Efficacy of Seamless Care Management Under FOCUS-PDCA for Patients with Acute Cerebral Infarction Complicated with Dysphagia and its Influence on Nutritional Status and Neurological Functions.

Objective: To evaluate the efficacy of seamless care management under FOCUS-PDCA for patients with acute cerebral infarction (ACI) complicated with dysphagia and its influence on nutrition status and neurological functions. Methods: A total of 98 patients with acute cerebral infarction complicated with dysphagia admitted to our hospital from March 2019 to December 2022 were selected and divided into two groups with equal numbers. The control group received routine nursing management. The study group implemented seamless nursing management under FOCUS-PDCA based on the control group. The therapeutic effect, nutritional status, and neurological function of the two groups were compared before and after intervention. Patients in both groups were intervened for 1 month. Results: (1) After the intervention, the Water Drinking Test (WDT) Grade I and Grade II of the study group increased from 0.00% before the intervention to 32.65%, and the control group also increased from 0.00% before the intervention to 14.29% and 22.45%. , while the number of Grade III, Grade IV, and Grade V decreased in both the control group and the study group. That is, the WDT level test of both groups after the intervention was better than before the intervention, and the research group was better than the control group, and the difference was statistically significant (Z=2.732, P = .006). (2) There was no statistical significance in the GUSS score and SWAL-QOL of the two groups before the intervention (P > .05); after the intervention, the GUSS score of the control group (16.32±4.26) was lower than that of the study group (18.52±4.85) (P < .001) , the SWAL-QOL score of the control group (116.48±31.05) was lower than that of the study group (143.08±33.85) (P < .001), and the GUSS scores and SWAL-QOL of the two groups after the intervention were higher than before the intervention (P < .001). (3) Compared with the nutritional status of the two groups, there was no statistical significance in PA, ALB, and Hb before intervention (P > .05); after intervention, PA, ALB, and Hb in the control group were (218.15±27.15) and (38.67±5.84) respectively. (120.85±31.05), all lower than the study group (261.15±22.52), (42.84±6.32), (134.18±30.47) (P < .01), and the PA, ALB and Hb of the two groups after intervention were all higher than before intervention (P < .001). (4) Comparing TSF and MAMC between the two groups, there was no statistical significance in TSF and MAMC before intervention (P > .05); after intervention, TSF and MAMC in the control group were (13.84±6.38) and (28.61±4.15) respectively, both of which were low. Compared with the study group (16.94±6.69), (33.15±3.84) (P < .001), and the TSF and MAMC of the two groups after the intervention were higher than before the intervention (P < .001). (5) Comparing the neurological function and limb function between the two groups, there was no statistical significance in NIHSS and FMA before intervention (P > .05); after intervention, NIHSS and FMA in the control group were (19.64±3.52) and (48.15±4.85) respectively. The NIHSS was higher than that of the study group (16.52±3.85), and the FMA was lower than that of the study group (58.84±5.63) (P < .001). The NIHSS and FMA of both groups after the intervention were better than those before the intervention (P < .001). (6) The incidence rate of complications in the study group was 4.08%, which was lower than 18.37% in the control group (P < .05). Conclusion: Seamless nursing management for patients with ACI and dysphagia under the FOCUS-PDCA model has obvious effects. It can effectively improve the patient's swallowing function, reduce the degree of neurological deficit, improve their nutritional status and quality of life, and has fewer complications. It can be used clinically Promote within the application.

MicroRNA-143 suppresses oral squamous cell carcinoma cell growth, invasion and glucose metabolism through targeting hexokinase 2.

miRNAs are non-coding RNAs that have functions to regulate gene expression and play essential roles in a variety of biological processes of cancers. In the present study, we report miR-143 acts as a tumor suppressor in human oral squamous cell carcinoma (OSCC). The expressions of miR-143 are down-regulated in both OSCC cell lines and patient samples compared with normal adjacent tissues. We found overexpression of miR-143 in oral cancer cell lines suppresses cell migration, cellular glucose metabolism and proliferation. Moreover, overexpression of miR-143 promoted apoptosis and significantly caused cell cycle arrest at G1 stage. The colony formation of oral cancer cells was also suppressed by miR-143 We identified hexokinase 2 (HK2) as a direct target of miR-143 in oral cancer cells. Our data show that miR-143 complementary pairs to the 3'-UTR of HK2 in oral cancer cells, leading to the inhibition of glycolysis in vitro and in vivo Moreover, knockdown of HK2 by siRNA in oral cancer cells inhibited glucose metabolism, proliferation and migration. Recovery of glucose metabolism by overexpression of HK2 in miR-143 overexpressing cells restores the cell migration and proliferation, suggesting that the miR-143-mediated cancer suppression is through the direct inhibition of HK2. In summary, the present studies highlight miR-143 as a tumor suppressor in OSCC by the suppression of cell migration, glucose metabolism and proliferation through directly targeting HK2, rendering miR-143 a therapeutic strategy for the treatment of clinical OSCC patients.

Polychlorinated biphenyls in alfalfa: Accumulation, sorption and speciation in different plant parts.

The accumulation, chemical speciation and distribution of polychlorinated biphenyls (PCBs) were investigated in various parts of alfalfa. Moreover, the adsorption characteristics for PCB 28 by alfalfa and the influencing factors of the adsorption characteristics were studied. There were different degrees of PCB accumulation in alfalfa roots, root nodules and shoots. The decreasing order of the accumulation of PCBs in plant tissues was root nodules > roots > shoots, and the decreasing order of the total PCB contents was roots > shoots > root nodules, indicating that the roots were the main sink for PCB accumulation. There were three modes of PCB speciation in alfalfa roots and root nodules, comprising strong sorption (78%) and weak sorption (19%) on tissue surfaces and absorption within tissues (2%). The adsorption isotherms of PCB 28 indicate that the adsorption capacities of root nodules and shoots were both significantly higher than that of the roots. Both lipids and carbohydrates, and especially lipids, affected the PCB adsorption capacities of the tissues. These results may help in the elucidation of the mechanisms of sorption and accumulation of PCBs in the plants and their main influencing factors and thus contribute to the development of phytoremediation technologies for PCB-contaminated soils.

Phytoremediation of soil contaminated with cadmium, copper and polychlorinated biphenyls.

A pot experiment and afield trial were conducted to study the remediation of an aged field soil contaminated with cadmium, copper and polychlorinated biphenyls (PCBs) (7.67 +/- 0.51 mg kg(-1) Cd, 369 +/- 1 mg kg(-1) Cu in pot experiment; 8.46 +/- 0.31 mg kg(-1) Cd, 468 +/- 7 mg kg(-1) Cu, 323 +/- 12 microg kg(-1) PCBs for field experiment) under different cropping patterns. In the pot experiment Sedum plumbizincicola showed pronounced Cd phytoextraction. After two periods (14 months) of cropping the Cd removal rates in these two treatments were 52.2 +/- 12.0 and 56.1 +/- 9.1%, respectively. Total soil PCBs in unplanted control pots decreased from 323 +/- 11 to 49.3 +/- 6.6 microg kg(-1), but with no significant difference between treatments. The field microcosm experiment intercropping of three plant species reduced the yield of S. plumbizincicola, with a consequent decrease in soil Cd removal. S. plumbizincicola intercropped with E. splendens had the highest shoot Cd uptake (18.5 +/- 1.8 mg pot(-1)) after 6 months planting followed by intercropping with M. sativa (15.9 +/- 1.9 mg pot(-1)). Liming with S. plumbizincicola intercropped with M. sativa significantly promoted soil PCB degradation by 25.2%. Thus, adjustment of soil pH to 5.56 combined with intercropping with S. plumbizincicola and M. sativagave high removal rates of Cd, Cu, and PCBs.

Potential for biodegradation of polychlorinated biphenyls (PCBs) by Sinorhizobium meliloti.

Resting cell assay and soil microcosms were set up to investigate the biodegradation capability and metabolic intermediate of polychlorinated biphenyls (PCBs) by a rhizobial strain Sinorhizobium meliloti. Biodegradation was observed immediately after 2,4,4'-TCB was supplied as a sole source of carbon and energy in liquid cultures. After 6 days, the percent biodegradation of 2,4,4'-TCB was 77.4% compared with the control. The main intermediate was identified as 2-hydroxy-6-oxo-6-phenylhex-2,4-dienoic acid (HOPDA) for 2,4,4'-TCB as determined by gas chromatography-mass spectrometry (GC-MS). Inoculation with S. meliloti greatly enhanced the degradation of target PCB mixtures in the soil. Moreover, soil culturable bacteria, fungi and biphenyl degrading bacteria counts showed significant increase after inoculation of S. meliloti. This study suggests that S. meliloti is promising in PCB bioremediation.

Influence of Rhizobium meliloti on phytoremediation of polycyclic aromatic hydrocarbons by alfalfa in an aged contaminated soil.

Microbe-assisted phytoremediation is emerging as one of the most effective means by which plants and their associated rhizosphere microbes degrade organic contaminants in soils. A pot study was conducted to examine the effects of inoculation with Rhizobium meliloti on phytoremediation by alfalfa grown for 90 days in an agricultural soil contaminated with weathered polycyclic aromatic hydrocarbons (PAHs). Planting with uninoculated alfalfa (P) and alfalfa inoculated with R. meliloti (PR) significantly lowered the initial soil PAH concentrations by 37.2 and 51.4% respectively compared with unplanted control soil. Inoculation with R. meliloti significantly increased the counts of culturable PAH-degrading bacteria, soil microbial activity and the carbon utilization ability of the soil microbial community. The results suggest that the symbiotic association between alfalfa and Rhizobium can stimulate the rhizosphere microflora to degrade PAHs and its application may be a promising bioremediation strategy for aged PAH-contaminated soils.

Influence of arbuscular mycorrhiza and Rhizobium on phytoremediation by alfalfa of an agricultural soil contaminated with weathered PCBs: a field study.

A field experiment was conducted to study the effects of inoculation with the arbuscular mycorrhizal fungus Glomus caledonium and/or Rhizobium meliloti on phytoremediation of an agricultural soil contaminated with weathered PCBs by alfalfa grown for 180 days. Planting alfalfa (P), alfalfa inoculated with G. caledonium (P + AM), alfalfa inoculated with R. meliloti (P + R), and alfalfa co-inoculated with R. meliloti and G. caledonium (P+AM+R) decreased significantly initial soil PCB concentrations by 8.1, 12.0, 33.8, and 43.5%, respectively. Inoculation with R. meliloti and/or G. caledonium (P+AM+R) increased the yield of alfalfa, and the accumulation of PCBs in the shoots. Soil microbial counts and the carbon utilization ability of the soil microbial community increased when alfalfa was inoculated with R. meliloti and/or G. caledonium. Results of this field study suggest that synergistic interactions between AMF and Rhizobium may have great potential to enhance phytoremediation by alfalfa of an agricultural soil contaminated with weathered PCBs.

[Sorption behavior of PCB28 to phosphatidylcholine and triglyceride].

Sorption behaviors of PCB28 to phosphatidylcholine (PC)and triglyceride (TG) were studied. Results showed that sorption equilibrium could be achieved in 8 h for PCB28, and the sorption amount on PC was higher than that of TG when the initial PCB28 concentration is in the range of 50-250 microg x L(-1). As the initial PCB28 concentration increases, the absolute sorption amount and relative sorption amount on PC and TG enhances. And linear isotherm is suggested as the most appropriated fitting type, sorption constants K(d) calculated from linear isotherm for PC and TG are 38.84 L x g(-1) and 20.14 L x g(-1) respectively, which suggested that PC had a higher sorption ability to PCB28 than that of TG, and the fatty acid chain length might be important factors to influence the sorption mechanism of PC and TG.

[Phytoremediation of polychlorinated biphenyls contaminated soil by leguminosae-gramineae intercropping: a field trial].

Phytoremediation of polychlorinated biphenyls (PCBs) contaminated soil by leguminosae (alfalfa) and gramineae (ryegrass and tall fescue) was studied in a field trial. All planted treatments had significantly higher PCBs removal efficiency compared with those of unplanted control after 270 days of in-situ phytoremediation. Alfalfa monoculture received the highest removal efficiency of soil PCBs by 59.6%. Analysis of PCBs congeners composition showed that the percentage of di-chlorinated biphenyl decreased in all planted treatments. Alfalfa produced the maximum biomass among the 3 plants. Total PCBs concentration in alfalfa roots reached 355.1 microg/kg, which was significantly higher than those in ryegrass and tall fescue. The phytoextraction efficiency of different treatment was in order of alfalfa > alfalfa-ryegrass-tall fescue > alfalfa-ryegrass > ryegrass > alfalfa-tall fescue > tall fescue. The results suggest that alfalfa may be an ideal candidate for the phytoremediation of PCBs contaminated soil.

A new stromata-producing Neotyphodium species symbiotic with clonal grass Calamagrostis epigeios (L.) Roth. grown in China.

We describe a new stromata-producing Neotyphodium species symbiotic with clonal Calamagrostis epigeios (L.) Roth. Stromata on the grass, 47.5-186 mm long, occurred frequently, but neither perithecium nor mature ascus was observed. Morphology of fungal isolates obtained from symptomatic and asymptomatic tillers were identical to each other and similar to those of epichloë endophytes. In phylogenetic analysis all selected five fungal isolates clustered into a significantly distinct clade based on sequences of beta-tubulin gene (tubB) introns and translation elongation factor 1-alpha gene (tefA) introns with bootstrap values of 99%, supporting erection of a new species. Concerning the production of extremely long stromata on the host plants and absence of sexual spores, we propose the name Neotyphodium stromatolongum Y. Ji, L. Zhan et Z. Wang, sp. nov.