Construction of quaternary ammonium chitosan-coated protein nanoparticles as novel delivery system for curcumin: Characterization, stability, antioxidant activity and bio-accessibility.

This research aimed to develop a novel, effective, and stable delivery system based on zein (ZE), sodium caseinate (SC), and quaternary ammonium chitosan (HACC) for curcumin (CUR). The pH-driven self-assembly combined with electrostatic deposition methods were employed to construct CUR-loaded ZE-SC nanoparticles with HACC coating (ZE-SC@HACC). The optimized nanocomposite was prepared at ZE:SC:HACC:CUR mass ratios of 1:1:2:0.1, and it had encapsulation efficiency of 89.3%, average diameter of 218.2 nm, and ζ-potential of 40.7 mV. The assembly of composites and encapsulation of CUR were facilitated primarily by hydrophobic, hydrogen-bonding, and electrostatic interactions. Physicochemical stability analysis revealed that HACC coating dramatically enhanced ZE-SC nanoparticles' colloidal stability and CUR's resistance to chemical degradation. Additionally, antioxidant activity and simulated digestion results indicated that CUR-ZE-SC@HACC nanoparticles showed higher free radical scavenging capacity and bio-accessibility of CUR than CUR-ZE-SC nanoparticles and free CUR. Therefore, the ZE-SC@HACC nanocomposite is an effective and viable delivery system for CUR.

Bacterial Changes in Boiled Crayfish between Different Storage Periods and Characterizations of the Specific Spoilage Bacteria.

This study investigated changes in the microbial compositions of crayfish tails during storage at 4 °C (for 0-12 days) as measured using high-throughput sequencing (HTS). The specific spoilage organisms (SSOs) in the crayfish tails were isolated using culture-dependent cultivation methods, and they were identified by 16S rRNA and characterized for their enzymatic spoilage potentials (e.g., protease, lipase, phospholipase, and amylase). The spoilage abilities of the selected strains in the crayfish tails were assessed by inoculating them into real food. Moreover, the microbial growth and the volatile basic nitrogen (TVB-N) changes were monitored during the storage period. The results from the HTS showed that the dominant genus of shrimp tails evolved from Streptococcus (D0) to Pseudomonas (D4) and, finally, to Paenisporosarcina (D12) during storage. Seven bacterial species (Acinetobacter lwoffii, Aeromonas veronii, Kurthia gibsonii, Pseudomonas sp., Exiguobacterium aurantiacum, Lelliottia amnigena, and Citrobacter freundii) were screened from the spoiled shrimp tails by the culture-dependent method, among which Aeromonas veronii had the strongest spoilage ability.

The combined effects of ultrasound and plasma-activated water on microbial inactivation and quality attributes of crayfish during refrigerated storage.

In this study, a decontamination technology combining ultrasound (US) and plasma-activated water (PAW) was developed to better preserve crayfish. First, the decontamination efficacy of US, PAW and their combinations (UP) on crayfish was quantified after 0, 20, 40, or 60 min of treatments. The total viable count (TVC) was reduced by 0.27-0.77 Log CFU/g after individual US or PAW treatments, while a TVC reduction of 1.17 Log CFU/g was achieved after 40 min of UP treatment. Besides, the changes in psychrotrophic bacteria, lactic acid bacteria, yeasts and molds followed a similar trend to TVC. UP treatments normally resulted in more significant reductions in the natural microbiota of crayfish than US or PAW treatments. Furthermore, the microbial quality, physicochemical properties and sensory properties of crayfish after different treatments were assessed during storage at 4 °C for 12 days. According to TVC and total volatile basic nitrogen (TVB-N) values, the control group became unacceptable from 4 days, US or PAW groups became unacceptable from 6 days, while UP group extended the storage time to 8-10 days. During storage, thiobarbituric acid reactive substances (TBARS) values of all the groups were maintained below 0.5 mg/kg, among which the control group exhibited the highest value (0.39 mg/kg). Moreover, UP treatment effectively retarded the deterioration in color and texture properties of crayfish. Fourier transform infrared (FTIR) spectroscopy analysis indicated that UP treatment decreased the α-helix contents and increased the ß-sheet contents of crayfish proteins, while the structural changes were not evident at the end of storage. Low-field nuclear magnetic resonance (LF-NMR) analysis revealed that UP treatment reduced the water migration and enhanced the stability of bond water in crayfish. In addition, E-nose analysis revealed the protection of UP treatment on the sensory properties of crayfish during storage. This study demonstrated that the combinations of US and PAW treatments effectively accelerated the decontamination of crayfish and contributed to better storage quality.

High-Performance, Degradable, Self-Healing Bio-Based Nanocomposite Coatings with Antibacterial and Antioxidant Properties.

The purpose of this study is to obtain a bio-based coating with good functional activity and self-healing ability, demonstrating its potential in food, materials, and other application fields. Plastic coatings can cause serious environmental pollution. It was a good solution to replace plastic coatings with degradable coatings. However, the development of degradable coatings in the fields of food and materials was limited due to their insufficient antibacterial ability and weak comprehensive properties. Therefore, chitosan nanoparticles (NPs) loaded with gallic acid (GA) were self-assembled with gelatin (GE) to prepare high-performance, degradable, self-healing bio-based nanocomposite coatings with antibacterial and antioxidant properties. The oxygen permeability of GE nanocomposite coatings decreased gradually with the addition of NPs, and the barrier properties increased significantly. At the same time, due to the excellent antioxidant and antibacterial ability of GA, the antioxidant effect of the nanocomposite coatings increased by 119%, and the antibacterial rate against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) increased by 32% and 58%, respectively, compared with the pure GE coatings. In addition, the nanocomposite coatings can be repaired within 24 h after being scratched at room temperature. Finally, GA coated with chitosan nanoparticles can significantly delay the escape of GA, and the retardation of gallic acid release exceeded 89% in simulated solutions after 24 h immersion, extending the service life of the nanocomposite coatings.

Extension of growth/no growth predictive models for the preservation of low-acid pasteurized sauces by incorporating water activity and model validation in sauces.

Combined preservation methods have awakened a growing interest in low-acid pasteurized sauces under ambient storage, aiming to produce more 'natural' foods with enhanced microbial stability. However, limited information and predictive models are available to assess the microbial stability of this kind of products, for which the spoilage is mainly caused by acid-tolerant spore-forming spoilage bacteria (ATSSB). In this study, a set of growth/no growth (G/NG) models developed previously (Sun et al., 2021a) for spores of two ATSSB strains (Bacillus velezensis and Bacillus subtilis) from pasteurized sauces were upgraded to incorporate the effect of water activity (aw). The growth from heat-treated spores (80 °C, 10 min) was assessed during three months in Nutrient Broth under 320 combinations of storage temperatures (22 and 30 °C), aw (0.95 and 0.965), pH (4.4-5.6), total acetic acid (0.0-0.3 % (w/w)), and total lactic acid (0.00-1.00 % (w/w)). Twelve replicates were tested for each combination. After merging the dataset collected previously at aw 0.98, a set of 4-variate (4V) models were developed for each strain under a single temperature after 30, 60 and 90 days separately. Additionally, the developed models were validated by challenge tests in different industrially produced low-acid pasteurized sauces for three months. The results showed that lowering aw significantly enhanced the antimicrobial effect of decreased pH and the addition of acetic and lactic acid against the ATSSB spores, while the enhancement was less evident at high acetic acid concentrations. In the challenge tests, the developed models exhibited accurate predictions for the sauces, with the % correct-predicted values ≥90 %. The developed models can be used in microbiological risk assessments or the innovation of preservation strategies for low-acid pasteurized sauces.

Modeling the combined effect of temperature, pH, acetic and lactic acid concentrations on the growth/no growth interface of acid-tolerant Bacillus spores.

The application of minimal processing technologies has led to increased spoilage incidents in low-acid pasteurized sauces due to the outgrowth of acid-tolerant spore-forming spoilage bacteria (ATSSB). Controlling the germination and subsequent growth of ATSSB spores is vital to enhance the ambient storage stability of pasteurized sauces. This study developed and validated a set of growth/no growth (G/NG) models for spores of two ATSSB strains (Bacillus velezensis and Bacillus subtilis subsp. subtilis) isolated from pasteurized sauces. The G/NG data at two levels of temperature (22 and 30 °C) were collected in Nutrient Broth (aw = 0.98 adjusted with NaCl) by a full factorial design with five equidistant levels of pH (4.4-5.6), four concentrations of total acetic acid (0.0-0.3% (w/w)), and four concentrations of total lactic acid (0.00-1.00% (w/w)). The growth, starting from heat-treated (10 min 80 °C) spores, of each strain was assessed under 160 combinations by regular optical density measurements during three months. Twelve replicates were made for each combination. The developed models demonstrate that without organic acids even the lowest pH (4.4) allows a high growth possibility of the ATSSB spores, while acetic and lactic acids exhibit a significant antibacterial activity, which can be enhanced at decreased pH. The growth starting from B. subtilis spores can be inhibited for at least three months with 1.0% (w/w) total lactic acid in the water phase at both temperatures, which was not the case for B. velezensis, while 0.3% acetic acid achieves a full inhibition on both strains at 22 °C. With a combination of 0.3% acetic acid and 0.7% lactic acid, no growth should occur in the investigated range. This research is one of the first studies exploring the feasibility of ambient storage for low-acid pasteurized sauces eliminating preservatives such as benzoic and sorbic acids, and proves the synergistic effect of decreased pH and the presence of acetic and lactic acids on inhibiting bacterial growth from ATSSB spores.