Retraction Note: Long non-coding RNA DLEU7-AS1 promotes the occurrence and development of colorectal cancer via Wnt/ß-catenin pathway.

The article "Long non-coding RNA DLEU7-AS1 promotes the occurrence and development of colorectal cancer via Wnt/ß-catenin pathway", by X.-B. Liu, C. Han, C.-Z. Sun, published in Eur Rev Med Pharmacol Sci 2018; 22 (1): 110-117-DOI: 10.26355/eurrev_201801_14107-PMID: 29364477 has been retracted by the authors as the data cannot be reproduced by further research. This paper has been questioned on PubPeer (https://pubpeer.com/publications/AC72791669051B50085FEC00D0EF8F). In particular, concerns were raised about Table I and the originality of Figures 3A and 3C. The corresponding author states some data cannot be repeated by further research due to some inaccuracies and therefore decided to withdraw the manuscript. This article has been retracted. The Publisher apologizes for any inconvenience this may cause. https://www.europeanreview.org/article/14107.

[Effect of IL-11 on the invasive and metastatic abilities of anaplastic thyroid carcinoma cells].

Objective: To explore the effect of hematopoietic cytokines IL-11 on invasion and metastasis abilities of anaplastic thyroid cacinoma(ATC) cells. Methods: Real-time PCR was performed for examining the IL-11 mRNA expression in thyroid carcinoma cell lines, and IL-11 protein expression in the supernament of thyroid carcinoma cell lines was detected by ELISA. Molecular cloning was employed to construct IL-11 stable knockdown cell line; MTT assay was used to analyze the effect of IL-11 on the proliferation of ATC cells; Transwell and wound healing assays were employed to analyze the abilities of migration and invasion in ATC cells. Western blotting was used to detect the relative pathway proteins. SPSS statistical package 19.0 was used to analyze the date, and Student's t test was used for multiple comparisons. Results: The protein level of IL-11 were significantly lower in knock-down cell lines than that in negative control cell lines(21.55±1.69, 16.18±0.85, 26.37±2.00 vs 54.54±3.99, all P<0.05). Colony formation assays reveal that colony number between knock-down cells and negative control cells has no significance(P>0.05). Meanwhile, MTT assays show that there is no significance between knock-down cell lines and negative control cell line(P>0.05). However, Transwell invasion and migration assays show that number of migrated cells is increased when ATC cells were treated with rhIL-11(0-100 ng/ml)at increasing concentrations. Conclusion: IL-11 improves the migratory and invasive abilities of ATC cells via inducing EMT of ATC cells, and it can be used as a potential target for ATC molecular targeted therapy.

Long non-coding RNA DLEU7-AS1 promotes the occurrence and development of colorectal cancer via Wnt/ß-catenin pathway.

OBJECTIVE: To investigate the expression features of long non-coding RNA (lncRNA) DLEU7-AS1 in colorectal cancer (CRC), so as to further study its role in the occurrence and development of CRC and its potential regulatory mechanism. PATIENTS AND METHODS: The expression levels of lncRNA DLEU7-AS1 in 82 pairs of CRC tissues and para-carcinoma normal tissues were detected via quantitative Real-time polymerase chain reaction (qRT-PCR), and the correlation of DLEU7-AS1 expression with pathological indexes of CRC and patients' prognosis was analyzed. Besides, the expression of DLEU7-AS1 in CRC cells was further detected via qRT-PCR. The DLEU7-AS1 knockdown expression model was established using small interfering RNA in CRC cell lines HT-29 and HCT-116, and the effect of DLEU7-AS1 on biological functions of CRC cells was analyzed via Cell Counting Kit-8 (CCK-8) and transwell invasion/migration assay. Finally, its potential mechanism was investigated via Western blotting. RESULTS: The results of qRT-PCR showed that the expression level of DLEU7-AS1 in CRC was significantly higher than that in normal tissues, and the difference was statistically significant. Compared with those in patients with low DLEU7-AS1 expression, the tumor stage in patients with high DLEU7-AS1 expression was higher, the prevalence rates of lymph node metastasis and distant metastasis were higher, and the overall survival rate was lower. Compared with those in the negative control group, the cell proliferation, invasion, and migration capacities were decreased significantly in DLEU7-AS1 knockdown expression group. Moreover, the results of Western blotting revealed that the expressions of key proteins in Wnt/ß-catenin pathway, including ß-catenin, c-myc, and cyclinD1, were decreased in si-DLEU7-AS1. CONCLUSIONS: The expression of DLEU7-AS1 is significantly increased in CRC, which is markedly associated with CRC staging, lymph node metastasis, distant metastasis and poor prognosis. DLEU7-AS1 may promote the proliferation, invasion and migration capacities of CRC through regulating the Wnt/ß-catenin pathway.

[Effect of concentrated growth factors on the treatment of degree II furcation involvements of mandibular molars].

OBJECTIVE: Concentrated growth factors (CGF), a new generation of platelet concentrate products, appears to have more abundant growth factors because of its special centrifugation process. However, there are few studies supporting this. This study was designed to evaluate the effect of CGFs in the treatment of II° furcations of mandibular molars. METHODS: In the present study, thirty-one II° furcation involvements in twenty mandiblular molars were included and randomly divided into two groups. The furcation involvements in the experimental group were treated with bone graft therapy combined with CGFs, and the furcation involvements in the control group were treated with bone graft therapy alone. The clinical examination and cone beam computed tomography (CBCT) were performed at baseline and 1 year post-surgery for the two groups. The changes of clinical and CBCT data at baseline and 1 year post-surgery were compared between the experimental group and the control group. RESULTS: At baseline, there were no significant differences between the two groups in the probing depth (PD), vertical clinical attachment loss (CAL-V) and horizontal clinical attachment loss (CAL-H): PD (7.36±2.32) mm (the experimental group) vs. (7.53±2.06) mm (the control group); CAL-V (8.69±1.65) mm (the experimental group) vs. (8.81±1.53) mm (the control group); CAL-H (5.24±2.01) mm (the experimental group) vs. (5.35±2.14) mm (the control group). At the end of 1 year post-surgery, the clinical parameters of both groups were significantly improved (P<0.001). For the experimental group, the average vertical attachment gain was (2.78±1.66) mm, and the vertical attachment loss was improved significantly compared with the baseline (P<0.001); the average horizontal attachment gain was (2.10±1.89) mm, and the horizontal attachment loss were improved significantly compared with the baseline (P<0.001). Furthermore, the improvement degree of the experimental group was significantly higher than that of the control group (P<0.001). At baseline, there were no statistical differences in the vertical bone loss (BL-V) or horizontal bone loss (BL-H) between the two groups (P>0.05): BL-V (5.08±2.17) mm (the experimental group) vs. (5.84±2.65) mm (the control group); BL-H (5.85±2.13) mm (the experimental group) vs. (6.01±2.27) mm (the control group). At 1 year post-surgery, both groups showed significant radiographic bone gain at vertical and horizontal directions compared with baseline (P<0.001). For the experimental group, the average vertical radiographic bone gain was (2.20±1.98) mm, the horizontal radiographic bone gain was (2.51±2.18) mm, the vertical and horizontal radiographic bone loss were both significantly reduced compared with the baseline (P<0.001). For the control group, the average vertical radiographic bone gain was (1.89±2.15) mm, the horizontal radiographic bone gain was (1.30±2.47) mm, the vertical and horizontal radiographic bone losses were both significantly reduced compared with the baseline (P<0.001). And the experimental group showed significantly higher bone gain at vertical and horizontal directions compared with the control group (P<0.001). CONCLUSION: Within the limitation of the present study, CGFs showed positive role in the treatment of II° furcation involvements of mandibular molars.

Triglyceride to high-density lipoprotein cholesterol ratio predicts worse outcomes after acute ischaemic stroke.

BACKGROUND AND PURPOSE: The effect of the triglyceride (TG) to high-density lipoprotein cholesterol (HDL-C) ratio (TG/HDL-C) on clinical outcomes of acute ischaemic stroke (AIS) patients is unclear. This study sought to determine whether the TG/HDL-C ratio in AIS patients is associated with worse outcomes at 3 months. METHODS: Acute ischaemic stroke patients who were admitted from 2011 to 2014 were enrolled in this study. TG, total cholesterol (TC), HDL-C and low-density lipoprotein cholesterol (LDL-C) were collected on admission. Three end-points were defined according to the modified Rankin scale (mRS) score at 3 months after symptom onset (excellent outcome, mRS 0-1; good outcome, mRS 0-2; and death, mRS 6). RESULTS: In all, 1006 patients were included (median age 68.5 years; 58.2% male). Higher TG, non-HDL-C and TG/HDL-C were strongly associated with the three end-points after adjustments: excellent [odds ratio (OR) = 1.39, OR 1.89 and OR 2.34, respectively] and good (OR 1.48, OR 2.90 and OR 4.12) outcomes, and death (OR 0.59, OR 0.29 and OR 0.26). According to receiver operating characteristic (ROC) analysis, the best discriminating factor was a TG/HDL-C ≥ 0.87 for excellent outcomes [area under the ROC curve (AUC) 0.596; sensitivity 73.3%; specificity 42.7%] and non-death (AUC 0.674; sensitivity 67.8%; specificity 60.6%) as well as a TG/HDL-C ≥ 1.01 for a good outcome (AUC 0.652; sensitivity 61.6%; specificity 63.2%). Patients with a TG/HDL-C < 0.87 had a 2.94-fold increased risk of death (95% confidence interval 1.89-4.55) compared with patients with a TG/HDL-C ≥ 0.87. CONCLUSIONS: A lower TG/HDL-C was independently associated with death and worse outcome at 3 months in AIS.

[Accuracy analysis of cone beam computed tomography in assessing furcation involvements of mandibular molars].

OBJECTIVE: To evaluate the accuracy of cone beam computed tomography (CBCT) in assessing furcation involvements of mandibular molars. METHODS: In the study, 38 furcation involvements of 22 mandibular molars which would accept furcation surgery were included. Pre-surgical examination, CBCT and periapical radiograph were performed. Then, intra-surgical furcation assessments were compared with those data. RESULTS: Intra-surgical findings confirmed 86.8% of the CBCT data, with a weighed kappa of 0.976. Of the four parameters tested of detailed root anatomy and furcation morphology, the vertical bone loss, the mesial-distal bone loss and the length of the root trunk revealed by CBCT were consistent with their respective intra-surgical values (P>0.05): about vertical bone loss, CBCT was (4.03±2.27) mm, but in surgery was (4.32±2.31) mm; about mesial-distal bone loss, CBCT was (2.05±0.89) mm, but in surgery was (2.07±0.97) mm; about length of the root trunk, CBCT was (2.35±0.88) mm, but in surgery was (2.24± 0.75) mm. The horizontal bone loss revealed by CBCT [(3.40±1.27) mm] was significantly smaller than that by surgery [(3.72±1.19) mm]. However, the mean difference was only 0.32 mm. Periapical radiograph could only obtain vertical bone loss [(2.17±1.32) mm] and mesial-distal bone loss [(1.46± 0.88) mm], which was significantly different from that obtained in surgery. CONCLUSION: CBCT proved high accuracy in assessing the furcation involvement of mandibular molars.

Treating sewage using coimmobilized system of Chlorella pyrenoidosa and activated sludge.

Chlorella pyrenoidosa was coimmobilized with activated sludge to produce algae-bacteria beads for sewage treatment. Hydrolysis/acidogenesis pretreatment could improve the symbiotic microenvironment of coimmobilized Chlorella pyrenoidosa and activated sludge, and as a result, promote the removal of nutrients (COD(cr), inorganic nitrogen and inorganic phosphorus) in the sewage. A photo-bioreactor combining hydrolysis/acidogenesis pretreatment and coimmobilized technique was designed to treat sewage continuously. The results show that, the removal efficiencies of COD(cr), NH4(+)-N and TP reached steady state after 4-days of experiment. The removal efficiencies of COD(cr), NH4(+)-N and TP were 59.6%, 59.0% and 60.3% respectively.

Use of serum and vaccine in combination for prophylaxis following exposure to rabies.

Persons seriously wounded by rabid animals in China were administered postexposure rabies treatment with serum and vaccine. Some of these exposures were similar to those described in an attack by a rabid wolf in Iran in 1954, in which some persons developed rabies. Postexposure treatment consisted of the administration of primary hamster kidney cell rabies vaccine and purified equine antirabies serum. Persons with severe wounds from the bites of rabid animals survived without sequelae when the serum and vaccine were administered together within 3 days of the bite, and persons with superficial exposures survived after treatment with vaccine alone. Serum neutralizing antibodies were present 1 year after the patients had received a full course of treatment. Some persons with severe wounds who had not received antiserum and vaccine died of rabies.