Surface weak scratch detection for optical elements based on a multimodal imaging system and a deep encoder-decoder network.

The detection of surface weak scratches is an intractable but vital task in optics-centered industries. However, the intrinsic characteristics of weak scratches, such as a narrow width, long span, and shallow depth, make it extremely difficult to effectively detect these scratches. In this paper, we tackle this issue from two perspectives. First, a multimodal microscopic imaging system is created by combining discrete multispectral illumination with linear polarization. Imaging experiments demonstrated that this system could highlight more scratch details, improve image clarity, and alleviate the image blur problem induced by wide spectrum scattered lights. Second, a scratch-oriented U-shaped deep encoder-decoder network equipped with optimized residual encoding modules, serial-parallel multiscale fusion modules, and triple-convolution decoding modules is proposed to segment the weak scratches from a raw image. The detection experiments demonstrate that our model can accurately segment the weak scratches on optical surfaces and achieve better detection performance using significantly fewer parameters compared to similar deep learning models. Meanwhile, experiments on the building crack dataset prove the excellent generalization capability.

Study on a new type of environment-friendly polymer and its preliminary application as soil consolidation agent during tree transplanting.

Transplanting trees with rhizospheric soil is an important way to facilitate tree survival in the process of landscaping and reforestation. Traditional way to prevent looseness of rhizospheric soil is forming soil balls around the roots with bags, boxes or rope wrapping, which is cumbersome, laborious and easy to break. This study is aimed to develop a new type of degradable environment-friendly polymer as soil consolidation agent to facilitate tree transplanting. In this paper, the KGM/CA/PVA ternary blending soil consolidation agent was prepared by using Konjac glucomannan (KGM), chitosan (CA) and polyvinyl alcohol (PVA) as raw materials. Through the verification and evaluation, the clay and sandy soil can be consolidated and formed into soil balls by the ternary blend adhesive, which was convenient for transportation. The preliminary application of the ternary blend adhesive in the transplanting process of sierra salvia, Japanese Spindle (Euonymus japonicus) and Juniperus sabina 'Tamaricifolia' confirmed that the application of soil consolidation agent can effectively solve the problem that the root ball of seedling is easily broken in the process of transplant. And the application of soil consolidation agent has no adverse effect on the growth of transplanted seedlings. The research and development of ternary blending soil consolidation agent and its preliminary application in seedling transplanting will provide a new solution to solve the problem of soil ball breakage in the process of seedling transplanting. This is an important stage in the development of new seedling transplanting technology. Therefore, the research and development of soil consolidation agent is of great significance.

Comparative Transcriptome Analysis Reveals Stem Secondary Growth of Grafted Rosa rugosa 'Rosea' Scion and R. multiflora 'Innermis' Rootstock.

Grafted plant is a chimeric organism formed by the connection of scion and rootstock through stems, so stem growth and development become one of the important factors to affect grafted plant state. However, information regarding the molecular responses of stems secondary growth after grafting is limited. A grafted Rosa plant, with R. rugosa 'Rosea' as the scion (Rr_scion) grafted onto R. multiflora 'Innermis' as the stock (Rm_stock), has been shown to significantly improve stem thickness. To elucidate the molecular mechanisms of stem secondary growth in grafted plant, a genome-wide transcription analysis was performed using an RNA sequence (RNA-seq) method between the scion and rootstock. Comparing ungrafted R. rugosa 'Rosea' (Rr) and R. multiflora 'Innermis' (Rm) plants, there were much more differentially expressed genes (DEGs) identified in Rr_scion (6887) than Rm_stock (229). Functional annotations revealed that DEGs in Rr_scion are involved in two Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways: the phenylpropanoid biosynthesis metabolism and plant hormone signal transduction, whereas DEGs in Rm_stock were associated with starch and sucrose metabolism pathway. Moreover, different kinds of signal transduction-related DEGs, e.g., receptor-like serine/threonine protein kinases (RLKs), transcription factor (TF), and transporters, were identified and could affect the stem secondary growth of both the scion and rootstock. This work provided new information regarding the underlying molecular mechanism between scion and rootstock after grafting.

A Stress-Associated Protein, PtSAP13, From Populus trichocarpa Provides Tolerance to Salt Stress.

The growth and production of poplars are usually affected by unfavorable environmental conditions such as soil salinization. Thus, enhancing salt tolerance of poplars will promote their better adaptation to environmental stresses and improve their biomass production. Stress-associated proteins (SAPs) are a novel class of A20/AN1 zinc finger proteins that have been shown to confer plants' tolerance to multiple abiotic stresses. However, the precise functions of SAP genes in poplars are still largely unknown. Here, the expression profiles of Populus trichocarpa SAPs in response to salt stress revealed that PtSAP13 with two AN1 domains was up-regulated dramatically during salt treatment. The ß-glucuronidase (GUS) staining showed that PtSAP13 was accumulated dominantly in leaf and root, and the GUS signal was increased under salt condition. The Arabidopsis transgenic plants overexpressing PtSAP13 exhibited higher seed germination and better growth than wild-type (WT) plants under salt stress, demonstrating that overexpression of PtSAP13 increased salt tolerance. Higher activities of antioxidant enzymes were found in PtSAP13-overexpressing plants than in WT plants under salt stress. Transcriptome analysis revealed that some stress-related genes, including Glutathione peroxidase 8, NADP-malic enzyme 2, Response to ABA and Salt 1, WRKYs, Glutathione S-Transferase, and MYBs, were induced by salt in transgenic plants. Moreover, the pathways of flavonoid biosynthesis and metabolic processes, regulation of response to stress, response to ethylene, dioxygenase activity, glucosyltransferase activity, monooxygenase activity, and oxidoreductase activity were specially enriched in transgenic plants under salt condition. Taken together, our results demonstrate that PtSAP13 enhances salt tolerance through up-regulating the expression of stress-related genes and mediating multiple biological pathways.

Effects of drought and salt-stresses on gene expression in Caragana korshinskii seedlings revealed by RNA-seq.

BACKGROUND: Drought and soil salinity are major abiotic stresses. The mechanisms of stress tolerance have been studied extensively in model plants. Caragana korshinskii is characterized by high drought and salt tolerance in northwestern China; unique patterns of gene expression allow it to tolerate the stress imposed by dehydration and semi-desert saline soil. There have, however, been no reports on the differences between C. korshinskii and model plants in the mechanisms underlying their drought and salt tolerance and regulation of gene expression. RESULTS: Three sequencing libraries from drought and salt-treated whole-seedling- plants and the control were sequenced to investigate changes in the C. korshinskii transcriptome in response to drought and salt stresses. Of the 129,451 contigs, 70,662 (54.12 %) were annotated with gene descriptions, gene ontology (GO) terms, and metabolic pathways, with a cut-off E-value of 10(-5). These annotations included 56 GO terms, 148 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, and 25 Clusters of Orthologous Groups (COG). On comparison of the transcriptomes of the control, drought- and salt-treated plants, 1630 and 1521 contigs showed significant differences in transcript abundance under drought and salt stresses. Compared to the differentially expressed genes (DEGs) in drought- or salt-treated Arabidopsis in the database, 542 DEGs in drought-treated C. korshinskii and 529 DEGs in salt-treated samples were presumably unique to C. korshinskii. The transcription profiles revealed that genes related to transcription factors, protein kinases, and antioxidant enzymes are relevant to the tolerance of drought and salt stress in this species. The expression patterns of 38 randomly selected DEGs were confirmed by quantitative real-time PCR and were essentially consistent with the changes in transcript abundance identified by RNA-seq. CONCLUSIONS: The present study identified potential genes involved in drought and salt tolerance in C. korshinskii, as well as many DEGs uniquely expressed in drought- or salt-treated C. korshinskii samples compared to Arabidopsis. To our knowledge, this study is the first exploration of the C. korshinskii transcriptome under drought and salt conditions, and these results will facilitate the discovery of specific stress-resistance-related genes in C. korshinskii, possibly leading to the development of novel plant cultivars through genetic engineering.