Numerical study of the upwelling and downwelling effects of artificial reefs along tidal cycles in the Pearl River Estuary.

Artificial reefs (ARs) are a preferred option for managers due to their distinctive hydrodynamic properties, which support a highly productive local ecosystem. However, the hydrodynamics characteristics of ARs in natural marine environments have not been conducted. Being the first to explore the spatiotemporal characteristic of flow fields around ARs along tidal cycles in marine environments, this study redefined the upwelling and downwelling of ARs, based on natural vertical velocities, and separated the upwelling into co-direction upwelling and re-direction upwelling, and the downwelling into co-direction downwelling and re-direction downwelling. This study simulated the flow field in the Wanshan ARs area of the Pearl River Estuary along the tidal cycles using the MIKE3-FM. Numerical simulations revealed that (1) co-direction upwelling and co-direction downwelling were the dominant components of the vertical flow field effects of ARs; (2) the areas sum of upwelling and downwelling were largest in the medium water column, with about 1.6 and 1.03 times as large as the bottom and surface water column, respectively, while the fluxes sum of the upwelling and downwelling were largest in bottom water column, with approximately 1.3 and 2.2 times larger than those in the middle and surface water columns; (3) the area and volume of the upwelling and downwelling gradually decreased along neap-spring tide, exhibited significantly negative correlations with current speeds; while the upwelling flux and downwelling flux gradually increased along neap-spring tide; exhibited a significantly positive correlation with current speed; (4) the effects of tide to upwelling and downwelling of AR are forced by the northward velocity of current speed, the net flux of upwelling and downwelling showed a significant positive correlation with the northward velocity of current speed (r = 0.94). These results could provide a reference for assessing the flow field effect of ARs and a guide for the configuration and management of ARs.

Inoculation with plant growth-promoting rhizobacteria improves seagrass Thalassia hemprichii photosynthesis performance and shifts rhizosphere microbiome.

Plant growth-promoting rhizobacteria (PGPR) inoculation is a crucial strategy for maintaining the sustainability of agriculture and presents a promising solution for seagrass ecological restoration in the face of disturbances. However, the possible roles and functions of PGPRs in the seagrass rhizosphere remain unclear. Here, we isolated rhizosphere bacterial strains from both reef and coastal regions and screened two PGPR isolates regarding their in vivo functional traits. Subsequently, we conducted microcosm experiments to elucidate how PGPR inoculation affected seagrass photosynthesis and shape within each rhizosphere microbiome. Both screened PGPR strains, Raoultella terrigena NXT28 and Bacillus aryabhattai XT37, excelled at expressing a specific subset of plant-beneficial functions and increased the photosynthetic rates of the seagrass host. PGPR inoculation not only decreased the abundance of sulfur-cycling bacteria, it also improved the abundance of putative iron-cycling bacteria in the seagrass rhizosphere. Strain XT37 successfully colonized the seagrass rhizosphere and displayed a leading role in microbial network structure. As a nitrogen-fixing bacteria, NXT28 showed potential to change the microbial nitrogen cycle with denitrification in the rhizosphere and alter dissimilatory and assimilatory nitrate reduction in bulk sediment. These findings have implications for the development of eco-friendly strategies aimed at exploiting microbial communities to confer sulfide tolerance in coastal seagrass ecosystem.

Effects of artificial reef and fishing moratorium on trophic structure of biological community in the Pearl River Estuary marine ranch based on stable isotopes.

Deployment of artificial reefs (ARs) has become popular technique to create new hard-bottom habitats, increase biodiversity and richness for fisheries. We compared the faunal community structure and food web structure associated with before and after fishing moratorium between ARs and non-ARs in Wanshan Island, Pearl River Estuary using stable isotope techniques. Community composition showed higher differences between ARs and non-ARs. The range of δ13C and δ15N of different functional groups can distinguish the pelagic and benthic trophic pathways of the food web in reef-or-not area before and after fishing moratorium. The isotopic niches of entire faunal, as well as individual functional groups, overlapped less between ARs and non-ARs in Wanshan Island, which makes the isotopic functional indices non-equivalent. The total convex hull area (TA) of ARs was larger than that of non-ARs, indicating that nutrient pathways of ARs were more diverse. Overall, however, these results suggest that trophic structure was convergence between ARs and non-ARs, and differences before and after fishing moratorium, possibly due to seasonal differences. Finally, it was shown that the construction of ARs had a weak effect on the restoration of fishery resources in this area, which might be related to lack of further management, or even similar community composition to non-ARs areas.

The effect of acute toxicity from tributyltin on Liza haematocheila liver: Energy metabolic disturbance, oxidative stress, and apoptosis.

Tributyltin (TBT), a highly toxic and persistent organic pollutant, is widely distributed in coastal waters. Liza haematocheila (L. haematocheila) is one of bony fish distributing coincident with TBT, and exposure risk of TBT to this fish is unknown. In this study, L. haematocheila was exposed to TBT of 0, 3.4, 6.8, and 17.2 µg/L for 48 h to explore hepatic response mechanism. Our results showed that Sn content in livers increased after 48 h of exposure. HSI and histological changes indicated that TBT suppressed liver development of L. haematocheila. TBT reduced ATPase activities. The increased RB in blood and the reduced TBC were measured after exposure to TBT. T-AOC and antioxidant enzymes SOD, CAT, and GPx activities were inhibited while MDA content was increased. Liver cells showed apoptosis characteristics after TBT exposure. Furthermore, transcriptome analysis of livers was performed and the results showed energy metabolism-related GO term (such as ATPase complex and ATPase dependent transmembrance transport complex), oxidative stress-related GO term (such as Celllular response to oxidative stress and Antioxidant activity), and apoptosis-related GO term (such as Regulation of cysteine-type endopeptidase activity involved in apoptosic signaling pathway). Moreover, we found six energy metabolism-related differentially expressed genes (DEGs) including three up-regulated DEGs (atnb233, cftr, and prkag2) and three down-regulated DEGs (acss1, abcd2, and smarcb1); five oxidative stress-related DEGs including one up-regulated DEG (mmp9) and four down-regulated DEG (prdx5, hsp90, hsp98, and gstf9); as well as six apoptosis-related DEGs including five up-regulated DEGs (casp8, cyc, apaf1, hccs, and dapk3) and one down-regulated DEG (bcl2l1). Our transcriptome data above further confirmed that acute stress of TBT led energy metabolic disturbance, oxidative stress, and apoptosis in L. haematocheila livers.

Construction and influencing factors of an early warning system for marine ranching ecological security: Experience from China's coastal areas.

Marine ranching has been widely considered as a new mode of marine fishery production. Marine ranching ecological security (MRES) is the basis and premise to ensure the sustainable utilization of marine ranching functions. In this study, an MRES early warning system was constructed based on comprehensive marine ranching ecological security index (CMRESI) and system dynamic model to reveal the main factors affecting the development of marine ranching and explore the changes in MRES under different future development scenarios in China's coastal areas from 2011 to 2035. The results showed that (1) the mean CMRESI of China was only 0.3265 and spatial heterogeneity was significant, showing a general security state; (2) coupling and coordination degree of MRES subsystems was high in Jiangsu, Fujian, Shandong, and Guangdong, and resources was a major constraint on the coordinated development of MRES in the study area (63.6%); (3) Under the ecological priority development scenario, the CMRESI will be the highest in 2035; however, 27% of MRES (in Jiangsu, Fujian, and Hainan) will continue to issue serious early warnings. This study could provide a reference for construction planning, management maintenance, and decision-making of marine ranching.

Toxicity of tributyltin chloride on haarder (Liza haematocheila) after its acute exposure: Bioaccumulation, antioxidant defense, histological, and transcriptional analyses.

Liza haematocheila is exposed to various chemical contaminants from anthropogenic sources, including tributyltin chloride (TBTC). Yet the toxicity mechanism of TBTC on haarder remains unclear. The haarder was exposed to different doses (0, 10%, 20%, and 50% of LC50-96 h) of TBTC. In this study, the results revealed its high bioaccumulation in the livers and significant alteration for development. The activities of antioxidant enzymes superoxide dismutase, catalase, and glutathione peroxidase decreased after 96-h exposure to TBTC, this accompanied by an increased malondialdehyde level. TBTC exposure caused the intense production of reactive oxygen species, a reduction in total blood cell count in serum, and apoptosis-related alterations in livers, indicating that enhanced oxidative stress occurred in the process of TBTC exposure. Histological results revealed angiorrhexis and infiltration of inflammatory cells, vacuolar degeneration of hepatocytes in the livers, and swelling, fusion, and disintegration of gill organs. Interestingly, the obtained transcriptional profiles indicated that high doses of TBTC caused energy disorder, apoptosis, and adipogenesis restriction mediated by cytokines and adipokines in Jak-STAT and adipocytokine signaling pathways. In summary, acute exposure to high doses of TBTC could impair the antioxidant system and pathways related to energy, apoptosis and adipogenesis, eventually posing a serious challenge to the fitness of haarder individuals and its fish populations as marine resources.

Impacts of conventional and biodegradable microplastics on juvenile Lates calcarifer: Bioaccumulation, antioxidant response, microbiome, and proteome alteration.

Discarded plastic bag is a main component of marine debris, posing potential threats to marine biota. This study was conducted to assess the potential effects of microplastics on juvenile Lates calcarifer. Fish were exposed via diet to two microplastic types from conventional polyethylene (PE) and biodegradable (Bio) plastic bags for 21 days. Antioxidative enzymes activity, intestinal microbiome and proteome were determined. PE and Bio microplastics were found to accumulate in gastrointestinal tracts, and no mortality was observed. Microplastics exposure did not induce significant antioxidant response except for the glutathione reductase (GR) modulation. Intestinal microbiome diversity decreased significantly in PE group based on Simpson index. Both types of microplastics induced proteome modulation by down-regulating proteins associated with immune homeostasis. Bio microplastics maintained higher intestinal microbial diversity and induced more proteins alteration than PE microplastics. This study provides toxicological insights into the impacts of conventional and biodegradable microplastics on juvenile L. calcarifer.

Temporal and spatial comparison of food web structure in marine pastures in the Pearl River Estuary: Implications for sustainable fisheries management.

The biological and ecological integrity of marine ecosystems in the Pearl River Estuary (PRE) has been compromised due to overfishing and water pollution. Fishing moratorium and artificial reef construction have been implemented in Wanshan and Miaowan for resource protection and restoration. Therefore, food web structure and trophic pathways of Wanshan, Miaowan, and Wailingding in different temporal and spatial situation will be determined using the Ecopath model, as well as the keystone species affecting these ecosystems, which can provide a basis for fishery management. The results showed that the energy transfer efficiency of IV and V trophic levels (TL) was higher than that of II and III-TL before and after fishing moratorium, and the energy transfer efficiency of artificial reefs II and III-TL was only slightly higher than that of nonartificial reefs in Wanshan. In addition, the mean values of ecosystem property indicators (consumption, respiration flow, total system throughput, and total biomass) after the fishing moratorium were significantly higher than those before the fishing moratorium. The average value of the ecosystem attribute indicators (consumption, respiration flow, total system throughput, and total biomass) of artificial reefs is lower than those of nonartificial reef areas, which may be related to the differences in community composition between artificial reefs and non-artificial reefs. Finally, Nemipterus japonicus and Gastrophysus spadiceus are keystone species that distinguish the Wanshan and Miaowan artificial reefs from other areas. Overall, the fishing moratorium has a positive effect on the short-term restoration of fishery resources, mainly restoring short-life cycle organisms. However, the construction of artificial reefs will be more conducive to the persistence of ecosystem restoration. In addition, reasonable proliferation, release and fishing of N. japonicus and G. spadiceus will be beneficial to the sustainable utilization of fishery resources.

Rhizosphere microbiome dynamics in tropical seagrass under short-term inorganic nitrogen fertilization.

Rhizosphere microbes are crucial to seagrass meadows because they promote plant growth and heath. However, information concerning the response of rhizosphere microorganisms in seagrass sediment in the presence of different nitrogen sources is lacking. Here, by means of high-throughput sequencing, we investigated how addition of inorganic nitrogen affects the rhizosphere microbiome of the tropical seagrass Thalassia hemperichii. A seagrass culture system was set up to conduct a nitrogen addition (ammonium and nitrate) simulation experiment. We found that the relative abundance of Proteobacteria and Bacteroidetes was increased in inorganic nitrogen-enriched samples, whereas that of Acidobacteria decreased under ammonium enrichment, especially after 35 days. High levels of inorganic nitrogen addition caused a significant decrease in the relative abundance of Desulfobacteraceae, Sulfurovaceae, and Spirochaetes, which are primarily involved in sulfur cycling. Additionally, the abundance of microbes in the seagrass rhizosphere reached the highest after the ammonium-enrichment treatment. Among the analyzed seagrass photosynthetic characteristics, seagrass leaves presented the highest light utility in treatments receiving nitrate, followed by the control groups and ammonium-enrichment groups. Moreover, 16S rRNA gene-predicted functional analysis suggested that some functions related to metabolism of amino acids and signal transduction were enriched in samples receiving high ammonium, whereas nitrate addition enriched predicted functions related to diseases. These findings provide new insights into the response of microbial communities to different types of nitrogen additions in seagrass ecosystems.

Analysis of sea use landscape pattern based on GIS: a case study in Huludao, China.

This study aims to analyse sea use landscape patterns on a regional scale based on methods of landscape ecology integrated with sea use spatial characteristics. Several landscape-level analysis indices, such as the dominance index, complex index, intensivity index, diversity index and sea congruency index, were established using Geographic Information System (GIS) and applied in Huludao, China. The results indicated that sea use landscape analysis indices, which were created based on the characteristics of sea use spatial patterns using GIS, are suitable to quantitatively describe the landscape patterns of sea use. They are operable tools for the landscape analysis of sea use. The sea use landscape in Huludao was dominated by fishing use with a landscape dominance index of 0.724. The sea use landscape is a complex mosaic with high diversity and plenty of fishing areas, as shown by the landscape complex index of 27.21 and the landscape diversity index of 1.25. Most sea use patches correspond to the marine functional zonation plan and the sea use congruency index is 0.89 in the fishing zone and 0.92 in the transportation zone.

[Vegetation landscape health assessment in Changshan Archipelago, North Yellow Sea].

Island vegetation is an important component of island ecosystem. Multi-targets of island ecosystem health integrated with landscape ecology theory were employed to construct the index system for island vegetation health assessment in terms of landscape vigor, landscape stressing intensity and landscape stability. The Changshan Archipelago in the North Yellow Sea was chosen as a case to apply the island vegetation health assessment index system. The results showed that the overall vegetation health status in Changshan Archipelago was good and had a big island variation. The vegetation health index for Haiyang Island and Zhangzi Island was above 0.80, belonging to first eco-health level area, whereas that for Dachangshan Island, Xiaochangshan Island and Dawangjia Island ranged from 0.70 to 0.80, which could be categorized as the second eco-health level area. Guanglu Island and Shichen Island could be termed as the third eco-health level area with the vegetation health index below 0.70. The distance of island to mainland, area of island together with industrial structure were the main driving forces for the variation of vegetation landscape heath between different islands.

Influence of wave and current flow on sediment-carrying capacity and sediment flux at the water-sediment interface.

In nearshore waters, spatial and temporal scales of waves, tidal currents, and circulation patterns vary greatly. It is, therefore, difficult to combine these factors' effects when trying to predict sediment transport processes. This paper proposes the concept of significant wave velocity, which combines the effects of waves, tides, and ocean currents using the horizontal kinetic energy superposition principle. Through a comparison of the relationship between shear stress at the water-sediment interface and sediment-carrying capacity, assuming equilibrium sediment flux, a new formula for sediment-carrying capacity, which incorporates the concept of significant wave velocities, is derived. Sediment-carrying capacity is a function of the critical velocity, which increases with water depth and decreases with increasing relative roughness of the sea bed. Finally, data from field observation stations and simulations are used to test the proposed formula. The results show that the new formula is in good agreement with both field and simulation data. This new formula for sediment-carrying capacity can be used to simulate nearshore sediment transport.

[Research on remote sensing inversion biomass method based on the Suaeda salsa's measured spectrum].

Suaeda salsa is one of characteristic vegetation of wetlands in Northern China. By measuring the spectral data and leaf area index (LAI) of the Suaeda salsa in the ShuangTai Estuary of Liaodong Bay by the use of portable spectrometer and vegetation canopy analyzer, collecting the biomass of the Suaeda salsa samples, setting up the spectral reflectance curve of the Suaeda salsa, probing into the relationship between the vegetation index and the leaf area index of the Suaeda salsa, carrying out regression analysis of LAI and biomass and constructing the function equation, some conclusion were drawn: (1) By the end of September the spectral characteristics of Suaeda salsa show that at the red band 630 nm there is a clear reflection of the peak with a reflection rate of 12%-15%; there is a clear "red valley" configuration between 680 and 700 nm, and there is a clear "red edge" reflection rate of 25%-30% about 760 nm. (2) It was found that there is best correlation between vegetation index (SAVI and MSAVI) and LAI compared to other vegetation index in the regression analysis of the LAI and vegetation index. The correlation coefficient R2 is 0.711. By comparison of vegetation index linear regression equations, the correlation coefficient (SAVI and LADI) R2 is 0.696; the value of R2 (LAI and MSAV) is 0.695; the value of R2 (RVI) is 0.664; the value of R2(NDVI) is 0.649 and the value of R2 (PVI) is 0.466. (3) The value of correlation coefficient is low between the biomass and the vegetation indexes (RVI and NDVI) and the value of linear regression equation's R2 is 0.342 and 0.316, and the Logarithmic regression equation's R2 is 0.319 and 0.21, and the quadratic equation's R2 is 0.589 and 0.568, the value of correlation coefficient is high between the biomass and the vegetation indexes (PVI, SAVI and MSAVI), the value of linear regression equation is 0.626, 0.698 and 0.679, that of logarithmic regression equation is 0.592, 0.706 and 0.683 and that of the quadratic equation is 0.688, 0.711 and 0.683.

Ecosystem health assessment of the Jinghe River Watershed on the Huangtu Plateau.

An improved Costanza model was developed to assess the health of the Jinhe River Watershed ecosystem. The watershed is located at the center of the Huangtu Plateau in China and has suffered a severe disturbance in the last few decades. Three indicators including vigor, organization, and resilience were calculated respectively by merging ground-based observations with remotely sensed data on a watershed scale. Health indices of 12 topographic sub-watersheds were calculated using a modified Costanza formula. Health evaluated results indicated that sub-watersheds in the Huangtu mountain region were relatively healthy ecosystems with scores over 0.673. The sub-watersheds in the loess mountain and the loess gully regions, e.g., Jinghe, Heihe, and Honghe regions, scored moderately; their evaluated value ranged from 0.505 to 0.606. The two sub-watersheds in the loess gully region and all sub-watersheds in the loess hilly region scored the lowest, less than 0.50 and were considered unhealthy ecosystems. It can be argued that the loess hilly region and the loess gully regions should be in primary consideration for ecological protection and rehabilitation. This study provided a possible quantitative model for ecological planning and landscape management with respect to topographic conditions in this area.

[Empirical study on non-point sources pollution based on landscape pattern & ecological processes theory: a case of soil water loss on the Loess Plateau in China].

Non-point sources pollution is one of main pollution modes which pollutes the earth surface environment. Aimed at soil water loss (a typical non-point sources pollution problem) on the Losses Plateau in China, the paper applied a landscape patternevaluation method to twelve watersheds of Jinghe River Basin on the Loess Plateau by means of location-weighted landscape contrast index(LCI) and landscape slope index(LSI). The result showed that LSI of farm land, low density grass land, forest land and LCI responded significantly to soil erosion modulus and responded to depth of runoff, while the relationship between these landscape index and runoff variation index and erosion variation index were not statistically significant. This tell us LSI and LWLCI are good indicators of soil water loss and thus have big potential in non-point source pollution risk evaluation.

[Application of wavelet transform to monthly runoff time serial analysis in Zagunao watershed, the upper Minjiang river].

Runoff is an important component of regional water resources, and its dynamics is to some extent an indicator of water resources dynamics in a region. To know the runoff dynamics and water resources in a region is essential for the sustainable utilization and planning of water resources, and for the research on hydrological response of vegetation change at watershed scale. To disclose the water resources dynamics in Upper Minjiang River, one of the large reaches of Yangze River in southwest China, this paper analyzed the runoff dynamic features of Zagunao watershed, an important watershed in Minjiang River basin. Multi-Resolution Analysis (MRA) and periodicity analysis were conducted with Dmey wavelet function on the monthly runoff data from 1962 to 2002 observed by Zagunao hydrological station, which provided a data-based approximation on the evolution of monthly runoff in Zagunao watershed. It was found that the runoff dynamics in Zagunao watershed was relatively stable during 1962 - 1978, despite that this period was just in correspondence with the term of intensive deforestation activities in Upper Minjiang River basin. It was also clear that the runoff in Zagunao watershed was increased from 1986 to 1997, which was inconsistent with the commonly accepted viewpoint that the runoff decreased with increasing vegetation cover in forest watershed. However, the increasing trend from 1986 - 1997 was consistent with the research results in Yichang by Wang Wensheng and with the global warming at global and continent scale, which meant that global climate change plays a big role in runoff dynamics in Upper Minjiang River. Periodicity analysis showed that the rich-short water periodicity at the scale of 10 years (120 months) and 5 years (60 months) was 3 and 7 times, respectively, which could provide invaluable information for the eco-hydrological function research of forest landscape in Minjiang river basin and the sustainable utilization of water resources. The results from the case study highlighted that sometimes, it was very necessary to consider the role of global climatic warming in disclosing the hydrological response of land use/cover change at watershed scale, otherwise, it would be difficult to explain some hydrological phenomena.

[Relationships between soil and water loss and landscape pattern on Loess Plateau].

Based on the theories of detrended canonical correspondence analysis (DCCA), 12 soil and water loss indices ("species") and 7 landscape indices ("environmental factors") were adopted to quantitatively analyze the effects of landscape pattern on the soil and water loss in Jinghe River basin on Loess Plateau. The results showed that the first four DCCA axes were significantly correlated to agricultural land ratio, landscape diversity index, forest land ratio, and landscape contagion index. The characteristics of soil and water loss changed obviously with landscape pattern gradient, e. g., Sanshuihe watershed with a forest cover larger than 65% and low landscape diversity had a big and stable runoff, but small sediment and low river sand content. The runoff generation coefficient and runoff depth as well as the sediment transport and river sand content increased with the increasing agricultural land ratio and landscape diversity and the decreasing forest land ratio. As for Honghe watershed with a very low forest cover but high agricultural land ratio (53.41%), and with a relatively high landscape diversity and complex landscape pattern, it had the highest river sand content and the biggest sediment transport, and its monthly runoff and sediment transport varied markedly. The soil and water loss in the Upper-Huan, Down-Huan and Dongchuan watersheds with smaller forest and agricultural land ratio and relatively simple landscape pattern was smaller than that of the watersheds with dominated agricultural land.