ABSTRACT
OBJECTIVE: To study the effect of Ginkgo biloba exocarp polysaccharides (GBEP) on HL-60 cells in vitro. METHOD: Cell culture, MTT, flow cytometry were performed to observe proliferation, apoptosis and changes of relevant gene expression of HL-60 cells. RESULT: GBEP (40-160 mg/L, 48 h) could inhibit HL-60 cells proliferation and the expression of proliferation-promotion gene c-myc, induce HL-60 cells apoptosis and down-regulate the expression of apoptosis-inhibitory gene bcl-2. CONCLUSION: Influence of GBEP on HL-60 cells proliferation and apoptosis may relate to its effects on the expression of proliferation-promotion gene c-myc and apoptosis-inhibitory gene bcl-2.
Subject(s)
Apoptosis/drug effects , Drugs, Chinese Herbal/pharmacology , Ginkgo biloba/chemistry , Polysaccharides/pharmacology , Cell Division/drug effects , Drugs, Chinese Herbal/chemistry , Drugs, Chinese Herbal/isolation & purification , Flow Cytometry , HL-60 Cells , Humans , Plant Bark/chemistry , Polysaccharides/isolation & purification , Proto-Oncogene Proteins c-bcl-2/biosynthesis , Proto-Oncogene Proteins c-bcl-2/genetics , Proto-Oncogene Proteins c-myc/biosynthesis , Proto-Oncogene Proteins c-myc/genetics , Seeds/chemistry , Tumor Cells, CulturedABSTRACT
AIM: To study the therapeutic mechanism of Ginkgo biloba exocarp polysaccharides (GBEP) on gastric cancer. METHODS: Thirty patients with gastric cancer were treated with oral GBEP capsules. The area of tumors was measured by electron gastroscope before and after treatment, then the inhibitory and effective rates were calculated. The ultrastructures of tumor cells were examined by transmissional electron microscope. Cell culture, MTT, flow cytometry were performed to observe proliferation, apoptosis and changes of relevant gene expression of human gastric cancer SGC-7901 cells. RESULTS: Compared with the statement before treatment, GBEP capsules could reduce the area of tumors, and the effective rate was 73.4%. Ultrastructural changes of the cells indicated that GBEP could induce apoptosis and differentiation in tumor cells of patients with gastric cancer. GBEP could inhibit the growth of human gastric cancer SGC-7901 cells following 24-72 h treatment in vitro at 10-320 mg/L, which was dose- and time-dependent. GBEP was able to elevate the apoptosis rate and expression of c-fos gene, but reduce the expression of c-myc and bcl-2 genes also in a dose-dependent manner. CONCLUSION: The therapeutic mechanism of GBEP on human gastric cancer may relate to its effects on the expression of c-myc, bcl-2 and c-fos genes, which can inhibit proliferation and induce apoptosis and differentiation of tumor cells.
