ABSTRACT
Seed treatment with fungicides has been regarded as a principal, effective, and economic technique for soybean [Glycine max (L.) Merr.] against pathogenic microorganisms during seed germination and seedling growth. Investigation of the characteristics of seed-treatment reagents is an indispensable basis for their application. The aim of the present work is to evaluate the use of pyraclostrobin as an ingredient for soybean seed treatment by investigating its accumulation-dissipation kinetics in plants, plant-growth activation, and protection against Phytophthora sojae. The results showed that the pyraclostrobin stimulated the visible growth (root and shoot length) of soybean plants, increased the chlorophyll level and root activity, and lowered the malonaldehyde (MDA) level. The peak level and bioavailability of pyraclostrobin in soybean roots were 19.9- and 33.2-fold those in leaves, respectively, indicating that pyraclostrobin was mainly accumulated in roots. Pyraclostrobin had a continuous positive effect on the flavonoid levels and the phenylalanine ammonialyase (PAL) activity in roots and leaves, which could enhance the plant defense system. Pyraclostrobin showed in vitro toxicity to P. sojae with a half-inhibition concentration (EC50) of 1.59 and 1.24 µg/mL for pyraclostrobin and pyraclostrobin plus salicylhydroxamic acid (SHAM, an inhibitor of the alternative pathway of respiration), respectively. Seed treatment with pyraclostrobin significantly reduced the severity of Phytophthora root rot, with a control efficacy of 60.7%. To the best of our knowledge, this is the first report on the characteristics of pyraclostrobin used in soybean seed treatment and its efficacy against Phytophthora root rot.
Subject(s)
Fungicides, Industrial/pharmacology , Glycine max/metabolism , Glycine max/microbiology , Phytophthora/drug effects , Plant Diseases/prevention & control , Strobilurins/pharmacology , Flavonoids/metabolism , Fungicides, Industrial/chemistry , Gene Expression Regulation, Plant , Kinetics , Phenylalanine Ammonia-Lyase/genetics , Phenylalanine Ammonia-Lyase/metabolism , Phytophthora/physiology , Plant Diseases/genetics , Plant Diseases/microbiology , Plant Proteins/genetics , Plant Proteins/metabolism , Seeds/chemistry , Seeds/growth & development , Seeds/metabolism , Seeds/microbiology , Glycine max/chemistry , Glycine max/growth & development , Strobilurins/chemistryABSTRACT
Winter is the key period for the control of apple diseases, and fungicides are needed to protect the trunk or main branches. Fungicide residue in apple tree bark is an important basis for the action of the pesticide, but there are no reports on analytical methods or dissipation patterns. In this work, thiophanate-methyl, carbendazim, tebuconazole and pyraclostrobin were selected as typical fungicides and a new QuEChERS-HPLC-VWD(QuEChERS extraction followed by high-performance liquid chromatography detection with a variable wavelength detector) analytical method was developed to estimate their residue kinetics in apple tree bark during the winter months. In the pretreatment step, the sorbent for the clean-up of extracts was optimized as 60 mg/ml primary secondary amine and a gradient-elution model followed by a variable wavelength detection was developed for instrumental analysis. Then this method was validated and applied to the analysis of apple tree bark samples with the linearity range of 0.010-50.00 mg/L, quantification limit range of 0.028-0.080 mg/kg and recovery range of 86.1-101.4%. The dissipation kinetics of thiophanate-methyl and pyraclostrobin could be described by the first-order and two-phase kinetics models, respectively. For carbendazim and tebuconazole, two new models were developed to describe their residue kinetics.
