal genesis (n = 42), and healthy persons (n = 44). Densitograms of gel analyzed after electrophoresis of erythrocyte ghosts showed a twofold increase in polypeptide levels in EH patients as compared to those in health persons: the band being 4.5 (Mw = 52-59 kD) and 6 (Mw = 35 kD), respectively. Radioimmunoassay has demonstrated that the amount of monoclonal antibodies bound to fragmented erythrocyte membranes from EH patients is greater by at least 28% than that in healthy persons. Patients with secondary arterial hypertension of renal genesis showed no significant difference in monoclonal antibody binding as compared to the controls.

[Detection of concomitant pathology in patients with arterial hypertension by structural and functional changes of erythrocyte membranes]. / Vyiavlenie sochetannoi patologii u bo'lnykh arteria'lnoi gipertenziei po strukturno-funktsiona'lnym izmeneniiam membran éritrotsitov.

The values of the Na-Li+ countertransport rate and the degree of monoclonal antibody binding with the erythrocyte membranes were compared in patients with different forms of arterial hypertension (AH) to identify combined pathology and the precursors of surgery effectiveness. Among the 27 examined AH patients 7 underwent surgery in accordance with the initial clinical diagnosis. The membrane tests helped to detect 3 patients with combined pathology: chronic pyelonephritis or chronic diffuse glomerulonephritis in combination with AH. Thus, the membrane tests were found to supplement each other in the assessment of surgery effectiveness and the perspectives for identification of combined pathology in patients with symptomatic AH of renal genesis.

[Binding of monoclonal antibodies to fragmented erythrocyte membranes in patients with arterial hypertension]. / Sviazyvanie monoklonal'nykh antitel s fragmentirovannymi membranami éritrotsitov bol'nykh s arterial'nymi gipertenziiami.

The data obtained by radioimmunoassay indicate that monoclonal antibodies (MA) to sarcoplasmic reticulum membranes can also bind to erythrocyte membranes. The binding of MA to fragmented erythrocyte membranes from patients with essential hypertension (n = 20) is 35% higher, as compared to normal subjects (n = 14) or patients with secondary (renal) hypertension (n = 9). Possible use of radioimmunoassays for MA is discussed with reference to differential diagnosis of arterial hypertensions.

Distribution of proteins in erythrocyte membranes from patients with hypertension.

The distribution of characteristic proteins in erythrocyte membranes was studied in patients with essential hypertension (EH) (n = 44), secondary hypertension of renal genesis (n = 42), and healthy persons (n = 44). Densitograms of gels analyzed after electrophoresis of erythrocyte ghosts showed a twofold increase in the amount of band 4.5 (Mw = 52-59 kD) and band 6 (Mw = 35 kD) polypeptides in EH patients as compared to that in healthy persons. Radioimmunoassay with monoclonal antibodies obtained to the sarcoplasmic reticulum (SR) of heart muscle has demonstrated that the amount of the antibodies bound to fragmented erythrocyte membranes from EH persons is greater by at least 28% than that in healthy people. Patients with secondary arterial hypertension of renal genesis did not reveal a significant difference in binding of monoclonal antibodies as compared to the control group. Thus, erythrocyte membranes from EH subjects are different from those taken from the blood of healthy people by the increased amount of bands 4.5 and 6 proteins.