Anthocyanins from Lycium ruthenicum Murr. Ameliorated d-Galactose-Induced Memory Impairment, Oxidative Stress, and Neuroinflammation in Adult Rats.

Lycium ruthenicum Murr. (LR) is a perennial shrub commonly used as a nutritional food and medicine. Herein, we identified 12 anthocyanins from LR, with petunidin derivatives constituting approximately 97% of the total anthocyanin content. Furthermore, the potential mechanism of anthocyanins exerting neuroprotective effects in d-galactose (d-gal)-treated rats was explored. Behavioral results showed that anthocyanins relieved d-gal-induced memory disorder. Additionally, anthocyanins reduced receptor for advanced glycation end products (RAGE) and suppressed oxidative stress caused by d-gal. Anthocyanins suppressed microgliosis and astrocytosis and reduced the overexpression of nuclear factor kappa B (NF-κB), interleukin-1-ß (IL-1ß), cyclooxygenase-2 (COX-2), and tumor necrosis factor-α (TNF-α). Moreover, anthocyanins lowered C-jun N-terminal kinase ( p-JNK), caspase-3 levels, and the B-cell lymphoma 2-associated X protein/B-cell lymphoma 2 (Bax/Bcl-2) ratio. Thus, anthocyanins from LR attenuated memory disfunction, neuroinflammation, and neurodegeneration caused by d-gal, possibly through the RAGE/NF-κB/JNK pathway, representing a promising, safe candidate for prevention and therapy of neurodegenerative diseases.

Interfacial assembly of mussel-inspired polydopamine@Ag core-shell nanoparticles as highly recyclable catalyst for nitroaromatic pesticides degradation.

With the abundant use of pesticides in agriculture and grassland farming, water contamination unavoidably occurs due to the leaching of pesticide residues into environment. Now, most of the research on degradation of nitroaromatic pesticides residues has focused on nano catalysis-based method, however, effective post-reaction separation and recycling of the tiny nanocatalysts is also a significant technological challenge to be addressed. Herein, we report a simple and versatile strategy for the construction of efficient and recyclable catalysts of polydopamine (PDA)@Ag/polystyrene (PS) films for degradation of pesticides based on multi-purpose PDA microspheres inspired by mussel adhesion. The PDA not only functioned as a scaffold, a reductant as well as a stabilizer for the formation and dispersions of Ag NPs in situ, but also served as an adhesive layer between the nanocatalysts and the substrate. The obtained PDA@Ag/PS films were evaluated for the first time against the catalytic degradation of pendimethalin. Most importantly, the recovery of catalysts can be easily realized by simply pulling out PS substrate from the reaction mixture and the catalytic activity of the nanofilms was found to be equally efficient for seven catalytic cycles. Considering their excellent catalytic activity and recyclability in the degradation of nitroaromatic pesticides, the PDA@Ag/PS films have great potential applications in the fields of environment protection, soil contamination remediation, and sewage treatment. Also, by virtue of the remarkable reducing and stabilizing ability and adhesive versatility of PDA, this approach can be extended to the deposition of various metals and semiconducting NPs, which can be stably anchored on a diverse range of solid substrates regardless of physiochemical and morphology.

Controllable conversion of Prussian blue@yeast bio-template into 3D cage-like magnetic Fe3O4@N-doped carbon absorbent and its cohesive regeneration by persulfate activation.

A multitude of heteroatom-doped carbon adsorbents have been explored to cope with ever-growing organic pollution. However, development of these advanced carbon materials with adequate activity and stability remains challenging. Herein, unique 3D cage-like magnetic N-doped Fe3O4@C adsorbents were rationally constructed by a one-step pyrolysis of Prussian blue@yeast (PB@yeast) bio-templates. By using yeast as an available biological support, the prepared Fe3O4@C hybrids were demonstrated to provide a sufficient number of Fe, N and C atoms for the novel cage-like microstructures, making them a new type of Fe, N co-doped carbon absorbents with a facile preparation procedure and remarkable adsorption behavior. Rhodamine B (RhB) removal indicated that the prepared N-doped Fe3O4@C adsorbents displayed high adsorption capabilities in a near-neutral solution, and Fe3O4@C (1 : 0.11) exhibited a maximum adsorption capability of 257.06 mg g-1. More importantly, spent N-doped Fe3O4@C absorbents, which could be recovered by magnetic separation and cohesive persulfate (PS) activated photo-Fenton regeneration, showed excellent adsorption reusability and high stability even after 5 cycles. Overall, this paper presents a simple method for fabrication of a 3D cage-like magnetic N-doped Fe3O4@C adsorbent, which provides a significant guidance for the study of Fe, N co-doped carbon adsorbents towards dye wastewater treatment.

Microbial synthesis of hollow porous Prussian blue@yeast microspheres and their synergistic enhancement of organic pollutant removal performance.

In this work, Prussian blue nanoparticles (PB NPs) were in situ grown on S. cerevisiae cells via one-step hydrothermal synthesis and the as-prepared Prussian blue@yeast (PB@yeast) hybrids exhibited synergistic adsorption and Fenton catalytic activities. FE-SEM, XRD and BET analysis of the prepared samples confirmed the successful formation of hollow porous structured PB@yeast microspheres, while FT-IR and XPS spectra indicated the fine structures were occupied by both functional adsorptive and catalytic sites. The experimental results of adsorption coupled Fenton reaction of PB@yeast hybrid microspheres revealed that the functional groups on the cell wall and the active iron sites in PB framework were fully utilized due to the triple synergistic effects of adsorption-Fenton catalysis-adsorption sites regeneration, thus endowing synergistically enhanced performance in removal of the selected cationic methylene blue (MB), anionic Methyl Orange (MO) and fluorescent brightener 71 (CXT) in aqueous solution. The high Fenton catalytic efficiency was related to the improvement of adsorption, in which the enrichment of contaminant molecules on the outer and inner surface of the hollow porous microspheres could lower mass transfer resistance and shorten charge transport pathways, thereby introducing more efficient Fenton catalytic activity than PB NPs.

A highly sensitive and selective method for determination of phenoxy carboxylic acids from environmental water samples by dispersive solid-phase extraction coupled with ultra high performance liquid chromatography-tandem mass spectrometry.

A rapid and efficient method for extraction of 12 phenoxy carboxylic acids (PCAs) in environmental water samples was established based on metal-organic framework MIL-101 assisted dispersive solid phase extraction (DSPE). 12 PCAs were labeled by d0-10-methyl-2-(piperazin-1-ylsulfonyl)anthracen-9(10H)-one(d0-MASPz) and d3-10-methyl-2-(piperazin-1-ylsulfonyl)anthracen-9(10H)-one(d3-MASPz), allowing each analyte to have an isotope internal standard. A stable isotope-coded strategy for the detection of 12 PCAs was developed under optimized extraction conditions and UHPLC-MS/MS conditions. All PCAs analytes were in good linearity in the concentration range of 5-1000 ng/L, and the calibration function was verified by the Mandel fitting test with a 95% confidence level. The LODs and LOQs were estimated by the IUPAC's recommendations. The LODs ranged from 0.18 to 0.88 ng/L, and LOQs ranged from 0.59 to 2.90 ng/L. The intra-day and inter-day precision in three spiked levels (5, 50 and 100 ng/L) were in the range of (1.40 ±â€¯0.14) %- (2.76 ±â€¯0.12) % and (2.60 ±â€¯0.26) %- (3.83 ±â€¯0.32) %, respectively. The developed method has been successfully applied to the determination of PCAs in environmental water samples with recoveries ranging from 95.3% to 105.5%. All of the precision and recovery analyses were done in triplicate.

Patulin removal from apple juice using a novel cysteine-functionalized metal-organic framework adsorbent.

Patulin (PAT) is one of the most common toxic contaminants of apple juice, which causes severe food safety issues throughout the apple industry. In order to remove PAT efficiently, a metal-organic framework-based adsorbent (UiO-66(NH2)@Au-Cys) was successfully synthesized and used for PAT removal from juice-pH simulation solution and real apple juice. Batch adsorption experiments were systematically performed to study the adsorption behavior for PAT. The results showed that adsorption process could be well described by the Pseudo-second order model and Freundlich isotherm model. The maximum adsorption capacity (4.38 µg/mg) was 10 times higher than the microbe-based biosorbents. Thermodynamic investigation demonstrated that adsorption process was spontaneous and endothermic. Furthermore, no marked cytotoxicity on NIH 3T3 cell lines was observed when the concentration of the adsorbent was lower than 10 µg/mL. Therefore, UiO-66(NH2)@Au-Cys is a potential adsorbent for PAT removal from apple juice with little quality changes.

Comparison of Phenols Content and Antioxidant Activity of Fruits from Different Maturity Stages of Ribes stenocarpum Maxim.

Differences in the content of nine phenols and the antioxidant capacity of Ribes stenocarpum Maxim (RSM) fruits at different stages of maturity were investigated, and the extraction process of polyphenols from RSM was also optimized using Box-Behnken design method. Results showed that the content of the nine phenols varied considerably at different ripening stages; catechin, chlorogenic acid, coumaric acid, and ferulic acid were abundant in immature fruits but decreased with fruit ripening, whereas the levels of rosemary acid and querctin acid were low in immature fruits and increased with time, reaching the highest value after the fruit was completely mature. The phenols extracted from RSM fruits possessed good antioxidant activities for effective and rapid scavenging of DPPH and ABTS free radicals, as well as intracellular ROS. Analysis of the phenols content at different maturity stages indicated that the unripe fruits had significantly higher polyphenols content than mature fruits. Consequently, unripe fruits possessed higher antioxidant activities. According to the overall results of the extraction process optimization, the selected optimal conditions for extracting polyphenols from RSM were as follows: extraction time, 95 min; solvent concentration, 60%; ratio of sample to solvent, 1:25.

Ultrasensitive colorimetric sensing strategy based on ascorbic acid triggered remarkable photoactive-nanoperoxidase for signal amplification and its application to α-glucosidase activity detection.

Recently, exploitation of nanozymes for signal amplification has aroused extensive research interesting in the fields of analytical chemistry and nanoscience. Herein, we introduced a new sensing strategy based on the ascorbic acid (AA) precisely regulated Ag3PO4 nanozyme for signal amplification. AA can reduce the partial Ag+ on the surface of Ag3PO4 nano-particles to form Ag° particles and result in the formation of Ag°/Ag3PO4 heterostructure. Due to surface plasmon resonance (SPR), the Ag° particles in heterostructure can act as the co-catalysts to enhance the electron-hole separation and the interfacial charge transfer, leading to a highly efficient oxidase mimicking activity for catalyzing the oxidation of the substrate of 3,3,5,5-tetramethylbenzidine (TMB) under visible light. Impressively, the activity enhancement of Ag3PO4 nano-peroxidase was linearly depended on the AA concentrations. Based on this feature, we employed it for multiple biological detections. For example, L-ascorbic acid-2-O-α-D-glucopyranosyl (AAG) was designed for α-glucosidase substrate, which can be hydrolyzed by α-glucosidase and lead to AA release. Further, the signal of α-glucosidase activity can be efficiently enlarged by Ag3PO4 nanozyme. Taking advantages of the powerful signal amplification by Ag3PO4 nanozymes, we developed the multiple sensing assays for monitoring AA in rat brain microdialysates, detection of α-glucosidase activity and its inhibitors (anti-diabetic drugs). The assay allowed the ultrasensitive colorimetric detection of α-glucosidase inhibitor with an ultra-low detection limit of 10 nM, and a naked-eye detection of the inhibitor concentration as low as 1 µM. The sensing strategy based on AA precisely regulated Ag3PO4 nano-peroxidase provides a promising candidate platform for highly stable and efficient cascaded signal amplification in biosensing field.

Rapid qualitative and quantitative analyses of eighteen phenolic compounds from Lycium ruthenicum Murray by UPLC-Q-Orbitrap MS and their antioxidant activity.

Lycium ruthenicum Murray (LR) is a functional food, and it has long been used in traditional folk medicine. However, detailed qualitative and quantitative analyses related to its phenolic compounds remains scarce. This work reports, for the first time, the establishment of a rapid method for simultaneous identification and quantification of 25 phenolic compounds by UPLC-quadrupole-Orbitrap mass spectrometry (UPLC-Q-Orbitrap MS). This method was validated by LODs, LOQs, precision, repeatability, stability, mean recovery, recovery range and RSD. The confirmed method was applied to the analysis of phenolic compounds in LR. Finally, 18 phenolic compounds in LR were qualitatively and quantitatively analyzed. Among them, 11 constituents were detected for the first time, which included two flavonoids (catechin and naringenin) and seven phenolic acids (gallic acid, vanillic acid, 2,4-dihydroxybenzoic acid, veratronic acid, benzoic acid, ellagic acid and salicylic acid). Moreover, Phloretin and protocatechuate, belonging to the dihydrochalcone flavonoid and protocatechuic acid respectively, were also identified and quantified. The total phenolics content (20.17 ±â€¯2.82 mg/g) and the total anthocyanin content (147.43 ±â€¯1.81 mg/g) were determined. In addition, the antioxidant activities of the LR extract were evaluated through 2,2-azinobis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radical scavenging activity, ferric reducing antioxidant power (FRAP) and total antioxidant activity (T-AOC) assays.

Biomass reinforced graphene oxide solid/liquid phase membrane extraction for the measurement of Pb(II) in food samples.

Here, a solid/liquid phase extraction mode was used for the preconcentration of Pb(II) in water and food samples through a biomass reinforced graphene oxide (BGO) membrane. Taking advantage of the two modes, the synergistic effect of BGO membrane (solid extraction) and organic solvent (liquid extraction) enhances the extraction efficiency toward target ion. In detail, the effect of composition parameters in BGO membrane and experiment conditions such as pH, eluent types, elution time and sample volume were optimized, as well as shows no obvious interference toward different competing ions. Under the optimal experiment conditions, the limit of detection, precision as RSD% of this method were found to be 0.84 µg L-1 and 4.65%, respectively. Moreover, the large enrichment factor of BGO membrane demonstrates the applicability of the use of large sample volumes. Furthermore, the method was successfully verified by analyzing spiked Pb(II) in water and food samples.

Polyphenol Stilbenes from Fenugreek (Trigonella foenum-graecum L.) Seeds Improve Insulin Sensitivity and Mitochondrial Function in 3T3-L1 Adipocytes.

Fenugreek (Trigonella foenum-graecum L.) is a well-known annual plant that is widely distributed worldwide and has possessed obvious hypoglycemic and hypercholesterolemia characteristics. In our previous study, three polyphenol stilbenes were separated from fenugreek seeds. Here, we investigated the effect of polyphenol stilbenes on adipogenesis and insulin resistance in 3T3-L1 adipocytes. Oil Red O staining and triglyceride assays showed that polyphenol stilbenes differently reduced lipid accumulation by suppressing the expression of adipocyte-specific proteins. In addition, polyphenol stilbenes improved the uptake of 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG) by promoting the phosphorylation of protein kinase B (AKT) and AMP-activated protein kinase (AMPK). In present studies, it was found that polyphenol stilbenes had the ability to scavenge reactive oxygen species (ROS). Results from adenosine triphosphate (ATP) production and mitochondrial membrane potentials suggested that mitochondria play a critical role in insulin resistance and related signaling activation, such as AKT and AMPK. Rhaponticin, one of the stilbenes from fenugreek, had the strongest activity among the three compounds in vitro. Future studies will focus on mitochondrial biogenesis and function.

[Preparation of Prussian Blue@Yeast Catalyst and Its Heterogeneous Fenton Performance].

A novel heterogeneous Fenton catalyst, Prussian blue@yeast (PB@yeast), was prepared via facile self-assembly synthesis. The as-synthesized composite was characterized by field emission scanning electron microscopy (FE-SEM), energy-dispersive spectroscopy (EDS), powder X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR). All of the results indicated that PB nanoparticles were uniformly dispersed on the surface of yeasts with stable core-shell morphology. Degradation of the model anionic fluorescent whitening agent CXT indicated that the PB@yeast catalyst presented a synergistic effect of adsorption and heterogeneous Fenton performance. Owing to the high adsorption capacity of yeast, the CXT molecules were easy to move to the active site of the catalyst, promoting the electron transfer between Fe(Ⅲ) and Fe(Ⅱ) and then enhancing the catalytic activity of the Fenton reaction effectively. Furthermore, the yeast support could improve the dispersity and stability of PB nanoparticles, which maintained excellent catalytic activity and stability after being recycled four times.

Synthesis of Porous CoFe2O4 and Its Application as a Peroxidase Mimetic for Colorimetric Detection of H2O2 and Organic Pollutant Degradation.

Porous CoFe2O4 was prepared via a simple and controllable method to develop a low-cost, high-efficiency, and good-stability nanozyme. The morphology and microstructure of the obtained CoFe2O4 was investigated by X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), transmission electron microscopy (TEM), high-resolution TEM (HRTEM), specific surface area and pore analysis, and Raman spectroscopy. The results show that the annealing temperature has an important effect on the crystallinity, grain size, and specific surface area of CoFe2O4. CoFe2O4 obtained at 300 °C (CF300) exhibits the largest surface area (up to 204.1 m² g−1) and the smallest grain size. The peroxidase-like activity of CoFe2O4 was further verified based on the oxidation of peroxidase substrate 3,3’,5,5’-tetramethylbenzidine (TMB) in the presence of H2O2. The best peroxidase-like activity for CF300 should be ascribed to its largest surface area and smallest grain size. On this basis, an effective method of colorimetric detection H2O2 was established. In addition, the porous CoFe2O4 was also used for the catalytic oxidation of methylene blue (MB), indicating potential applications in pollutant removal and water treatment.

A novel and sensitive method for determining vitamin B3 and B7 by pre-column derivatization and high-performance liquid chromatography method with fluorescence detection.

A new labeling reagent for vitamin analysis, 2-amino-10-ethyl acridine ketone (AEAO), has been synthesized and successfully applied to the analysis of vitamin B3 and vitamin B7 in different tea samples. The reaction of AEAO with vitamins could proceed easily and quickly in the presence of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC) as condensing reagent within 45 min. The derivatives exhibited excellent fluorescence property with excitation and emission wavelengths of 290 nm and 430 nm, respectively. Response surface methodology (RSM) was applied to the optimization of pre-column derivatization. Solid phase extraction with HLB cartridges was used for the extraction and purification of water-soluble vitamins in tea samples. The LODs for vitamin B3 and vitamin B7 were 2.56 and 2.22 ng mL-1, respectively. The proposed method was successfully applied to the analysis of vitamin B3 and vitamin B7 in different tea samples. The study provided a highly sensitive method for accurate analysis of trace vitamins from natural products.

Conductive Leaflike Cobalt Metal-Organic Framework Nanoarray on Carbon Cloth as a Flexible and Versatile Anode toward Both Electrocatalytic Glucose and Water Oxidation.

Transition metal-organic frameworks (MOFs), on account of their unique inherent properties of large pore volume, high specific surface area, tunable pores, and good catalytic activity, have been highly regarded as superior catalysts recently for water electrolysis, supercapacitors, batteries, sensors, and so on. Herein, we report on a cobalt MOF phase with 3D well-aligned nanosheets array architecture on carbon cloth (Co-MOF NS/CC), fabricated by a facile ambient liquid-phase deposition, could serve as a self-standing Janus catalytic electrode toward both glucose and water oxidation. It shows good glucose-sensing performance with low determination limit and large detection range. Also, it exhibits high water-oxidation efficiency with low overpotential and good durability. This work demonstrates the potential of utilizing transition-metal based well-aligned MOF nanoarrays for electrocatalytic oxidation.

Molecular Sex Identification in Dioecious Hippophae rhamnoides L. via RAPD and SCAR Markers.

The dioecious property of the sea buckthorn (Hippophae rhamnoides L.) prevents sex recognition via traditional observation at the juvenile stage, thus impeding breeding and economic cropping; A random amplified polymorphic DNA (RAPD) and a sequence characterized amplified region (SCAR) markers were used to identify the sexes. A total of 45 random decamer primers were used to screen genomic DNA pools of staminate and pistillate genotypes for genetic polymorphisms. One female sex-linked marker was identified. D15 (5'-CATCCGTGCT-3') amplified a particular band of 885 bp, which showed polymorphism among staminate and pistillate genotype plants. The SCAR marker Hrcx-15 was obtained by sequencing the fragment. The alleles of 140 pistillate genotypes were examined but not of the 140 staminate genotypes discerned via taxonomy. Staminate and pistillate genotypes of sea buckthorn plants can be distinguished, using Hrcx-15 as a genetic marker for sex identification and for expediting cultivation for commercial applications.

An improved clenbuterol detection by immunochromatographic assay with bacteria@Au composite as signal amplifier.

Immunochromatographic assays (ICAs) are most frequently used for on-site rapid screening of clenbuterol. To improve sensitivity, a novel probe with bacteria as signal carriers was developed. Bacteria can load a great deal of gold nanoparticles (AuNPs) on their surface, meaning much fewer antibodies are needed to produce clearly visible results, although low concentrations of antibody could also trigger fierce competition between free analyte and the immobilized antigen. Thus, a limited number of antibodies was key to significantly improved sensitivity. Analytical conditions, including bacterial species, coupling method, and concentration, were optimized. The visual detection limit (VDL) for clenbuterol was 0.1 ng/mL, a 20-fold improvement in sensitivity compared with traditional strips. This work has opened up a new route for signal amplification and improved performance of ICAs. Furthermore, inactivated bacteria could also be environment-friendly and robust signal carriers for other biosensors.

Interfacial growth of nitrogen-doped carbon with multi-functional groups on the MoS2 skeleton for efficient Pb(II) removal.

Lead(II) is a highly toxic upon accumulation in the human body and a thorough cleanup of this pollutant in water resources has become a rising worldwide concern. In this study, multi-functional nitrogen-doped carbon-MoS2 (NC-MoS2) nanohybrid composite was successfully synthesized by a step-by-step approach and applied as an effective heavy metal ions adsorbent. Scanning electron microscopy and energy dispersive X-ray spectrometer analysis presents multilayer irregular nanosheets and reveals the uniform distribution of C, N and O elements in the fabricated nanomaterials, respectively. Powder X-ray diffraction, Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy confirm the functionalization of oxygen-containing functional groups arising from the nitrogen-doped carbon. Particularly, the NC-MoS2 possesses an excellent adsorption capacity (439.09mgg-1) for Pb(II), which attributes to the integrated physicochemical adsorption resulting from the oxygen-containing functional groups on the surface of nanohybrid composites. The as-prepared NC-MoS2 exhibits good chemical stability and can be reused 7 times with a slight fading over 80% during cycling, which is essential for its practical applications. All of these demonstrate that NC-MoS2 can be an efficient material for selectively removal of Pb(II), which suggest its great potential in the utilization of environmental cleanup.

Triterpenic Acids from Potentilla parvifolia and Their Protective Effects against Okadaic Acid Induced Neurotoxicity in Differentiated SH-SY5Y Cells.

Six triterpenic acids were separated and purified from the ethyl acetate extractive fraction of ethanol extracts of Potentilla parvifolia FISCH. using a variety of chromatographic methods. The neuroprotective effects of these triterpenoids were investigated in the present study, in which the okadaic acid induced neurotoxicity in human neuroblastoma SH-SY5Y cells were used as an Alzheimer's disease cell model in vitro. The cell model was established with all trans-retinoic acid (5 µmol/L, 4 d) and okadaic acid (40 nmol/L, 6 h) treatments to induce tau phosphorylation and synaptic atrophy. Subsequently, the neuroprotective effects of these triterpenic acids were evaluated in vitro by this cell model. Results from the Western blot and morphology analysis suggested that compounds 3-6 had the better neuroprotective effects. Furthermore, we tested the level of mitochondrial reactive oxygen species and mitochondrial membrane potential of these compounds in SH-SY5Y cells by flow cytometry technology to investigate the potential neuroprotective mechanism of these compounds. All of the results indicated that maybe the mechanism of compounds 5 and 6 is to protect the cell from mitochondrial oxidative stress injuries.

Natural Sugar: A Green Assistance To Efficiently Exfoliate Inorganic Layered Nanomaterials.

We have demonstrated that natural sugars can efficiently exfoliate inorganic layered nanomaterials with direct stirring. The representative transition-metal dichalcogenides (MoS2 and WS2), transition-metal oxide (MoO3), and graphene were explored, and the formation of ultrathin nanosheets was verified. Glucose and MoS2 selected each other as the perfect partner with superior exfoliation and excellent properties. The obtained inorganic layered nanosheets possess favorable stability and dispersity, which renders it suitable for direct homogeneous liquid applications, such as catalytic activities and sensors. With a high-throughput and green process, the sugar-assisted method may offer new ideas for inorganic layered nanomaterials synthesis and applications in a more ecofriendly way.