Study on Emission Spectrum of OH Radicals in a Combination System of Pulsed Discharge Plasma and Activated Carbon.

Based on the higher oxidation potential of OH radicals (2.8 V), the synergetic effect of pulsed discharge plasma (PDP) and activated carbon (AC) and the advantages of emission spectroscopic detection, such as easy operation, high accuracy and high sensitivity, the relative emission spectra of the OH radicals generated in the PDP/AC system with oxygen flow were measured by the emission spectroscopic detection technique and the spectral intensity of the OH radicals was used to represent the relative amount of the OH radicals formed in the reaction system. The effect of additive amount of the AC, peak pulse voltage and electrode gap on the relative emission spectrum intensities of OH radicals were investigated to illustrate the crucial factors for the OH radicals formation in the PDP/AC system. In addition, the formation of OH radicals in the two liquid phases of distilled water and acid orange 7 (AO7) solution in the sole PDP system and the PDP/AC system were investigated to testify the synergetic mechanism of PDP/AC and the oxidization of OH radicals on the organic compounds in the reaction system. The obtained results showed that the catalytic effect of the AC increased with the increase of the additive amount of the AC in the PDP system, which led to the increase of the relative emission spectral intensities of the formed OH radicals in the synergistic system; higher peak pulse voltage was in favor of the energy input in the discharge system and then enhanced the formation of OH radicals; increase of the electrode gap led to the decrease of energy efficiency in the reaction system and the decrease of the formed OH radicals in the PDP/AC system; the formation of OH radicals in the PDP/AC system was higher than that in the sole PDP system both in the distilled water and in the AO7 solution; the formation of OH radicals in the distilled water was higher than those in the AO7 solution no matter the reaction system was the sole PDP system or the PDP/AC system. The two results indicated that the AC addition was beneficial to the formation of OH radicals in the PDP system and the OH radicals had an important effect on the organic compounds degradation both in the sole PDP system and in the PDP/AC system.

Selective recognition and elimination of nicotinic acetylcholine receptor-reactive B cells by a recombinant fusion protein AChR-Fc in myasthenia gravis in vitro.

AChR-reactive B cells play a key role in the pathogenesis of myasthenia gravis (MG) by producing autoantibodies. Selective elimination of AChR-reactive B cells will be a promising way to treat MG. Thus, we generated a fusion protein (referred to as AChR-Fc) composed of the human extracellular domain of AChR α1 subunit and the Fc domain of the human IgG1 heavy chain, which could bind both to AChR-reactive BCR and FcγRIIB on the surface of AChR-reactive B cells. Our results showed that AChR-Fc inhibited the proliferation of AChR-specific hybridoma cells, promoted their apoptosis, and mediated cytotoxicity by cross-linking effector cells and complement. Likewise, AChR-Fc significantly reduced the number of AChR-reactive B cells from spleen of Lewis rats immunized with AChR ex vivo.