Study of in vivo brain glioma in a mouse model using continuous-wave terahertz reflection imaging.

We demonstrated that in vivo brain glioma in a mouse model using a continuous-wave terahertz reflection imaging system, as well as the ex vivo fresh brain tissues in mouse model. The tumor regions of in vivo and ex vivo brain tissues can be well distinguished by THz intensity imaging at the frequency of 2.52THz. The THz images with high sensitivity correlated well with magnetic resonance, visual and hematoxylin and eosin stained images. Furthermore, the THz spectral difference between brain gliomas and normal brain tissues were obtained in the 0.6THz to 2.8THz range, where brain gliomas have the higher refractive indices and absorption coefficients, and their differences increase particularly in the high frequency range. These results suggest that THz imaging has great potential as an alternative method for the intraoperative label-free diagnosis of brain glioma in vivo.

Croceitalea marina sp. nov., isolated from marine particles of Yellow Sea, and emended description of the genera Croceitalea.

A Gram-staining-negative, rod-shaped, non-motile, golden yellow-coloured and strictly aerobic bacterial strain, designated H01-35T, was isolated from a surface marine particles sample collected from the Yellow Sea in China. According to the phylogenetic analysis based on 16S rRNA gene sequences, the strain H01-35T belonged to the genus Croceitalea and showed the highest sequence similarity to Croceitalea litorea CBA3205T (96.4 %). Strain H01-35T grew optimally at pH 8.0-9.0, 28 °C and in the presence of 3 % (w/v) NaCl. The DNA G+C content was 52.7 mol%. Strain H01-35T contained MK-6 as the predominant respiratory quinone and held iso-C15 : 1 G, iso-C15 : 0 and iso-C17 : 0 3-OH as the major cellular fatty acids. The major polar lipids were phosphatidylethanolamine, two unidentified aminolipids and five unidentified lipids. Exoenzymes for starch, gelatin and Tween 20 degradation were detected in Strain H01-35T but the strain was negative for sulfur and indole production. On the basis of the polyphasic analyses, this isolate was considered to represent a novel species in the genus Croceitalea, for which the name Croceitalea marina sp. nov. is proposed. The type strain is H01-35T (MCCC 1K03229T=KCTC 52368T). The emendation of description of the genus Croceitalea is also given.

Roseibium sediminis sp. nov., isolated from sea surface sediment.

A Gram-stain-negative, rod-shaped, facultatively aerobic strain, motile by a monotrichous (polar or lateral) flagellum, designated BSS09T, was isolated from surface sediment of the Bohai Sea, PR China. Growth was observed at 10-45 °C (optimum 32 °C), in the presence of 1.0-7.0 % (w/v) NaCl (optimum 4.0 %) and at pH 5.0-8.0 (optimum pH 6.0). Phylogenetic analysis, based on 16S rRNA gene sequences, revealed that strain BSS09T belonged to the genus Roseibium and showed the highest sequence similarity of 96.5 % to Roseibium hamelinense JCM 10544T. The significant dominant fatty acid was C18 : 1ω7c. The polar lipids comprised one phosphatidylcholine, one phosphatidylmonomethylethanolamine, one unidentified aminolipid, one phosphatidylglycerol, one phosphatidylethanolamine and one unidentified polar lipid. The major respiratory quinone was Q-10. The DNA G+C content of strain BSS09T was 57.1 mol%. On the basis of evidence from this polyphasic study, strain BSS09T is proposed to represent a novel species of the genus Roseibium, for which the name Roseibium sediminis sp. nov. is proposed. The type strain is BSS09T (=KCTC 52373T=MCCC 1K03201T).

Polaribacter pacificus sp. nov., isolated from a deep-sea polymetallic nodule from the Eastern Pacific Ocean.

A Gram-staining-negative, yellow-colony-forming, rod-shaped, non-flagellated and facultatively aerobic strain, designed HRA130-1T, was isolated from a deep-sea polymetallic nodule from the Pacific Clarion-Clipperton Fracture Zone (CCFZ). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain HRA130-1T belonged to the genus Polaribacter (96.3-93.2 % 16S rRNA gene sequence similarity), and exhibited 94 % 16S rRNA gene sequence similarity to Polaribacter filamentus KCTC 23135T (type species) and the highest sequence similarity to Polaribacter huanghezhanensis KCTC 32516T (96.3 %). Optimal growth occurred in the presence of 4 % (w/v) NaCl, at pH 7.0 and 16 °C. The DNA G+C content of strain HRA130-1T was 35.9 mol%. The major fatty acid was iso-C15 : 0. The predominant respiratory quinone was menaquinone-6 (MK-6). The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, one unidentified phospholipid and an unidentified aminolipid. On the basis of data from the present taxonomic study using a polyphasic approach, strain HRA130-1T represents a novel species of the genus Polaribacter, for which the name Polaribacter pacificus sp. nov. is proposed. The type strain is HRA130-1T (=KCTC 52370T=MCCC 1K03199T=JCM 31460T=CGMCC 1.15763T).

The expression of hTERT mRNA and cellular immunity in gastric cancer and precancerosis.

AIM: To observe the expression of Human telomerase reverse transcriptase (hTERT) in gastric carcinomas and precancerosis lesions, to evaluate the immune state of such patients, and to then study the clinical significance of hTERT and immune state for the diagnosis, treatment and prognosis of gastric cancer. METHODS: In situ hybridization was used to detect the expression of hTERT mRNA in 116 endoscopic of gastric mucosa. Analyzed tissue samples were as follows: 30 cases of chronic superficial gastritis (CSG), 44 of precancerosis lesions (including 27 of chronic atrophic gastritis, 8 of adenomatous polyp and 9 of gastric ulcer) and 42 of gastric cancer (GC). In addition, the T lymphocyte subsets (CD3(+), CD4(+), CD8(+), CD4(+)/CD8(+)) and natural killer cells (NK) in peripheral blood were determined by flow cytometric analysis (FCM) in 30 cases of CSG, 27 of precancerosis (chronic atrophic gastritis, CAG), and 42 of GC. The data were compared with those of normal control (NC). RESULTS: The detected positive rate of hTERT varied as follows: 86 % (36/42) in GC, 36 % (16/44) in precancerosis lesions and 0 % (0/30) in CSG. The expression of hTERT mRNA was not associated with patient gender, tumor location, macroscopic type, lymph node metastasis, or degree of differentiation. It was found that the CD3(+), CD4(+) of the CSG group were lower than that of NC (P<0.05). Meanwhile, the T lymphocyte subsets (CD3(+), CD4(+), CD4(+)/CD8(+) ratio) and NK cells of CAG were remarkably lower than that of NC and CSG groups (P<0.05-0.01). Values of T cells and NK cells of the GC group were significantly abnormal when compared with the CAG group (P<0.05-0.01). Furthermore, with tumor progression, the function of T cells was weakened gradually. CONCLUSION: The expression of telomerase may be a crucial step in gastric carcinogenesis and increased hTERT mRNA may serve as a novel marker for diagnosis of GC. The immune state of patients with GC and precancerosis was somewhat depressed, which indicates the importance of cellular immunological assays in cancer patients.