ABSTRACT
Proton magnetic resonance Spectroscopy (PMRS) has been used to study the differences between immortalized fibroblasts and fibrosarcoma cells of different grade. One and two dimensional purged correlation spectroscopy (PCOSY) have been used to assess intact viable fibroblast and fibrosarcoma cells, and differences in the triglyceride, cellular metabolite, and cell surface fucosylation patterns between the three cell lines have been observed. The clinical implication of this study is the potential use of PMRS as an adjunct to conventional histopathology.
Subject(s)
Fibrosarcoma/metabolism , Fibrosarcoma/pathology , 3T3 Cells/cytology , 3T3 Cells/metabolism , Amino Acids/metabolism , Animals , Cell Cycle , Cell Line, Transformed , Cell Membrane/metabolism , Cell Survival , Fucose/analysis , Magnetic Resonance Spectroscopy/methods , Mice , Phospholipids/metabolism , Triglycerides/metabolism , Tumor Cells, CulturedABSTRACT
Twelve patients with high grade osteosarcomas of the extremities were treated with two cycles of induction chemotherapy using adriamycin and cis-platinum and sandwich radiation between the two cycles (4000 rads). Ten patients underwent amputation or disarticulation, two patients had wide excision followed by endoprosthesis. The specimen was assessed for grade of necrosis. The Disease Free Survival at a minimum follow-up period of 26 months and median follow-up period of 35.5%. All the five patients who developed distant metastases had shown only a grade I necrosis in the tumour.
