ABSTRACT
A study was carried out on the characteristic of lead absorption in pumpkin via atomic absorption spectrophotometer. The results showed that lead absorption amount in pumpkin increased with time, but the absorption rate decreased with time; And the lead absorption amount reached the peak in pH 7. Lead and cadmium have similar characteristic of absorption in pumpkin.
Subject(s)
Cucurbita/metabolism , Lead/pharmacokinetics , Spectrophotometry, Atomic/methods , Absorption , Hydrogen-Ion Concentration , Time FactorsABSTRACT
Basing on the fragment (CO434610) cloned from cDNA amplified fragment length polymorphism (cDNA-AFLP), a 1191-bp cDNA sequence of CsEXP10 with a complete 3' end was obtained via rapid amplification of cDNA ends (RACE) and getting spliced with an EST (AF319471) of the same gene (Fig.2). Southern blotting analysis showed that CsEXP10 was a single copy gene in cucumber (Fig.5). The transcripts of CsEXP10 were detected by RT-PCR only in young fruit 3 d after pollination, but not in root, stem, and leaf (Fig.4A). At different developmental stages of fruit growth, Northern blotting demonstrated that CsEXP10 gene was expressed strongly in fruit 3 d and 10 d after pollination, especially the latter, but undetectable in ovaries of 2-3 cm in length and without pollination on the day flowering, and in fruits 26 d after pollination (Fig.4B). It is suggested that CsEXP10 might be highly correlated with the expansive growth of cucumber fruit.
Subject(s)
Cucumis sativus/genetics , Fruit/genetics , Plant Proteins/genetics , Amino Acid Sequence , Base Sequence , Blotting, Northern , Blotting, Southern , Cloning, Molecular , Cucumis sativus/growth & development , DNA, Complementary/chemistry , DNA, Complementary/genetics , Fruit/growth & development , Gene Expression Regulation, Developmental , Gene Expression Regulation, Plant , Molecular Sequence Data , Reverse Transcriptase Polymerase Chain Reaction , Sequence Analysis, DNA , Sequence Homology, Amino AcidABSTRACT
Differential expression of genes in young fruit of cucumber (Cucumis sativus L.) before/after pollination was investigated via cDNA amplified fragment length polymorphism (cDNA-AFLP). There were 64 transcript-derived fragments (TDFs) displayed specially in young fruits after pollination of 6-72 h (Table 1), five of which were verified to be mainly expressed in young fruits by reverse Northern dot blotting (Fig.2). Sequence data showed one TDF was a new gene encoding expansin and was named as Cs-Expansin10 (CsExp10), which might be related to the expanding growth of cucumber fruit after pollination (Fig.3), another was significantly similar to the glutathione reductase gene, and the others had no similar sequence in GenBank database (Table 2).
Subject(s)
Cucumis sativus/genetics , Gene Expression Profiling , Pollination/genetics , Amino Acid Sequence , Amplified Fragment Length Polymorphism Analysis , Base Sequence , Blotting, Northern , Cucumis sativus/growth & development , Cucumis sativus/metabolism , Gene Expression Regulation, Developmental , Gene Expression Regulation, Plant , Molecular Sequence Data , Plant Proteins/genetics , Pollination/physiology , Sequence AlignmentABSTRACT
OBJECTIVE: To observe the clinical efficacy of Qingqiao Capsule (QQC) in treating patients with secretory otitis media (SOM). METHODS: A total of 90 patients were randomly assigned into the treated group (n = 45) and the control group (n = 45). Patients in the treated group were administrated with QQC, 5 capsules each time, 3 times a day for totally 10-14 days, and those in the control group were given per os cefaclor capsules 0.5 g each time for adult, 3 times a day, or 20 mg/(kg.d) for children, for 10-14 days. The therapeutic efficacy of treatment on the patients was observed and compared after treatment and followed up for 3-6 months. RESULTS: (1) The clinical efficacy in the treated group was superior to that in the control group with significant statistical difference (P < 0.01); (2) Comparison of the efficacies in patients of three different TCM syndrome types (the external pathogenic wind invasion caused auditory orifice stuffiness type, the Gan-Dan damp-heat steaming up auditory orifice type and the Pi-deficiency dysfunction induced dirty dampness blocking ear type) showed no statistically significant difference (P > 0.05); (3) The vanishing rate and time needed of the main symptoms and signs in the treated group were superior to those in the control group on ear muffle, tinnitus, hearing impairment, hydrotypanum, pure tone threshold and abnormal tongue figure, and the difference was statistically significant (P < 0.05 or P < 0.01), only those of earache, otopiesis and abnormal pulse figure were insignificantly different between the two groups (P > 0.05). CONCLUSION: QQC is an effective Chinese composite medicine on patients with SOM, and shows no obvious adverse reaction.
