[Study of Peak Carbon Emission of a City in Yangtze River Delta Based on LEAP Model].

Based on the LEAP model framework, a LEAP-X sub-sector calculation model suitable for X City was constructed in this study. Four scenarios including a baseline scenario, low-carbon scenario, enhanced low-carbon scenario, and peak in 2023 scenario were set up to predict and analyze the carbon emission situation. The calculation and analysis results showed that it could achieve the carbon peak before 2030 only under the enhanced low-carbon scenario and peak in 2023 scenario. The peak year of the enhanced low-carbon scenario was around 2025 with a peak carbon emission of approximately 170 million tons, but the peak time may actually be delayed. Industry was the largest sector of carbon emissions, and the petrochemical industry was the largest portion of industry, the proportion of which was always maintained at approximately 30% under different scenarios. However, the proportion of power generation and steel industry decreased annually, whereas the proportion of the net imported power gradually increased. Industrial structure optimization and energy structure adjustment were the main driving factors of carbon peak in X City. Carbon emissions per unit of GDP will fall by approximately 41% in 2030 compared with that in 2020 under the enhanced low-carbon scenario.

Newly Detected Transmission of blaKPC-2 by Outer Membrane Vesicles in Klebsiella Pneumoniae.

OBJECTIVE: The prevalence of carbapenem-resistant Klebsiella pneumoniae (CR-KP) is a global public health problem. It is mainly caused by the plasmid-carried carbapenemase gene. Outer membrane vesicles (OMVs) contain toxins and other factors involved in various biological processes, including ß-lactamase and antibiotic-resistance genes. This study aimed to reveal the transmission mechanism of OMV-mediated drug resistance of Klebsiella (K.) pneumoniae. METHODS: We selected CR-KP producing K. pneumoniae carbapenemase-2 (KPC-2) to study whether they can transfer resistance genes through OMVs. The OMVs of CR-KP were obtained by ultracentrifugation, and incubated with carbapenem-sensitive K. pneumoniae for 4 h. Finally, the carbapenem-sensitive K. pneumoniae was tested for the presence of blaKPC-2 resistance gene and its sensitivity to carbapenem antibiotics. RESULTS: The existence of OMVs was observed by the electron microscopy. The extracted OMVs had blaKPC-2 resistance gene. After incubation with OMVs, blaKPC-2 resistance gene was detected in sensitive K. pneumoniae, and it became resistant to imipenem and meropenem. CONCLUSION: This study demonstrated that OMVs isolated from KPC-2-producing CR-KP could deliver blaKPC-2 to sensitive K. pneumoniae, allowing the bacteria to produce carbapenemase, which may provide a novel target for innovative therapies in combination with conventional antibiotics for treating carbapenem-resistant Enterobacteriaceae.

[Soil Properties, Heavy Metal Accumulation, and Ecological Risk in Vegetable Greenhouses of Different Planting Years].

The vegetable greenhouse soils in Yanglou Town, Ruzhou City, Henan Province were taken as the research object in the present study to explore the difference in soil physical and chemical properties and the total and fraction of heavy metals of different planting years. The potential ecological risks of heavy metals in greenhouse soils with different planting years were assessed by using single and comprehensive potential ecological risk index methods. The results showed that the soil pH of vegetable greenhouses increased, and fertility factors such as organic matter, available phosphorus, and alkali-hydrolyzable nitrogen accumulated to a certain extent compared to the control group, whereas catalase showed a decreasing trend. Correlation analysis showed that the planting years were significant positively correlated with pH (P<0.05) and organic matter (P<0.01) and significant negatively correlated with catalase (P<0.01). The amount of heavy metals in the vegetable greenhouse soils increased with the increase in planting years, among which Cu, Zn, and Cd increased most obviously, with maximum increases of 129.14%, 204.17%, and 161.11%, respectively. The proportion of acid-soluble and reducible heavy metals in the vegetable greenhouse soils also increased gradually with the planting years, and the proportion of residual heavy metals decreased correspondingly, which resulted in the heavy metals transforming into fractions easily absorbed by plants. The results of the single potential ecological risk index showed that Cd in vegetable greenhouse soils had a strong ecological risk with the increase in planting years, whereas Cu, Pb, Zn, and Ni were in the mild risk category. The comprehensive potential ecological risk index showed that the heavy metals in the vegetable greenhouse soils of different planting years have reached a strong or very strong ecological risk.