ABSTRACT
According to the competitive ELISA test data, new preparations of monoclonal antibodies (MAb) to respiratory syncytial virus (RSV) differed in their blocking activity and they were directed to 3 different virus epitopes. MAb 9C5 and MAb 131-2A (CDC, Atlanta) competed against each other strongly and they were directed to epitope F1a of RSV F-protein. MAbs 8C5 and 10D8 showed a two-way blocking and were presumably topologically linked. MAb 8B10 did not compete against other MAbs. The fact that MAbs 8B10, 9C5, and 10D8 may be used in indirect ELISA test to detect RSV antigens was shown. The specific activity of MAbs 9C5 was observed, by applying low antibody concentrations to ng/mg. The highest specific activity using the homologous pair MAbs-con 9C5 and 8C5 was observed in direct ELISA employing MAbs for capture and peroxidase conjugate of MAbs for detection. MAbs 8B10 were successfully used for direct (FITC-conjugate of MAbs) and indirect immunofluorescence detection of RSV antigens in the infected cell cultures and clinical materials from patients.
Subject(s)
Antibodies, Monoclonal , Enzyme-Linked Immunosorbent Assay , Fluorescent Antibody Technique, Indirect , Respiratory Syncytial Viruses/immunology , Animals , Antibodies, Viral/analysis , Antigens, Viral/analysis , Epitopes , HeLa Cells , Humans , Mice , Respiratory Syncytial Viruses/geneticsABSTRACT
The paper describes the procedure for producing monoclonal antibodies to human respiratory asyncytial virus using hybridoma technology. Two groups of antibodies were identified in the study of their specific activity. The representatives of the first group preferably reacted with purified virus antigens. Those of the second one binded mostly to unpurified virus antigens from the cultural fluid. The antibodies of these groups appear to be different in their epitope specificity.
