Assembly of Alternated Multivalent Ion/Polyelectrolyte Layers on Colloidal Particles. Stability of the Multilayers and Encapsulation of Macromolecules into Polyelectrolyte Capsules.

Alternating adsorption of multivalent ions and oppositely charged polyelectrolytes on colloid particles has been investigated. Multilayer films composed of Tb(3+)/polysterene sulfonate (PSS) and 4-pyrene sulfate/polyallylamine (PAH) were successfully assembled on polysterene sulfonate (PS) and melamine formaldehyde (MF) latex particles. The amount of assembled material was estimated by fluorescence and the linear growth of the film versus the number of layers was demonstrated. These multilayers are not stable and can be decomposed by salt and temperature. Dissolution of MF particles leads to formation of hollow capsules consisting of multivalent ion/polyelectrolyte multilayers. Comparative analysis of the capsules was done by confocal and scanning force microscopy. Complex hollow spheres consisting of Tb(3+)/PSS or 4-PS/PAH as an inner shell and stable PSS/PAH as an outer shell were produced. Due to selective permeability of the outer shell after degradation of the inner shell the multivalent ions are released out of the capsule while the polyelectrolytes fill the capsule interior. This is indicative of swelling of the capsule by osmotic pressure. The filled capsules were studied by confocal and scanning electron microscopy. Possibilities of encapsulating macromolecules in defined amounts per capsule are discussed. Copyright 2000 Academic Press.

Microencapsulation of Organic Solvents in Polyelectrolyte Multilayer Micrometer-Sized Shells.

Hollow-shell micrometer-sized particles were fabricated in aqueous media by stepwise deposition of oppositely charged polyelectrolytes onto melamine latex particles and biological cells with a dissolution of the core afterward. It is demonstrated that these shells can be suspended in various organic media, such as methanol, ethanol, pentanol, hexanol, octanol, octane, and decane, by a gradual solvent exchange. At this stage of the procedure the shells contain the respective organic solvent. Oil suspensions in water are then formed by transferring the particles from the organic media into water, without the use of any further surfactant addition. By an additional adsorption step employing phospholipids, it is possible to obtain a dispersion of shells in organic solvents containing an aqueous solution inside. AFM measurements are provided which show that the shells preserve their integrity in the different solvents. Confocal microscopy is employed to demonstrate encapsulation of solvents and the presence of lipids. Copyright 1999 Academic Press.