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1.
Contemp Clin Dent ; 8(2): 264-271, 2017.
Article in English | MEDLINE | ID: mdl-28839414

ABSTRACT

BACKGROUND: Flexural strength (FS) of denture base resins (DBRs) had been improved by reinforcing it with different glass fibers. However, a limited data are available on the effect of glass fiber reinforcement with conventional heat-cured resin after prolonged water storage. AIMS AND OBJECTIVES: This study aimed to evaluate the reinforcing effect of novel S-glass and nylon fibers on the FS of acrylic DBRs. It also aimed to evaluate the effect of glass fiber reinforcement on the FS of acrylic DBRs after a prolonged storage in water. MATERIALS AND METHODS: One hundred and sixty identical specimens were fabricated in specially designed molds according to the manufacturer's instructions. The three experimental groups were prepared consisting of conventional (unreinforced) acrylic resin, novel S-glass fiber-reinforced and nylon fiber-reinforced acrylic resin. The specimens were fabricated in a standardized fashion for each experimental group. Each group was further subdivided into two groups on the basis of storage conditions (dry and wet). FS was tested using a three-point universal testing machine at a crosshead speed of 5 mm/min. Glass fiber-reinforced group was further tested after prolonged storage in distilled water. Entered data were statistically analyzed with one-way ANOVA and least significant difference post hoc test. RESULTS: In this study, statistically significant differences were noted in the FS of all the groups. S-glass fiber-reinforced group had highest FS compared to the other two groups (P < 0.001). Nylon fiber-reinforced group had lowest FS. All the groups stored in distilled water revealed a decrease in strength compared to those stored in dry atmosphere. Among wet specimens, those stored for 3 weeks had a significantly higher FS than those stored at one and 2 weeks (P < 0.01). CONCLUSION: Within the limitations of this investigation, the FS of heat-cured acrylic DBR was improved after reinforcement with glass fibers. It can be recommended to strengthen distal extension partial and complete denture bases. Nylon fibers may not be desirable for strengthening acrylic denture bases.

2.
J Indian Prosthodont Soc ; 16(1): 30-5, 2016.
Article in English | MEDLINE | ID: mdl-27134425

ABSTRACT

CONTEXT: Researchers have concentrated their focus on denture wearer's attitude and practice toward denture cleansing despite the fact that they should be more focused on the attitudes of the dentists' themselves towards patient education at the time of denture delivery. It is an obligation of every dentist to motivate, instruct and provide the means and methods of plaque control for their patients. AIMS: The aim was to assess the denture hygiene knowledge, attitudes and practice towards patient education in denture care among dental practitioners (DPs) of Jabalpur city, Madhya Pradesh, India. MATERIAL AND METHODS: A total of 168 dental practitioners completed a comprehensive questionnaire. All participants signed an informed consent before answering the questionnaire. The institutional review committee approved the study. STATISTICAL ANALYSIS: Chi-square test for non-parametric study was employed to determine the statistical difference between the two groups. A P-value of 0.05 was considered to be statistically significant. RESULTS: Most of the subjects were qualified with a bachelor degree 142 (85%). 25 (18%) subjects did not associate oral biofilms on complete denture with conditions like denture stomatitis and other serious systemic diseases. Approximately half of the DPs 69 (48%) and specialists 8 (31%) agreed that explaining denture hygiene instructions to old patients can be very time consuming. A recall program for their patients is of importance according to 39 (27%) of DPs and 3 (12%) specialists. CONCLUSIONS: It may be concluded that the study subjects had limited knowledge of denture cleansing materials and denture hygiene importance. Attitudes varied among the subjects when it came to sharing information with their patients.

3.
Ann Med Health Sci Res ; 6(6): 348-355, 2016.
Article in English | MEDLINE | ID: mdl-28540102

ABSTRACT

BACKGROUND: Dental undergraduate students work on extracted human teeth in preclinical practical's to learn technical skills before entering the clinics and delivering dental care to the patients. AIM: The aim of the present investigation was to assess the awareness and knowledge toward sterilization/disinfection methods of extracted human teeth in a selected group of Indian dental students. MATERIALS AND METHODS: In this descriptive cross-sectional study, the participants consisted of 2nd-, 3rd-, 4th-, and 5th-year dental students. Data were collected by questionnaires and analyzed by Mann-Whitney U-test and Kruskal-Wallis test using SPSS software version 16 for Windows (SPSS Inc., Chicago, IL, USA). RESULTS: In this study, 235 dental students participated in the study. The average awareness and knowledge score was 7.27 (1.92). Based on the opinion of 57% (134/235) students, hydrogen peroxide was selected as the suitable material for sterilization and 24.6% (58/235) students believed that autoclave sterilization is a good way for the purpose. CONCLUSION: The results of this investigation indicated that awareness and knowledge of undergraduate dental students in relation to sterilization/disinfection methods of extracted human teeth were good. However, deficiencies were observed in relation to teaching the material and methods suitable for sterilization.

4.
J Indian Prosthodont Soc ; 15(2): 91-7, 2015.
Article in English | MEDLINE | ID: mdl-26929493

ABSTRACT

STATEMENT OF PROBLEM: Selection of proper sized maxillary anterior teeth is one of the difficult clinical steps in complete denture esthetics. Several studies have been reported to establish methods of estimating the combined width of maxillary anterior teeth. PURPOSE: The aim of this study was to determine whether a relationship exists between the innercanthal distance (ICD) and interalar width (IAW) with the combined width of maxillary anterior teeth. MATERIAL AND METHODS: The maxillary anterior teeth of 600 adult subjects were examined. ICD was measured between the median angles of the palpebral fissure. IAW was measured between the ala of the nose at their widest point. The mean combined width of the maxillary anterior teeth was determined intraorally at their widest dimension. Pearson's correlation coefficients were calculated to determine the relationship between ICD, IAW and the combined width of maxillary anterior teeth (α =0.05). RESULTS: Although the Pearson correlation coefficients were relatively small, a significant relationship existed between innercanthal dimension and IAW (P < 0001). CONCLUSION: Within the limitations of this study, the results suggest that ICD and IAW cannot be used as a preliminary method for determining the width of the maxillary anterior teeth for edentulous patients.

5.
Geriatr Gerontol Int ; 14(3): 541-8, 2014 Jul.
Article in English | MEDLINE | ID: mdl-24020433

ABSTRACT

AIM: The purpose of this pilot study was to determine if a diagnosis of dementia influenced pain self-reports and pain medication use in a group of verbally communicative nursing home (NH) residents. METHODS: The study design was a between groups, cross-sectional chart audit and a seven-question structured pain interview comparing outcomes in residents with and without a diagnosis of dementia. The study was carried out at a large metropolitan NH in the southern USA. The participants consisted of 52 long-stay NH residents capable of self-consent with at least one order for pain medication (opioid or non-narcotic) either pro re nata, scheduled or both. Approximately 40% (n = 20) had a diagnosis of dementia. RESULTS: Although each group had similar pain-related diagnoses, residents without a dementia diagnosis were significantly more likely to have a medication order for an opioid (OR 4.37,95% CI 1.29-14.73, P = 0.018). Based on self-reported pain interview responses, no statistically significant differences were identified between the groups for chronic pain symptoms. However, among residents who reported current pain, those with a dementia diagnosis reported greater pain intensity (based on a 0-10 numeric rating scale) than did those without dementia (median 8.0 vs 6.0, respectively; P = 0.010). CONCLUSIONS: Verbally communicative NH residents with mild and moderate cognitive impairment can report their pain symptoms and pain intensity. Nurses in long-term care might assume that residents with dementia cannot reliably self-report their pain; however, suffering from untreated severe pain could exacerbate cognitive impairment, worsen functional impairment and severely impair sleep. A brief, focused pain interview might be one method for increasing the detection of moderate to severe pain in verbally communicative NH residents with dementia.


Subject(s)
Analgesics/therapeutic use , Dementia/complications , Drug Utilization , Nursing Homes , Pain/drug therapy , Pain/psychology , Aged , Aged, 80 and over , Case-Control Studies , Cross-Sectional Studies , Dementia/psychology , Female , Humans , Male , Outcome Assessment, Health Care , Pain/diagnosis , Pain Measurement , Pilot Projects , Self Report
6.
Article in English | MEDLINE | ID: mdl-25569941

ABSTRACT

In this work an attempt has been made to enhance the edges and segment the boundary of intima-media layer of Common Carotid Artery (CCA) using anisotropic diffusion filter and level set method. Ultrasound B mode longitudinal images of normal and abnormal images of common carotid arteries are used in this study. The images are subjected to anisotropic diffusion filter to generate edge map. This edge map is used as a stopping boundary in variational level set method without re-initialisation to segment the intima-media layer. Geometric features are extracted from this layer and analyzed statistically. Results show that anisotropic diffusion filtering is able to extract the edges in both normal and abnormal images. The obtained edge maps are found to have high contrast and sharp edges. The edge based variational level set method is able to segment the intima-media layer precisely from common carotid artery. The extracted geometrical features such as major axis and extent are found to be statistically significant in differentiating normal and abnormal images. Thus this study seems to be clinically useful in diagnosis of cardiovascular disease.


Subject(s)
Carotid Artery, Common/diagnostic imaging , Tunica Intima/diagnostic imaging , Algorithms , Cardiovascular Diseases/diagnosis , Humans , Image Processing, Computer-Assisted , Longitudinal Studies , Ultrasonography
7.
Appl Biochem Biotechnol ; 170(1): 149-63, 2013 May.
Article in English | MEDLINE | ID: mdl-23483408

ABSTRACT

Wastewater discharged from cheese industries is often characterized by high values of organic pollutants, solids, and nutrients. An aerated trickling biofilter using peat and perlite as filter media was employed in a pilot-scale level in order to evaluate the performance of biofilter for removal of pollutants from cheese industry wastewater. The biofilter was operated for a period of 33 days under laboratory conditions, and several parameters were monitored. The results showed a significant improvement in the quality of treated effluent. The maximum removal efficiencies of chemical oxygen demand and biological oxygen demand were 99.2 and 99.9 %, respectively. Significant reduction in total suspended solids (>96 %) was also achieved. A stable ammoniacal-nitrogen (NH(4)-N) removal was accompanied by biofilter. On an average, NH(4)-N and total nitrogen decreased by 98.7 and 72 %, respectively, with a significant portion of NH(4)-N being converted to nitrate-nitrogen (NO(3)-N). Also, a molecular approach based on 16S rDNA was employed to analyze the bacterial community composition present in the biofilter. A comparative sequence analysis of excised denaturing gradient gel electrophoresis bands revealed the presence of diverse groups of bacteria belonging to α- and ß-Proteobacteria and Bacteroidetes phylum. We conclude from the results that the use of trickling biofilter is highly effective and a potential treatment method for polishing cheese industry wastewater before being discharged into the local environment.


Subject(s)
Filtration/methods , RNA, Ribosomal, 16S/genetics , Wastewater/chemistry , Water Purification/methods , Ammonia/chemistry , Bacteroides/classification , Bacteroides/genetics , Bacteroides/isolation & purification , Biodegradation, Environmental , Cheese , Filtration/instrumentation , Food Industry , Microbial Consortia/genetics , Oxygen/chemistry , Proteobacteria/classification , Proteobacteria/genetics , Proteobacteria/isolation & purification , RNA, Ribosomal, 16S/classification , Soil , Waste Disposal, Fluid
8.
Environ Manage ; 49(5): 1037-53, 2012 May.
Article in English | MEDLINE | ID: mdl-22476666

ABSTRACT

Increasing phosphorus (P) content and decreasing water quality of Saint-Augustin Lake, Quebec City, Canada, has led to implementation of an Integrated Watershed Management Plan to restore the lake. As a part of the plan, the effects of different restoration techniques on lake water quality and biological community (i.e., biological compatibility) were assessed during an isolated water enclosure study and laboratory microcosm assay, respectively. The restoration techniques include: (i) coagulation of P by alum only (20 mg L(-1)), (ii) active capping of sediments using a calcite layer of 10 cm, and (iii) a complete method involving both alum coagulation and calcite capping. The results showed that the total P (TP) was greatly decreased (76-95 %) by alum + calcite, followed by calcite only (59-84 %). Secchi depth was 106 % greater and chlorophyll a concentrations were declined by 19-78 % in the enclosure which received both alum and calcite. Results of the biological compatibility test showed that total phytoplankton biomass declined by 31 % in microcosms composed of alum + calcite. No significant (P > 0.05) toxic effect was found on the survival of Daphnia magna and Hyalella azteca in both alum only and alum + calcite microcosms. Although the alum + calcite technique impaired the survival of Chironomus riparius, the midge emergence was much higher compared to alum only and control. Overall, the alum + calcite application was effective in controlling P release from sediment and lowering water column P concentrations, and thus improving the water quality and aquatic life of Saint-Augustin Lake. However, the TP concentrations are still higher than the critical limit (20 µg L(-1)) for aquatic life and the water column remained in the eutrophic state even after treatment. Increased TP concentrations, to higher than ambient levels of the lake, in the water column of all four enclosures, due to bioturbation artefact triggered by the platform installation, likely cause insufficient dosages of alum and/or calcite applied and reduced their effectiveness.


Subject(s)
Environmental Monitoring/methods , Environmental Restoration and Remediation/methods , Eutrophication , Lakes/analysis , Water Quality/standards , Alum Compounds/chemistry , Biomass , Calcium Carbonate/chemistry , Ecosystem , Geographic Information Systems , Geologic Sediments/chemistry , Phosphorus/analysis , Phytoplankton/growth & development , Quebec
9.
J Prosthodont ; 21(3): 200-4, 2012 Apr.
Article in English | MEDLINE | ID: mdl-22356269

ABSTRACT

For patients undergoing radical head and neck surgery, the deformity or physical defect adds to the agony. Rehabilitation of patients with such deformities is a challenge for the maxillofacial prosthodontist to enhance the esthetics and give psychological strength to the patient. This clinical report describes the rehabilitation, using a silicone prosthesis, of a large facial and orbital defect due to mucoepidermoid carcinoma.


Subject(s)
Eye, Artificial , Face , Prostheses and Implants , Prosthesis Design , Adult , Biocompatible Materials/chemistry , Carcinoma, Mucoepidermoid/surgery , Humans , Male , Orbital Neoplasms/surgery , Palatal Neoplasms/surgery , Prosthesis Coloring , Silicones/chemistry , Skull Neoplasms/surgery , Zygoma/surgery
10.
Environ Monit Assess ; 183(1-4): 571-9, 2011 Dec.
Article in English | MEDLINE | ID: mdl-21359998

ABSTRACT

Prediction models for exchangeable soil lead, published earlier in this journal (Andra et al. 2010a), were developed using a suite of native lead (Pb) paint-contaminated residential soils from two US cities heavily populated with homes constructed prior to Pb ban in paints. In this study, we tested the feasibility and practical applications of these prediction models for developing a phytoremediation design using vetiver grass (Vetiveria zizanioides), a Pb-tolerant plant. The models were used to estimate the exchangeable fraction of Pb available for vetiver uptake in four lead-spiked soil types, both acidic and alkaline, with varying physico-chemical properties and that are different from those used to build the prediction models. Results indicate a strong correlation for predictable exchangeable Pb with the observed fraction and as well with total Pb accumulated by vetiver grass grown in these soils. The correlation coefficient for the predicted vs. observed exchangeable Pb with p < 0.001 was 0.999, 0.996, 0.949, and 0.998 in the Immokalee, Millhopper, Pahokee Muck, and Tobosa soil type, respectively. Similarly, the correlation coefficient for the predicted exchangeable Pb vs. accumulated Pb in vetiver grass with p < 0.001 was 0.948, 0.983, 0.929, and 0.969 for each soil type, respectively. This study suggests that the success of a phytoremediation design could be assessed upfront by predicting the exchangeable Pb fraction in a given soil type based on its properties. This helps in modifying the soil conditions to enhance phytoextraction of Pb from contaminated soils.


Subject(s)
Soil Pollutants/analysis , Soil/analysis , Biodegradation, Environmental , Lead/analysis , Models, Theoretical
11.
Environ Monit Assess ; 175(1-4): 661-76, 2011 Apr.
Article in English | MEDLINE | ID: mdl-20568011

ABSTRACT

Lead (Pb)-based paints pose a serious health problem to people living in residential settings constructed prior to 1978. Children are at a greater risk to Pb exposure resulting from hand-to-mouth activity in Pb-contaminated residential soils. For soil Pb, the most environmentally friendly, potentially cheap, and visually unobtrusive in situ technology is phytoremediation. However, the limiting factor in a successful phytoremediation strategy is the availability of Pb for plant uptake. The purpose of this study was to establish a relationship between soil properties and the plant-available/exchangeable Pb fraction in the selected Pb-based paint-contaminated residential sites. We selected 20 such sites from two different locations (San Antonio, Texas and Baltimore, Maryland) with varying soil properties and total soil Pb concentrations ranging between 256 and 4,182 mg kg(-1). Despite higher Pb levels in these soils that exceeds US EPA permissible limit of 400 mg kg(-1), it is known that the plant-available Pb pools are significantly lower because of their sorption to soil components such as organic matter, Fe-Mn oxides, and clays, and their precipitation in the form of carbonates, hydroxides, and phosphates. Principal component analysis and hierarchical clustering showed that the potentially plant-available Pb fraction is controlled by soil pH in the case of acidic Baltimore soils, while soil organic matter plays a major role in alkaline San Antonio soils. Statistical models developed suggest that Pb is likely to be more available for plant uptake in Baltimore soils and a chelant-assisted phytoextraction strategy will be potentially necessary for San Antonio soils in mobilizing Pb from complexed pool to the plant-available pool. A thorough knowledge of site-specific factors is therefore essential in developing a suitable and successful phytoremediation model.


Subject(s)
Biodegradation, Environmental , Lead/metabolism , Plants/metabolism , Soil Pollutants/metabolism , Cluster Analysis , Principal Component Analysis
12.
Environ Pollut ; 157(7): 2173-83, 2009 Jul.
Article in English | MEDLINE | ID: mdl-19282075

ABSTRACT

Ethylenediamene tetraacetic acid (EDTA) has been used to mobilize soil lead (Pb) and enhance plant uptake for phytoremediation. Chelant bound Pb is considered less toxic compared to free Pb ions and hence might induce less stress on plants. Characterization of possible Pb complexes with phytochelatins (PCn, metal-binding peptides) and EDTA in plant tissues will enhance our understanding of Pb tolerance mechanisms. In a previous study, we showed that vetiver grass (Vetiveria zizanioides L.) can accumulate up to 19,800 and 3350 mg Pb kg(-1) dry weight in root and shoot tissues, respectively; in a hydroponics set-up. Following the basic incubation study, a greenhouse experiment was conducted to elucidate the efficiency of vetiver grass (with or without EDTA) in remediating Pb-contaminated soils from actual residential sites where Pb-based paints were used. The levels of total thiols, PCn, and catalase (an antioxidant enzyme) were measured in vetiver root and shoot following chelant-assisted phytostabilization. In the presence of 15 mM kg (-1) EDTA, vetiver accumulated 4460 and 480 mg Pb kg(-1) dry root and shoot tissue, respectively; that are 15- and 24-fold higher compared to those in untreated controls. Despite higher Pb concentrations in the plant tissues, the amount of total thiols and catalase activity in EDTA treated vetiver tissues was comparable to chelant unamended controls, indicating lowered Pb toxicity by chelation with EDTA. The identification of glutathione (referred as PC1) (m/z 308.2), along with chelated complexes like Pb-EDTA (m/z 498.8) and PC(1)-Pb-EDTA (m/z 805.3) in vetiver root tissue using electrospray tandem mass spectrometry (ES-MS) highlights the possible role of such species towards Pb tolerance in vetiver grass.


Subject(s)
Chrysopogon/chemistry , Phytochelatins/isolation & purification , Baltimore , Biodegradation, Environmental , Catalase/analysis , Chelating Agents/pharmacology , Chromatography, Liquid , Chrysopogon/metabolism , Cities , Edetic Acid/pharmacology , Hydroponics , Industrial Waste/analysis , Lead/analysis , Plant Roots/chemistry , Plant Roots/metabolism , Plant Shoots/chemistry , Plant Shoots/metabolism , Soil Pollutants/analysis , Sulfhydryl Compounds/analysis , Tandem Mass Spectrometry/methods , Texas
13.
Environ Pollut ; 156(3): 1139-48, 2008 Dec.
Article in English | MEDLINE | ID: mdl-18479792

ABSTRACT

Chelation of metals is an important factor in enhancing solubility and hence, availability to plants to promote phytoremediation. We compared the effects of two chelants, namely, ethylenediaminetetraacetic acid (EDTA) and ethylenediaminedisuccinic acid (EDDS) in enhancing mobilized lead (Pb) in Pb-based paint contaminated residential soils collected from San Antonio, Texas and Baltimore, Maryland. Batch incubation studies were performed to investigate the effectiveness of the two chelants in enhancing mobilized Pb, at various concentrations and treatment durations. Over a period of 1 month, the mobilized Pb pool in the San Antonio study soils increased from 52 mg kg(-1) to 287 and 114 mg kg(-1) in the presence of 15 mM kg(-1) EDTA and EDDS, respectively. Stepwise linear regression analysis demonstrated that pH and organic matter content significantly affected the mobilized Pb fraction. The regression models explained a large percentage, from 83 to 99%, of the total variation in mobilized Pb concentrations.


Subject(s)
Chelating Agents/pharmacology , Environmental Restoration and Remediation/methods , Lead/analysis , Paint , Soil Pollutants/analysis , Biodegradation, Environmental , Edetic Acid/pharmacology , Ethylenediamines/pharmacology , Housing , Humic Substances , Hydrogen-Ion Concentration , Maryland , Metals, Heavy/analysis , Soil/analysis , Succinates/pharmacology , Texas
14.
Colloids Surf B Biointerfaces ; 63(2): 159-63, 2008 Jun 01.
Article in English | MEDLINE | ID: mdl-18206355

ABSTRACT

Water is seriously polluted by the discharge of various industrial wastewater containing heavy metals. Among them, chromium is considered to be toxic to living organisms and it is released mostly from tanneries. The chromium-contaminated water is discharged into nearby water bodies and it affects both aquatic and terrestrial plants. So the present experiment was conducted with an aquatic plant, water lettuce (Pistia stratiotes L.) and a terrestrial plant soybean (Glycine max L. Merr.). They were treated with different concentrations (0, 5, 10, 25, 50, 100 and 200mg/L) of potassium dichromate solution. The biochemical parameters such as total chlorophyll, carotenoid, protein and amino acid content and the enzymatic activities like catalase and peroxidase were estimated. The accumulation of chromium was also analysed in both the plants. All the biochemical contents and enzyme activities of water lettuce and soybean seedlings showed a great variation with respect to the increase in chromium concentrations. The accumulation of chromium increased gradually with the increase of chromium concentrations. Total inhibition of all the parameters were observed at 300 mg/L chromium concentration. The terrestrial plant soybean was sensitive than the aquatic plant water lettuce towards chromium stress.


Subject(s)
Araceae/drug effects , Chromium/toxicity , Glycine max/drug effects , Water Pollutants, Chemical/toxicity , Araceae/enzymology , Araceae/metabolism , Catalase/metabolism , Peroxidases/metabolism , Photosynthesis , Pigments, Biological , Plant Proteins/metabolism , Glycine max/enzymology , Glycine max/metabolism
15.
Nucleic Acids Res ; 34(Web Server issue): W128-32, 2006 Jul 01.
Article in English | MEDLINE | ID: mdl-16844975

ABSTRACT

3dSS is a web-based interactive computing server, primarily designed to aid researchers, to superpose two or several 3D protein structures. In addition, the server can be effectively used to find the invariant and common water molecules present in the superposed homologous protein structures. The molecular visualization tool RASMOL is interfaced with the server to visualize the superposed 3D structures with the water molecules (invariant or common) in the client machine. Furthermore, an option is provided to save the superposed 3D atomic coordinates in the client machine. To perform the above, users need to enter Protein Data Bank (PDB)-id(s) or upload the atomic coordinates in PDB format. This server uses a locally maintained PDB anonymous FTP server that is being updated weekly. This program can be accessed through our Bioinformatics web server at the URL http://cluster.physics.iisc.ernet.in/3dss/ or http://10.188.1.15/3dss/.


Subject(s)
Software , Structural Homology, Protein , Computer Graphics , Databases, Protein , Internet , Models, Molecular , Phospholipases A/chemistry , Water/chemistry
16.
Nucleic Acids Res ; 33(Web Server issue): W85-8, 2005 Jul 01.
Article in English | MEDLINE | ID: mdl-15980587

ABSTRACT

FF (Fragment Finder) is a web-based interactive search engine developed to retrieve the user-desired similar 3D structural fragments from the selected subset of 25 or 90% non-homologous protein chains. The search is based on the comparison of the main chain backbone conformational angles (phi and ). Additionally, the queried motifs can be superimposed to find out how similar the structural fragments are, so that the information can be effectively used in molecular modeling. The engine has facilities to view the resultant superposed or individual 3D structure(s) on the client machine. The proposed web server is made freely accessible at the following URL: http://cluster.physics.iisc.ernet.in/ff/ or http://144.16.71.148/ff/.


Subject(s)
Amino Acid Motifs , Software , Internet , Molecular Structure , Proteins/chemistry
17.
Acta Crystallogr D Biol Crystallogr ; 61(Pt 5): 634-6, 2005 May.
Article in English | MEDLINE | ID: mdl-15858275

ABSTRACT

The Secondary Structural Elements of Proteins (SSEP) database is an integrated and comprehensive knowledge base for accessing information related to all the secondary-structural elements present in non-redundant (25 and 90%) protein chains. The new version 2.0 of the database contains 2485 and 8595 protein chains from the 25 and 90% non-redundant data sets, respectively. The necessary web interfaces have been developed that enable users to visualize the three-dimensional structure of the secondary-structural element in the client machine using the free molecular-visualization program RASMOL. This source is updated at regular intervals and can be accessed through the bioinformatics web server at the URL http://cluster.physics.iisc.ernet.in/ssep or http://144.16.71.148/ssep/.


Subject(s)
Artificial Intelligence , Protein Structure, Secondary , Proteins/chemistry , Computational Biology , Crystallography, X-Ray , Models, Molecular , Quality Control , Temperature
18.
Bioresour Technol ; 96(3): 309-16, 2005 Feb.
Article in English | MEDLINE | ID: mdl-15474931

ABSTRACT

Biological wastes (sawdust, rice husk, coirpith and charcoal) and a naturally occurring mineral (vermiculite) have been tested for their effectiveness in removing Cr from tannery effluent through batch and column experiments. The adsorption capacities of the substrates were also evaluated using isotherm tests and computing distribution co-efficient. The sawdust exhibited a higher adsorption capacity (k = 1482 mgkg(-1)), followed by coirpith (k = 159 mgkg(-1)). The biosorbent and mineral vermiculite in columns were found very effective in removing Cr from tannery effluent. About 94% removal of Cr was achieved by a column of coirpith, and equally (93%) by a column containing a mixture of coirpith and vermiculite. This study showed that biological wastes are potential adsorbents of Cr, which could be successfully used to reduce the Cr concentrations in tannery effluent.


Subject(s)
Aluminum Silicates/chemistry , Chromium/chemistry , Tanning , Waste Disposal, Fluid/methods , Adsorption , Charcoal , Spectrophotometry, Ultraviolet , Waste Disposal, Fluid/instrumentation , Waste Products , Wood
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