ABSTRACT
Biogas production and municipal wastewater COD removal at low temperatures by granulated anaerobic biomass were investigated. Two anaerobic granule reactors were operated continuously for 1025 days by stepwise increase of organic loading from 1.3 to 15.2 g CODdissolved·l-1·d-1 at 25, 16, 12, 8.5, 5.5, and 2.5 °C. The sustained reactor performance was evaluated by COD removal efficiency, methane production, and microbial community analysis. Stable COD removal of 50-70% were achieved at 25-8.5 °C and up to 15 g CODdissolved·l-1·d-1, and no significant temperature effect was observed on specific methane production rate and yield. Below 8.5 °C, COD removal and methane yields reduced, but still significant methane formation was observed even at 2.5 °C. More than 90% of COD removed was converted to methane. Methanogenic archaea communities showed that temperature changes affected the major methane formation pathways, which explains temperature adaptability of the granules.
